The cytoplasmic droplet may be indicative of sperm motility and normal spermiogenesis

Asian Journal of Andrology

Volumn 15, Issue 6, 2013, Pages 799-805

  Xu, Hui ^a   Yuan, Shui Qiao ^a,b   Zheng, Zhi Hong ^a   Yan, Wei ^a,b  

^a CHINA MEDICAL UNIVERSITY   (China)

^b UNIVERSITY OF NEVADA SCHOOL OF MEDICINE   (United States)

Author keywords

cytoplasmic droplets; epididymis; fertility; mouse; semen analysis; sperm motility; spermiogenesis

Indexed keywords

ARTICLE; CELL ORGANELLE; CELL STRUCTURE; CONTROLLED STUDY; CYTOPLASM; EPIDIDYMIS; HAPLORHINI; MACACA FASCICULARIS; MALE; MOUSE; NONHUMAN; SPERMATID; SPERMATOGENESIS; SPERMATOZOON MATURATION; SPERMATOZOON MOTILITY; SPERMATOZOON TAIL;

ANIMALS; CYTOPLASM; MALE; MICE; MICE, INBRED STRAINS; SPERM MOTILITY; SPERMATOGENESIS;

EID: 84887249573     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1038/aja.2013.69     Document Type: Article

References (33)

