A novel and robust recombinant Pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a Thermomyces lanuginosus lipase: Preparation, characterization and application in biodiesel production

Bioresource Technology

Volumn 151, Issue , 2014, Pages 43-48

  Yan, Jinyong ^a   Zheng, Xianliang ^a   Li, Shengying ^a  

^a QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

Author keywords

Biodiesel; Lipase; Pichia pastoris; Waste cooking oils; Whole cell catalyst

Indexed keywords

CATALYST ACTIVITY; CELLS; COST EFFECTIVENESS; CYTOLOGY; LIPASES; OILS AND FATS; REUSABILITY; YEAST;

BIODIESEL PRODUCTION; CATALYTIC PERFORMANCE; PICHIA PASTORIS; SHORT CHAIN ALCOHOLS; THERMOMYCES LANUGINOSUS LIPASE; WASTE COOKING OIL; WHOLE CELL BIOCATALYSTS; WHOLE-CELL CATALYSTS;

BIODIESEL;

BIODIESEL; FATTY ACID ESTER; METHANOL; OIL; TRIACYLGLYCEROL LIPASE;

BIOFUEL; CATALYST; CELL ORGANELLE; ENZYME ACTIVITY; GENE EXPRESSION; METHANOL; OIL PRODUCTION; RECOMBINATION; YEAST;

ALCOHOL TOLERANCE; ARTICLE; BIOCATALYST; BIOTRANSFORMATION; CATALYSIS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ENZYME ACTIVITY; FUNGAL STRAIN; MICROENVIRONMENT; PICHIA PASTORIS; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN EXPRESSION; REACTION TIME; RECYCLING; THERMOMYCES LANUGINOSUS; THERMOSTABILITY; WASTE; YEAST;

PICHIA PASTORIS; THERMOMYCES LANUGINOSUS;

BIODIESEL; BIOMASS SUPPORT PARTICLES; BSPS; DCW; DRY CELL WEIGHT; FAEES; FAMES; FATTY ACID ETHYL ESTERS; FATTY ACID METHYL ESTERS; FFAS; FREE FATTY ACIDS; LIPASE; PICHIA PASTORIS; WASTE COOKING OILS; WCCS; WHOLE CELL CATALYST; WHOLE CELL CATALYSTS;

ALCOHOLS; ASCOMYCOTA; BIOCATALYSIS; BIOFUELS; BIOTECHNOLOGY; BIOTRANSFORMATION; COOKING; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HYDROLYSIS; LIPASE; PICHIA; RECOMBINATION, GENETIC; RECYCLING; TEMPERATURE; TIME FACTORS; WASTE PRODUCTS;

EID: 84887178078     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2013.10.037     Document Type: Article

References (26)

