메뉴 건너뛰기




Volumn 32, Issue 21, 2013, Pages 2804-2818

RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core

Author keywords

3 way junction; RNA modelling; RNA structure; spliceosome; U2 U6 duplex

Indexed keywords

MESSENGER RNA PRECURSOR; NUCLEOTIDE; PSORALEN; RIBONUCLEASE H; SMALL NUCLEAR RNA;

EID: 84887115772     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.1038/emboj.2013.198     Document Type: Article
Times cited : (49)

References (82)
  • 3
    • 0035047218 scopus 로고    scopus 로고
    • Crystal structure of a model branchpoint-U2 snRNA duplex containing bulged adenosines
    • DOI 10.1017/S1355838201002187
    • Berglund JA, Rosbash M, Schultz SC (2001) Crystal structure of a model branchpoint-U2 snRNA duplex containing bulged adenosines. RNA 7: 682-691 (Pubitemid 32405865)
    • (2001) RNA , vol.7 , Issue.5 , pp. 682-691
    • Berglund, J.A.1    Rosbash, M.2    Schultz, S.C.3
  • 4
    • 78649656777 scopus 로고    scopus 로고
    • Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis
    • Bessonov S, Anokhina M, Krasauskas A, Golas MM, Sander B, Will CL, Urlaub H, Stark H, Lührmann R (2010) Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis. RNA 16: 2384-2403
    • (2010) RNA , vol.16 , pp. 2384-2403
    • Bessonov, S.1    Anokhina, M.2    Krasauskas, A.3    Golas, M.M.4    Sander, B.5    Will, C.L.6    Urlaub, H.7    Stark, H.8    Lührmann, R.9
  • 5
    • 42249093677 scopus 로고    scopus 로고
    • Isolation of an active step I spliceosome and composition of its RNP core
    • DOI 10.1038/nature06842, PII NATURE06842
    • Bessonov S, Anokhina M, Will CL, Urlaub H, Lührmann R (2008) Isolation of an active step I spliceosome and composition of its RNP core. Nature 452: 846-850 (Pubitemid 351550864)
    • (2008) Nature , vol.452 , Issue.7189 , pp. 846-850
    • Bessonov, S.1    Anokhina, M.2    Will, C.L.3    Urlaub, H.4    Luhrmann, R.5
  • 7
    • 0017757667 scopus 로고
    • 30-Phosphatase activity in T4 polynucleotide kinase
    • Cameron V, Uhlenbeck OC (1977) 30-Phosphatase activity in T4 polynucleotide kinase. Biochemistry 16: 5120-5126
    • (1977) Biochemistry , vol.16 , pp. 5120-5126
    • Cameron, V.1    Uhlenbeck, O.C.2
  • 8
    • 0022552726 scopus 로고
    • The generality of self-splicing RNA: Relationship to nuclear mRNA splicing
    • Cech TR (1986) The generality of self-splicing RNA: relationship to nuclear mRNA splicing. Cell 44: 207-210 (Pubitemid 16048206)
    • (1986) Cell , vol.44 , Issue.2 , pp. 207-210
    • Cech, T.R.1
  • 10
    • 0028569768 scopus 로고
    • Catalytic site components common to both splicing steps of a group II intron
    • Chanfreau G, Jacquier A (1994) Catalytic site components common to both splicing steps of a group II intron. Science 266: 1383-1387
    • (1994) Science , vol.266 , pp. 1383-1387
    • Chanfreau, G.1    Jacquier, A.2
  • 11
    • 0033636518 scopus 로고    scopus 로고
    • Functional association of U2 snRNP with the ATP-independent spliceosomal complex e
    • Das R, Zhou Z, Reed R (2000) Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell 5: 779-787
    • (2000) Mol Cell , vol.5 , pp. 779-787
    • Das, R.1    Zhou, Z.2    Reed, R.3
  • 12
    • 33745860086 scopus 로고    scopus 로고
    • Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions
    • DOI 10.1128/MCB.00582-06
    • Deckert J, Hartmuth K, Boehringer D, Behzadnia N, Will CL, Kastner B, Stark H, Urlaub H, Lührmann R (2006) Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions. Mol Cell Biol 26: 5528-5543 (Pubitemid 44036218)
    • (2006) Molecular and Cellular Biology , vol.26 , Issue.14 , pp. 5528-5543
    • Deckert, J.1    Hartmuth, K.2    Boehringer, D.3    Behzadnia, N.4    Will, C.L.5    Kastner, B.6    Stark, H.7    Urlaub, H.8    Luhrmann, R.9
  • 13
