Crystal structure of a glucose/H+ symporter and its mechanism of action

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 44, 2013, Pages 17862-17867

  Iancu, Cristina V ^a   Zamoon, Jamillah ^b   Sang, Bum Woo ^a   Aleshin, Alexander ^c   Choe, Jun Yong ^a  

^a ROSALIND FRANKLIN UNIVERSITY OF MEDICINE AND SCIENCE   (United States)

^b KUWAIT UNIVERSITY   (Kuwait)

^c BURNHAM INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHALASIN B; FORSKOLIN; GLUCOSE TRANSPORTER; PHLORETIN;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; GLUCOSE HOMEOSTASIS; MOLECULAR MECHANICS; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; STAPHYLOCOCCUS EPIDERMIDIS;

GLUT; MAJOR FACILITATOR SUPERFAMILY; MEMBRANE PROTEIN; SOLUTE-CARRIER 2A; SUGAR TRANSPORTER;

BIOLOGICAL TRANSPORT; CLONING, MOLECULAR; COLFORSIN; CRYSTALLIZATION; CYTOCHALASIN B; ESCHERICHIA COLI; GLUCOSE TRANSPORT PROTEINS, FACILITATIVE; HUMANS; MODELS, MOLECULAR; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; PHLORETIN; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 84887127680     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1311485110     Document Type: Article

References (50)