1. Adachi D., Koh F.H., Hama S., Ogino C., Kondo A. A robust whole-cell biocatalyst that introduces a thermo- and solvent-tolerant lipase into Aspergillus oryzae cells: characterization and application to enzymatic biodiesel production. Enzyme Microb. Technol. 2013, 52:331-335.
2. Adachi D., Hama S., Nakashima K., Bogaki T., Ogino C., Kondo A. Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B. Bioresour. Technol. 2013, 135:410-416.
3. Ban K., Hama S., Nishizuka K., Kaieda M., Matsumoto T., Kondo A., Noda H., Fukuda H. Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production. J. Mol. Catal. B Enzym. 2002, 17:157-165.
4. Barbosa O., Torres R., Ortiz C., Berenguer-Murcia A., Rodrigues R.C., Fernandez-Lafuente R. Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties. Biomacromolecules 2013, 14:2433-2462.
5. Fernandez-Lafuente R. Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst. J. Mol. Catal. B Enzym. 2010, 62:197-212.
6. Fukuda H., Hama S., Tamalampudi S., Noda H. Whole-cell biocatalysts for biodiesel fuel production. Trends Biotechnol. 2008, 26:668-673.
7. Gao B., Su E.Z., Lin J.P., Jiang Z.B., Ma Y.S., Wei D.Z. Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production. J. Biotechnol. 2009, 139:169-175.
8. He Q., Xu Y., Teng Y., Wang D. Biodiesel production catalyzed by whole-cell lipase from Rhizopus chinensis. Chin. J. Catal. 2008, 29:41-46.se.
9. Huang D.F., Han S.Y., Han Z.l., Lin Y. Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system. Biochem. Eng. J. 2012, 63:10-14.
10. Kondo A., Liu Y., Furuta M., Fujita Y., Matsumoto T., Fukuda H. Preparation of high activity whole cell biocatalyst by permeabilization of recombinant flocculent yeast with alcohol. Enzyme Microb. Technol. 2000, 27:806-811.
11. Koda R., Numata T., Hama S., Tamalampudi S., Nakashima K., Tanaka T., Ogino C., Fukuda H., Kondo A. Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts. J. Mol. Catal. B Enzyme 2010, 66:101-104.
12. Li W., Du W., Liu D.H. Rhizopus oryzae IFO 4697 whole cell catalyzed methanolysis of crude and acidified rapeseed oils for biodiesel production in tert-butanol system. Process Biochem. 2007, 42:1481-1485.
13. Li A.T., Ngo T.P.N., Yan J.Y., Tian K.Y., Li Z. Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresour. Technol. 2012, 114:725-729.
14. Lam M.K., Lee K.T., Mohamed A.R. Homogeneous, heterogeneous and enzymatic catalysis for transesterification of high free fatty acid oil (waste cooking oil) to biodiesel: a review. Biotechnol. Adv. 2010, 28:500-518.
15. Matsumoto T., Fukuda H., Ueda M., Tanaka A., Kondo A. Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain. Appl. Environ. Microbiol. 2002, 68:4517-4522.
16. Matsumoto T., Takahashi S., Kaieda M., Ueda M., Tanaka A., Fukuda H., Kondo A. Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production. Appl. Microbiol. Biotechnol. 2001, 57:515-520.
17. Martín E.H., Otero C. Different enzyme requirements for the synthesis of biodiesel: Novozym 435 and Lipozyme TLIM. Bioresour. Technol. 2008, 99:277-286.
18. Patrick S.M., Fazenda M.L., McNeil B., Harvey L.M. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22:249-270.
19. Rodrigues R.C., Ortiz C., Berenguer-Murcia A., Torres R., Fernández-Lafuente R. Modifying enzyme activity and selectivity by immobilization. Chem. Soc. Rev. 2013, 42:6290-6307.
20. Takaya T., Koda R., Adachi D., Nakashima K., Wada J., Bogaki T., Ogino C., Kondo A. Highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in Aspergillus oryzae. Appl. Microbiol. Biotechnol. 2011, 90:1171-1177.
21. Tan T.W., Lu J.K., Nie K.L., Deng L., Wang F. Biodiesel production with immobilized lipase: a review. Biotechnol. Adv. 2010, 28:628-634.
22. Verma M.L., Barrow C.J., Puri M. Nanobiotechnology as a novel paradigm for enzyme immobilisation and stabilisation with potential applications in biodiesel production. Appl. Microbiol. Biotechnol. 2013, 97:23-39.
23. Xiao M., Qi C., Obbard J.P. Biodiesel production using Aspergillus niger as a whole cell biocatalyst in a packed-bed reactor. GCB Bioenergy 2011, 3:293-298.
24. Yan J.Y., Yang J.K., Xu L., Yan Y.J. Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05. J. Mol. Catal. B Enzym. 2007, 49:28-35.
25. Yan J.Y., Yan Y.J., Liu S.X., Hu J., Wang G.L. Preparation of cross-linked lipase-coated micro-crystals for biodiesel production from waste cooking oil. Bioresour. Technol. 2011, 102:4755-4758.
26. Yan J.Y., Li A.T., Xu Y., Ngo T.P.N., Phua S.C., Li Z. Efficient production of biodiesel from waste grease: one-pot esterification and transesterification with tandem lipases. Bioresour. Technol. 2012, 2012(123):332-337.