LysoTracker is a marker of differentiated alveolar type II cells

Respiratory Research

Volumn 14, Issue 1, 2013, Pages

  Van der Velden, Joanne L ^a   Bertoncello, Ivan ^a   McQualter, Jonathan L ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Alveolar type II cells; Cell culture; Differentiation; Flow Cytometry; Lung; LysoTracker

Indexed keywords

CD31 ANTIGEN; CD45 ANTIGEN; EPITHELIAL CELL ADHESION MOLECULE; FLUORESCENT DYE; LYSOTRACKER GREEN DND 26; SURFACTANT PROTEIN C; UNCLASSIFIED DRUG; AMINE; RED DND 99; RED DND-99;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; C57BL 6 MOUSE; CELL ACTIVITY; CELL DIFFERENTIATION; CELL FUNCTION; CELL ISOLATION; CELL POPULATION; CELL SELECTION; CELL SORTER; CELL STRUCTURE; CELL SUSPENSION; CELL VIABILITY; COLONY FORMING UNIT; COMPARATIVE STUDY; EPITHELIUM CELL; FEMALE; FLOW CYTOMETRY; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LAMELLAR BODY; LUNG ALVEOLUS CELL TYPE 2; MOUSE; NONHUMAN; OUTCOME ASSESSMENT; PROTEIN EXPRESSION; SECRETORY CELL; STEM CELL; ANIMAL; ANIMAL MODEL; C57BL MOUSE; CELL CULTURE; CELL SURVIVAL; CYTOLOGY; EVALUATION STUDY; LUNG; LUNG ALVEOLUS; METHODOLOGY;

AMINES; ANIMALS; CELL DIFFERENTIATION; CELL SURVIVAL; CELLS, CULTURED; FEMALE; FLOW CYTOMETRY; FLUORESCENT DYES; LUNG; MICE; MICE, INBRED C57BL; MODELS, ANIMAL; PULMONARY ALVEOLI;

EID: 84887152600     PISSN: 14659921     EISSN: 1465993X     Source Type: Journal    
DOI: 10.1186/1465-9921-14-123     Document Type: Article

References (19)

1. Adamson IY, Bowden DH. Derivation of type 1 epithelium from type 2 cells in the developing rat lung. Lab Invest 1975, 32:736-745.
2. Evans MJ, Cabral LJ, Stephens RJ, Freeman G. Transformation of alveolar type 2 cells to type 1 cells following exposure to NO2. Exp Mol Pathol 1975, 22:142-150. 10.1016/0014-4800(75)90059-3, 163758.
3. Dobbs LG, Gonzalez R, Williams MC. An improved method for isolating type II cells in high yield and purity. Am Rev Respir Dis 1986, 134:141-145.
4. Fujino N, Kubo H, Ota C, Suzuki T, Suzuki S, Yamada M, Takahashi T, He M, Suzuki T, Kondo T, Yamaya M. A novel method for isolating individual cellular components from the adult human distal lung. Am J Respir Cell Mol Biol 2012, 46:422-430. 10.1165/rcmb.2011-0172OC, 22033268.
5. Gereke M, Autengruber A, Grobe L, Jeron A, Bruder D, Stegemann-Koniszewski S. Flow cytometric isolation of primary murine type II alveolar epithelial cells for functional and molecular studies. J Vis Exp 2012, 70:e4322.
6. Abraham V, Chou ML, DeBolt KM, Koval M. Phenotypic control of gap junctional communication by cultured alveolar epithelial cells. Am J Physiol 1999, 276:L825-L834.
7. Chen J, Chen Z, Narasaraju T, Jin N, Liu L. Isolation of highly pure alveolar epithelial type I and type II cells from rat lungs. Lab Invest 2004, 84:727-735. 10.1038/labinvest.3700095, 15077123.
8. Lee JH, Kim J, Gludish D, Roach RR, Saunders AH, Barrios J, Woo AJ, Chen H, Conner DA, Fujiwara Y, et al. SPC H2B-GFP mice reveal heterogeneity of surfactant protein C-expressing lung cells. Am J Respir Cell Mol Biol 2012, 48(3):288-298.
9. Haller T, Ortmayr J, Friedrich F, Volkl H, Dietl P. Dynamics of surfactant release in alveolar type II cells. Proc Natl Acad Sci U S A 1998, 95:1579-1584. 10.1073/pnas.95.4.1579, 19102, 9465058.
10. Jennings P, Bertocchi C, Frick M, Haller T, Pfaller W, Dietl P. Ca2+ induced surfactant secretion in alveolar type II cultures isolated from the H-2Kb-tsA58 transgenic mouse. Cell Physiol Biochem 2005, 15:159-166. 10.1159/000083648, 15665525.
11. McQualter JL, Yuen K, Williams B, Bertoncello I. Evidence of an epithelial stem/progenitor cell hierarchy in the adult mouse lung. Proc Natl Acad Sci U S A 2010, 107:1414-1419. 10.1073/pnas.0909207107, 2824384, 20080639.
12. Bertoncello I, McQualter J. Isolation and clonal assay of adult lung epithelial stem/progenitor cells. Curr Protoc Stem Cell Biol 2011, Chapter 2:Unit 2G 1.
13. Dobbs LG. Isolation and culture of alveolar type II cells. Am J Physiol 1990, 258:L134-147.
14. Barkauskas CE, Cronce MJ, Rackley CR, Bowie EJ, Keene DR, Stripp BR, Randell SH, Noble PW, Hogan BL. Type 2 alveolar cells are stem cells in adult lung. J Clin Invest 2013, 123:3025-3036. 10.1172/JCI68782, 3696553, 23921127.
15. Chapman HA, Li X, Alexander JP, Brumwell A, Lorizio W, Tan K, Sonnenberg A, Wei Y, Vu TH. Integrin alpha6beta4 identifies an adult distal lung epithelial population with regenerative potential in mice. J Clin Invest 2011, 121:2855-2862. 10.1172/JCI57673, 3223845, 21701069.
16. Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T. Identification of bronchioalveolar stem cells in normal lung and lung cancer. Cell 2005, 121:823-835. 10.1016/j.cell.2005.03.032, 15960971.
17. Roper JM, Staversky RJ, Finkelstein JN, Keng PC, O'Reilly MA. Identification and isolation of mouse type II cells on the basis of intrinsic expression of enhanced green fluorescent protein. Am J Physiol Lung Cell Mol Physiol 2003, 285:L691-700.
18. Teisanu RM, Chen H, Matsumoto K, McQualter JL, Potts E, Foster WM, Bertoncello I, Stripp BR. Functional analysis of two distinct bronchiolar progenitors during lung injury and repair. Am J Respir Cell Mol Biol 2011, 44:794-803. 10.1165/rcmb.2010-0098OC, 3135841, 20656948.
19. Ichimura H, Parthasarathi K, Lindert J, Bhattacharya J. Lung surfactant secretion by interalveolar Ca2+ signaling. Am J Physiol Lung Cell Mol Physiol 2006, 291:L596-601. 10.1152/ajplung.00036.2006, 16698857.