1. Hermo L, Pelletier RM, Cyr DG, Smith CE. Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: background to spermatogenesis, spermatogonia, and spermatocytes. Microsc Res Tech 2010; 73: 241-78.
2. Dott HM, Dingle JT. Distribution of lysosomal enzymes in the spermatozoa and cytoplasmic droplets of bull and ram. Exp Cell Res 1968; 52: 523-40.
3. Bavdek S, Glover TD. Alkaline phosphatase in the cytoplasmic droplet of rabbit spermatozoa. J Reprod Fertil 1970; 22: 371-3.
4. Garbers DL, Wakabayashi T, Reed PW. Enzyme profile of the cytoplasmic droplet from bovine epididymal spermatozoa. Biol Reprod 1970; 3: 327-37.
5. Moniem KA, Glover TD. Alkaline phosphatase in the cytoplasmic droplet of mammalian spermatozoa. J Reprod Fertil 1972; 29: 65-9.
6. Roberts ML, Scouten WH, Nyquist SE. Isolation and characterization of the cytoplasmic droplet in the rat. Biol Reprod 1976; 14: 421-4.
7. Retzius G. Die Spermien der Huftiere. Biol Untersuch NFH 1909; 14: 163-78.
8. Burgos MH, Fawcett DW. Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica).J Biophys Biochem Cytol 1955; 1: 287-300.
9. Oko R, Hermo L, Chan PT, Fazel A, Bergeron JJ. The cytoplasmic droplet of rat epididymal spermatozoa contains saccular elements with Golgi characteristics. J Cell Biol 1993; 123: 809-21. (Pubitemid 23333571)
10. Chen Q, Peng H, Lei L, Zhang Y, Kuang H et al. Aquaporin3 is a sperm water channel essential for postcopulatory sperm osmoadaptation and migration. Cell Res 2011; 21: 922-33.
11. Cooper TG. The epididymis, cytoplasmic droplets and male fertility. Asian J Androl 2011; 13: 130-8.
12. Fetic S, Yeung CH, Sonntag B, Nieschlag E, Cooper TG. Relationship of cytoplasmic droplets to motility, migration in mucus, and volume regulation of human spermatozoa. J Androl 2006; 27: 294-301.
13. Yeung CH, Callies C, Rojek A, Nielsen S, Cooper TG. Aquaporin isoforms involved in physiological volume regulation of murine spermatozoa. Biol Reprod 2009; 80: 350-7.
14. Cooper TG. Cytoplasmic droplets: the good, the bad or just confusing? Hum Reprod 2005; 20: 9-11.
15. Cooper TG, Yeung CH. Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet. Microsc Res Tech 2003; 61: 28-38. (Pubitemid 36457544)
16. Cooper TG, Yeung CH, Fetic S, Sobhani A, Nieschlag E. Cytoplasmic droplets are normal structures of human sperm but are not well preserved by routine procedures for assessing sperm morphology. Hum Reprod 2004; 19: 2283-8. (Pubitemid 39406689)
17. Hermo L, Pelletier RM, Cyr DG, Smith CE. Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane. Microsc Res Tech 2010; 73: 320-63.
18. Zini A, Defreitas G, Freeman M, Hechter S, Jarvi K. Varicocele is associated with abnormal retention of cytoplasmic droplets by human spermatozoa. Fertil Steril 2000; 74: 461-4.
19. Turner RM. Moving to the beat: a review of mammalian sperm motility regulation. Reprod Fertil Dev 2006; 18: 25-38. (Pubitemid 41831073)
20. Ola B, Afnan M, Papaioannou S, Sharif K, Bjorndahl L et al. Accuracy of sperm-cervical mucus penetration tests in evaluating sperm motility in semen: a systematic quantitative review. Hum Reprod 2003; 18: 1037-46. (Pubitemid 36592047)
21. Zheng H, Stratton CJ, Morozumi K, Jin J, Yanagimachi R et al. Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation, and male infertility. Proc Natl Acad Sci USA 2007; 104: 6852-7. (Pubitemid 47175590)
22. Jin J, Jin N, Zheng H, Ro S, Tafolla D et al. Catsper3 and Catsper4 are essential for sperm hyperactivated motility and male fertility in the mouse. Biol Reprod 2007; 77: 37-44. (Pubitemid 46986394)
23. Yan W. Male infertility caused by spermiogenic defects: lessons from gene knockouts. Mol Cell Endocrinol 2009; 306: 24-32.
24. Hermo L, Dworkin J, Oko R. Role of epithelial clear cells of the rat epididymis in the disposal of the contents of cytoplasmic droplets detached from spermatozoa. Am J Anatomy 1988; 183: 107-24.
25. Shirley CR, Hayashi S, Mounsey S, Yanagimachi R, Meistrich ML. Abnormalities and reduced reproductive potential of sperm from Tnp1-and Tnp2-null double mutant mice. Biol Reprod 2004; 71: 1220-9. (Pubitemid 39249807)
26. Zhao M, Shirley CR, Mounsey S, Meistrich ML. Nucleoprotein transitions during spermiogenesis in mice with transition nuclear protein Tnp1 and Tnp2 mutations. Biol Reprod 2004; 71: 1016-25. (Pubitemid 39108666)
27. Harrison RA, White IG. Glycolytic enzymes in the spermatozoa and cytoplasmic droplets of bull, boar and ram, and their leakage afte shock. J Reprod Fertil 1972; 30: 105-15.
28. Rengan AK, Agarwal A, van der Linde M, du Plessis SS. An investigation of excess residual cytoplasm in human spermatozoa and its distinction from the cytoplasmic droplet. Reprod Biol Endocrinol 2012; 10: 92.
29. Bao J, Wu Q, Song R, Jie Z, Zheng H et al. RANBP17 is localized to the XY body of spermatocytes and interacts with SPEM1 on the manchette of elongating spermatids. Mol Cell Endocrinol 2011; 333: 134-42.
30. Bao J, Zhang J, Zheng H, Xu C, Yan W. UBQLN1 interacts with SPEM1 and participates in spermiogenesis. Mol Cell Endocrinol 2010; 327: 89-97.
31. Iguchi N, Tanaka H, Yamada S, Nishimura H, Nishimune Y. Control of mouse hils1 gene expression during spermatogenesis: identification of regulatory element by transgenic mouse. Biol Reprod 2004; 70: 1239-45. (Pubitemid 38509668)
32. Yan W, Ma L, Burns KH, Matzuk MM. HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis. Proc Natl Acad Sci USA 2003; 100: 10546-51. (Pubitemid 37071915)
33. Jedrzejczak P, Kempisty B, Bryja A, Mostowska M, Depa-Martynow M et al. Quantitative assessment of transition proteins 1, 2 spermatid-specific linker histone H1-like protein transcripts in spermatozoa from normozoospermic and asthenozoospermic men. Arch Androl 2007; 53: 199-205. (Pubitemid 47402849)