    • 0031719371 scopus 로고    scopus 로고
    • Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae
    • Dix I, Russell CS, O'Keefe RT, Newman AJ, Beggs JD (1998) Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA 4: 1675-1686
    • (1998) RNA , vol.4 , pp. 1675-1686
    • Dix, I.1    Russell, C.S.2    O'Keefe, R.T.3    Newman, A.J.4    Beggs, J.D.5
  • 15
    • 0019332910 scopus 로고
    • The nucleotide sequence of nuclear U6 (4.7 S) RNA
    • Epstein P, Reddy R, Henning D, Busch H (1980) The nucleotide sequence of nuclear U6 (4.7 S) RNA. J Biol Chem 255: 8901-8906
    • (1980) J Biol Chem , vol.255 , pp. 8901-8906
    • Epstein, P.1    Reddy, R.2    Henning, D.3    Busch, H.4
  • 16
    • 0026654005 scopus 로고
    • Thiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicing in vitro
    • Fabrizio P, Abelson J (1992) Thiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicing in vitro. Nucleic Acids Res 20: 3659-3664
    • (1992) Nucleic Acids Res , vol.20 , pp. 3659-3664
    • Fabrizio, P.1    Abelson, J.2
  • 17
    • 70449625597 scopus 로고    scopus 로고
    • The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome
    • Fabrizio P, Dannenberg J, Dube P, Kastner B, Stark H, Urlaub H, Lührmann R (2009) The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome. Mol Cell 36: 593-608
    • (2009) Mol Cell , vol.36 , pp. 593-608
    • Fabrizio, P.1    Dannenberg, J.2    Dube, P.3    Kastner, B.4    Stark, H.5    Urlaub, H.6    Lührmann, R.7
  • 18
    • 0028144641 scopus 로고
    • A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing
    • Fortner DM, Troy RG, Brow DA (1994) A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing. Genes Dev 8: 221-233
    • (1994) Genes Dev , vol.8 , pp. 221-233
    • Fortner, D.M.1    Troy, R.G.2    Brow, D.A.3
  • 19
    • 0033119176 scopus 로고    scopus 로고
    • Initial recognition of U12-dependent introns requires both U11/5' splice-site and U12/branchpoint interactions
    • Frilander MJ, Steitz JA (1999) Initial recognition of U12-dependent introns requires both U11/50 splice-site and U12/branchpoint interactions. Genes Dev 13: 851-863 (Pubitemid 29169844)
    • (1999) Genes and Development , vol.13 , Issue.7 , pp. 851-863
    • Frilander, M.J.1    Steitz, J.A.2
  • 20
    • 84873629024 scopus 로고    scopus 로고
    • Crystal structure of Prp8 reveals active site cavity of the spliceosome
    • Galej WP, Oubridge C, Newman AJ, Nagai K (2013) Crystal structure of Prp8 reveals active site cavity of the spliceosome. Nature 493: 638-643
    • (2013) Nature , vol.493 , pp. 638-643
    • Galej, W.P.1    Oubridge, C.2    Newman, A.J.3    Nagai, K.4
  • 22
    • 0030044836 scopus 로고    scopus 로고
    • Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A
    • Gozani O, Feld R, Reed R (1996) Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A. Genes Dev 10: 233-243 (Pubitemid 26044610)
    • (1996) Genes and Development , vol.10 , Issue.2 , pp. 233-243
    • Gozani, O.1    Feld, R.2    Reed, R.3
  • 23
    • 0033534531 scopus 로고    scopus 로고
    • An unusual chemical reactivity of Sm site adenosines strongly correlates with proper assembly of core U snRNP particles
    • DOI 10.1006/jmbi.1998.2300
    • Hartmuth K, Raker VA, Huber J, Branlant C, Lührmann R (1999) An unusual chemical reactivity of Sm site adenosines strongly correlates with proper assembly of core U snRNP particles. J Mol Biol 285: 133-147 (Pubitemid 29034032)
    • (1999) Journal of Molecular Biology , vol.285 , Issue.1 , pp. 133-147
    • Hartmuth, K.1    Raker, V.A.2    Huber, J.3    Branlant, C.4    Luhrmann, R.5
  • 24
    • 84885522212 scopus 로고    scopus 로고
    • Assembly and isolation of spliceosomal complexes in vitro
    • Stamm S, Smith C, Lührmann R (eds), 1st edn Weinheim, Germany: Wiley-VCH