1. Uldry M, Thorens B (2004) The SLC2 family of facilitated hexose and polyol transporters. Pflugers Arch 447(5):480-489.
2. Thorens B, Mueckler M (2010) Glucose transporters in the 21st Century. Am J Physiol Endocrinol Metab 298(2):E141-E145.
3. Klepper J, Fischbarg J, Vera JC, Wang D, De Vivo DC (1999) GLUT1-deficiency: Barbiturates potentiate haploinsufficiency in vitro. Pediatr Res 46(6):677-683.
4. Santer R, et al. (1997) Mutations in GLUT2, the gene for the liver-Type glucose transporter, in patients with Fanconi-Bickel syndrome. Nat Genet 17(3):324-326.
5. Medina RA, Owen GI (2002) Glucose transporters: Expression, regulation and cancer. Biol Res 35(1):9-26.
6. Airley RE, Mobasheri A (2007) Hypoxic regulation of glucose transport, anaerobic metabolism and angiogenesis in cancer: Novel pathways and targets for anticancer therapeutics. Chemotherapy 53(4):233-256.
7. Watson RT, Pessin JE (2001) Intracellular organization of insulin signaling and GLUT4 translocation. Recent Prog Horm Res 56:175-193.
8. Uldry M, et al. (2001) Identification of a mammalian H(+)-myo-inositol symporter expressed predominantly in the brain. EMBO J 20(16):4467-4477.
9. Wilson-O'Brien AL, Patron N, Rogers S (2010) Evolutionary ancestry and novel functions of the mammalian glucose transporter (GLUT) family. BMC Evol Biol 10:152.
10. Kruckeberg AL, Dickinson JR (2004) Carbon metabolism. Metabolism and Molecular Physiology of Saccharomyces cerevisiae, eds Dickinson JR, Schweizer M (CRC, London), 2nd Ed, pp 42-103.
11. Williams LE, Lemoine R, Sauer N (2000) Sugar transporters in higher plants-a diversity of roles and complex regulation. Trends Plant Sci 5(7):283-290.
12. Slewinski TL (2011) Diverse functional roles of monosaccharide transporters and their homologs in vascular plants: A physiological perspective. Mol Plant 4(4):641-662.
13. Sun L, et al. (2012) Crystal structure of a bacterial homologue of glucose transporters GLUT1-4. Nature 490(7420):361-366.
14. Hirai T, et al. (2002) Three-dimensional structure of a bacterial oxalate transporter. Nat Struct Biol 9(8):597-600.
15. Abramson J, et al. (2003) Structure and mechanism of the lactose permease of Escherichia coli. Science 301(5633):610-615.
16. Huang Y, Lemieux MJ, Song J, Auer M, Wang D-N (2003) Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 301(5633):616-620.
17. Yin Y, He X, Szewczyk P, Nguyen T, Chang G (2006) Structure of the multidrug transporter EmrD from Escherichia coli. Science 312(5774):741-744.
18. Dang S, et al. (2010) Structure of a fucose transporter in an outward-open conformation. Nature 467(7316):734-738.
19. Newstead S, et al. (2011) Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2. EMBO J 30(2):417-426.
20. Zheng H, Wisedchaisri G, Gonen T (2013) Crystal structure of a nitrate/nitrite exchanger. Nature 497(7451):647-651.
21. Yan H, et al. (2013) Structure and mechanism of a nitrate transporter. Cell Rep 3(3): 716-723.
22. Smirnova I, Kasho V, Kaback HR (2011) Lactose permease and the alternating access mechanism. Biochemistry 50(45):9684-9693.
23. Quistgaard EM, Löw C, Moberg P, Trésaugues L, Nordlund P (2013) Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters. Nat Struct Mol Biol 20(6):766-768.
24. Martin GE, et al. (1994) Forskolin specifically inhibits the bacterial galactose-H+ transport protein, GalP. J Biol Chem 269(40):24870-24877.
25. Sanderson NM, Qi D, Steel A, Henderson PJ (1998) Effect of the D32N and N300F mutations on the activity of the bacterial sugar transport protein, GalP. Biochem Soc Trans 26(3):S306.
26. Konings WN, Barnes EM, Jr., Kaback HR (1971) Mechanisms of active transport in isolated membrane vesicles.
27. The coupling of reduced phenazine methosulfate to the concentrative uptake of beta-galactosides and amino acids. J Biol Chem 246(19): 5857-5861.
28. Ramos S, Schuldiner S, Kaback HR (1976) The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci USA 73(6):1892-1896.
29. Kasahara T, Kasahara M (1996) Expression of the rat GLUT1 glucose transporter in the yeast Saccharomyces cerevisiae. Biochem J 315(Pt 1):177-182.
30. Sergeant S, Kim HD (1985) Inhibition of 3-O-methylglucose transport in human erythrocytes by forskolin. J Biol Chem 260(27):14677-14682.
31. Seyfang A, DuszenkoM (1991) Specificity of glucose transport in Trypanosoma brucei. Effective inhibition by phloretin and cytochalasin B. Eur J Biochem 202(1):191-196.
32. Mueckler M, Makepeace C (2009) Model of the exofacial substrate-binding site and helical folding of the human Glut1 glucose transporter based on scanning mutagenesis. Biochemistry 48(25):5934-5942.
33. Hashiramoto M, et al. (1992) Site-directed mutagenesis of GLUT1 in helix 7 residue 282 results in perturbation of exofacial ligand binding. J Biol Chem267(25):17502-17507.
34. Mueckler M, Weng W, Kruse M (1994) Glutamine 161 of Glut1 glucose transporter is critical for transport activity and exofacial ligand binding. J Biol Chem 269(32): 20533-20538.
35. Carrasco N, et al. (1989) Characterization of site-directed mutants in the lac permease of Escherichia coli.
36. Glutamate-325 replacements. Biochemistry 28(6):2533-2539.
37. Bloch R (1973) Inhibition of glucose transport in the human erythrocyte by cytochalasin B. Biochemistry 12(23):4799-4801.
38. Arechaga I, et al. (2000) Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F(1)F(o) ATP synthase. FEBS Lett 482(3):215-219.
39. Sheldrick GM (2010) Experimental phasing with SHELXC/D/E: Combining chain tracing with density modification. Acta Crystallogr D Biol Crystallogr 66(Pt 4):479-485.
40. Emsley P, Lohkamp B, Scott WG, Cowtan K (2010) Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66(Pt 4):486-501.
41. McRee DE, Israel M (2008) XtalView, protein structure solution and protein graphics, a short history. J Struct Biol 163(3):208-213.
42. Adams PD, Mustyakimov M, Afonine PV, Langan P (2009) Generalized X-ray and neutron crystallographic analysis: More accurate and complete structures for biological macromolecules. Acta Crystallogr D Biol Crystallogr 65(Pt 6):567-573.
43. Murshudov GN, et al. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr 67(Pt 4):355-367.
44. Kaback HR (1971) Bacterial membranes. Methods Enzymol 22:99-120.
45. Short SA, Kaback HR, Kohn LD (1975) Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. J Biol Chem 250(11): 4291-4296.
46. Kaczorowski GJ, Kaback HR (1979) Mechanism of lactose translocation in membrane vesicles from Escherichia coli.
47. Effect of pH on efflux, exchange, and counterflow. Biochemistry 18(17):3691-3697.
48. Kaback HR, Barnes EM, Jr. (1971) Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli. J Biol Chem 246(17):5523-5531.
49. Ramos S, Kaback HR (1977) The electrochemical proton gradient in Escherichia coli membrane vesicles. Biochemistry 16(5):848-854.
50. Geertsma ER, Nik Mahmood NAB, Schuurman-Wolters GK, Poolman B (2008) Membrane reconstitution of ABC transporters and assays of translocator function. Nat Protoc 3(2):256-266.