    • Hartmuth K, van Santen MA, Odenwälder P, Lürhmann R (2012) Assembly and isolation of spliceosomal complexes in vitro. In Alternative pre-mRNA Splicing: Theory and Protocols, Stamm S, Smith C, Lührmann R (eds), 1st edn, pp 331-340. Weinheim, Germany: Wiley-VCH
    • (2012) Alternative Pre-mRNA Splicing: Theory and Protocols , pp. 331-340
    • Hartmuth, K.1    Van Santen, M.A.2    Odenwälder, P.3    Lürhmann, R.4
  • 25
    • 0025639012 scopus 로고
    • Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles
    • Hausner TP, Giglio LM, Weiner AM (1990) Evidence for basepairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles. Genes Dev 4: 2146-2156 (Pubitemid 120034521)
    • (1990) Genes and Development , vol.4 , Issue.12 , pp. 2146-2156
    • Hausner, T.-P.1    Giglio, L.M.2    Weiner, A.M.3
  • 26
    • 34147179830 scopus 로고    scopus 로고
    • U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing
    • DOI 10.1101/gad.1536107
    • Hilliker AK, Mefford MA, Staley JP (2007) U2 toggles iteratively between the stem IIa and stem IIc conformations to promote premRNA splicing. Genes Dev 21: 821-834 (Pubitemid 46572361)
    • (2007) Genes and Development , vol.21 , Issue.7 , pp. 821-834
    • Hilliker, A.K.1    Mefford, M.A.2    Staley, J.P.3
  • 27
    • 2442661481 scopus 로고    scopus 로고
    • Multiple functions for the invariant AGC triad of U6 snRNA
    • DOI 10.1261/rna.7310704
    • Hilliker AK, Staley JP (2004) Multiple functions for the invariant AGC triad of U6 snRNA. RNA 10: 921-928 (Pubitemid 38669765)
    • (2004) RNA , vol.10 , Issue.6 , pp. 921-928
    • Hilliker, A.K.1    Staley, J.P.2
  • 28
    • 77955405123 scopus 로고    scopus 로고
    • Assemble: An interactive graphical tool to analyze and build RNA architectures at the 2D and 3D levels
    • Jossinet F, Ludwig TE, Westhof E (2010) Assemble: an interactive graphical tool to analyze and build RNA architectures at the 2D and 3D levels. Bioinformatics 26: 2057-2059
    • (2010) Bioinformatics , vol.26 , pp. 2057-2059
    • Jossinet, F.1    Ludwig, T.E.2    Westhof, E.3
  • 29
    • 75649116868 scopus 로고    scopus 로고
    • A structural analysis of the group II intron active site and implications for the spliceosome
    • Keating KS, Toor N, Perlman PS, Pyle AM (2010) A structural analysis of the group II intron active site and implications for the spliceosome. RNA 16: 1-9
    • (2010) RNA , vol.16 , pp. 1-9
    • Keating, K.S.1    Toor, N.2    Perlman, P.S.3    Pyle, A.M.4
  • 30
    • 0029965552 scopus 로고    scopus 로고
    • Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing
    • Kim SH, Lin RJ (1996) Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol Cell Biol 16: 6810-6819
    • (1996) Mol Cell Biol , vol.16 , pp. 6810-6819
    • Kim, S.H.1    Lin, R.J.2
  • 31
    • 77149180424 scopus 로고    scopus 로고
    • Release of SF3 from the intron branchpoint activates the first step of premRNA splicing
    • Lardelli RM, Thompson JX, Yates 3rd JR, Stevens SW (2010) Release of SF3 from the intron branchpoint activates the first step of premRNA splicing. RNA 16: 516-528
    • (2010) RNA , vol.16 , pp. 516-528
    • Lardelli, R.M.1    Thompson, J.X.2    Yates III, J.R.3    Stevens, S.W.4
  • 32
    • 0027739852 scopus 로고
    • Mutations in U6 snRNA that alter splice site specificity: Implications for the active site
    • Lesser CF, Guthrie C (1993) Mutations in U6 snRNA that alter splice site specificity: implications for the active site. Science 262: 1982-1988 (Pubitemid 24041875)
    • (1993) Science , vol.262 , Issue.5142 , pp. 1982-1988
    • Lesser, C.F.1    Guthrie, C.2
  • 33
    • 84858800333 scopus 로고    scopus 로고
    • Structure and assembly of the SF3a splicing factor complex of U2 snRNP
    • Lin PC, Xu RM (2012) Structure and assembly of the SF3a splicing factor complex of U2 snRNP. EMBO J 31: 1579-1590
    • (2012) EMBO J , vol.31 , pp. 1579-1590
    • Lin, P.C.1    Xu, R.M.2
  • 34
    • 0030694848 scopus 로고    scopus 로고
    • Crosslinking of the U5 snRNP-specific 116-kDa protein to RNA hairpins that block step 2 of splicing
    • Liu ZR, Laggerbauer B, Lührmann R, Smith CW (1997) Crosslinking of the U5 snRNP-specific 116-kDa protein to RNA hairpins that block step 2 of splicing. RNA 3: 1207-1219 (Pubitemid 27495094)
    • (1997) RNA , vol.3 , Issue.11 , pp. 1207-1219
    • Liu, Z.-R.1    Laggerbauer, B.2    Luhrmann, R.3    Smith, C.W.J.4
  • 35
    • 0026486883 scopus 로고
    • A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome
    • Madhani HD, Guthrie C (1992) A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 71: 803-817
    • (1992) Cell , vol.71 , pp. 803-817
    • Madhani, H.D.1    Guthrie, C.2
  • 37
    • 0035796398 scopus 로고    scopus 로고
    • Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing
    • DOI 10.1093/emboj/20.13.3577
    • McConnell TS, Steitz JA (2001) Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing. EMBO J 20: 3577-3586 (Pubitemid 32634364)
    • (2001) EMBO Journal , vol.20 , Issue.13 , pp. 3577-3586
    • McConnell, T.S.1    Steitz, J.A.2
  • 38
    • 0027215924 scopus 로고
    • A functional association between the 5' and 3' splice sites is established in the earliest prespliceosome complex (E) in mammals
    • Michaud S, Reed R (1993) A functional association between the 50 and 30 splice site is established in the earliest prespliceosome complex (E) in mammals. Genes Dev 7: 1008-1020 (Pubitemid 23170825)
    • (1993) Genes and Development , vol.7 , Issue.6 , pp. 1008-1020
    • Michaud, S.1    Reed, R.2
  • 39
    • 63549105637 scopus 로고    scopus 로고
    • The ribozyme core of group II introns: A structure in want of partners
    • Michel F, Costa M, Westhof E (2009) The ribozyme core of group II introns: a structure in want of partners. Trends Biochem Sci 34: 189-199
    • (2009) Trends Biochem Sci , vol.34 , pp. 189-199
    • Michel, F.1    Costa, M.2    Westhof, E.3
  • 40
    • 0027364421 scopus 로고
    • Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing
    • DOI 10.1038/365364a0
    • Moore MJ, Sharp PA (1993) Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing. Nature 365: 364-368 (Pubitemid 23288982)
    • (1993) Nature , vol.365 , Issue.6444 , pp. 364-368
    • Moore, M.J.1    Sharp, P.A.2
  • 41
    • 0026605043 scopus 로고
    • U5 snRNA interacts with exon sequences at 50 and 30 splice sites
    • Newman AJ, Norman C (1992) U5 snRNA interacts with exon sequences at 50 and 30 splice sites. Cell 68: 743-754
    • (1992) Cell , vol.68 , pp. 743-754
    • Newman, A.J.1    Norman, C.2
  • 42
    • 21244451435 scopus 로고    scopus 로고
    • Crystal structures of RNase H bound to an RNA/DNA hybrid: Substrate specificity and metal-dependent catalysis
    • DOI 10.1016/j.cell.2005.04.024, PII S0092867405004046
    • Nowotny M, Gaidamakov SA, Crouch RJ, Yang W (2005) Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell 121: 1005-1016 (Pubitemid 40884393)
    • (2005) Cell , vol.121 , Issue.7 , pp. 1005-1016
    • Nowotny, M.1    Gaidamakov, S.A.2    Crouch, R.J.3    Yang, W.4
  • 43
    • 0032518905 scopus 로고    scopus 로고
    • Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing
    • DOI 10.1093/emboj/17.2.565
    • O'Keefe RT, Newman AJ (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17: 565-574 (Pubitemid 28045496)
    • (1998) EMBO Journal , vol.17 , Issue.2 , pp. 565-574
    • O'Keefe, R.T.1    Newman, A.J.2
  • 44
    • 0030598839 scopus 로고    scopus 로고
    • The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast
    • DOI 10.1016/S0092-8674(00)80140-3
    • O'Keefe RT, Norman C, Newman AJ (1996) The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of premRNA splicing in yeast. Cell 86: 679-689 (Pubitemid 26299498)
    • (1996) Cell , vol.86 , Issue.4 , pp. 679-689
    • O'Keefe, R.T.1    Norman, C.2    Newman, A.J.3
  • 46
    • 0028769660 scopus 로고
    • The stereochemical course of group II intron self-splicing
    • Padgett RA, Podar M, Boulanger SC, Perlman PS (1994) The stereochemical course of group II intron self-splicing. Science 266: 1685-1688
    • (1994) Science , vol.266 , pp. 1685-1688
    • Padgett, R.A.1    Podar, M.2    Boulanger, S.C.3    Perlman, P.S.4
  • 47
    • 34147174235 scopus 로고    scopus 로고
    • Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing
    • DOI 10.1101/gad.1524307
    • Perriman RJ, Ares Jr M (2007) Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing. Genes Dev 21: 811-820 (Pubitemid 46572360)
    • (2007) Genes and Development , vol.21 , Issue.7 , pp. 811-820
    • Perriman, R.J.1    Ares Jr., M.2
  • 48
    • 84858799296 scopus 로고    scopus 로고
    • Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre
    • Rasche N, Dybkov O, Schmitzova J, Akyildiz B, Fabrizio P, Lührmann R (2012) Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre. EMBO J 31: 1591-1604
    • (2012) EMBO J , vol.31 , pp. 1591-1604
    • Rasche, N.1    Dybkov, O.2    Schmitzova, J.3    Akyildiz, B.4    Fabrizio, P.5    Lührmann, R.6
  • 49
    • 0033053502 scopus 로고    scopus 로고
    • The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5' splice site
    • DOI 10.1017/S1355838299981785
    • Reyes JL, Gustafson EH, Luo HR, Moore MJ, Konarska MM (1999) The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 50 splice site. RNA 5: 167-179 (Pubitemid 29079277)
    • (1999) RNA , vol.5 , Issue.2 , pp. 167-179
    • Reyes, J.L.1    Gustafson, E.H.2    Hongbo R, L.3    Moore, M.J.4    Konarska, M.M.5
  • 50
    • 33745755054 scopus 로고    scopus 로고
    • Proximity of conserved U6 and U2 snRNA elements to the 5′ splice site region in activated spliceosomes
    • DOI 10.1038/sj.emboj.7601134, PII 7601134
    • Rhode BM, Hartmuth K, Westhof E, Lührmann R (2006) Proximity of conserved U6 and U2 snRNA elements to the 50 splice site region in activated spliceosomes. EMBO J 25: 2475-2486 (Pubitemid 44012230)
    • (2006) EMBO Journal , vol.25 , Issue.11 , pp. 2475-2486
    • Rhode, B.M.1    Hartmuth, K.2    Westhof, E.3    Luhrmann, R.4
  • 51
    • 0034193510 scopus 로고    scopus 로고
    • Protein docking using spherical polar Fourier correlations
    • DOI 10.1002/(SICI)1097-0134(20000501)39:2<178::AID-PROT8>3.0.CO;2-6
    • Ritchie DW, Kemp GJ (2000) Protein docking using spherical polar Fourier correlations. Proteins 39: 178-194 (Pubitemid 30176485)
    • (2000) Proteins: Structure, Function and Genetics , vol.39 , Issue.2 , pp. 178-194
    • Ritchie, D.W.1    Kemp, G.J.L.2
  • 52
    • 0022424110 scopus 로고
    • An RNA processing activity that debranches RNA lariats
    • Ruskin B, Green MR (1985) An RNA processing activity that debranches RNA lariats. Science 229: 135-140
    • (1985) Science , vol.229 , pp. 135-140
    • Ruskin, B.1    Green, M.R.2
  • 53
    • 0021719524 scopus 로고
    • Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro
    • Ruskin B, Krainer AR, Maniatis T, Green MR (1984) Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro. Cell 38: 317-331 (Pubitemid 15216559)
    • (1984) Cell , vol.38 , Issue.1 , pp. 317-331
    • Ruskin, B.1    Krainer, A.R.2    Maniatis, T.3    Green, M.R.4
  • 54
    • 0029814112 scopus 로고    scopus 로고
    • RNA photolabeling mechanistic studies: X-ray crystal structure of the photoproduct formed between 4-thiothymidine and adenosine upon near UV irradiation
    • DOI 10.1021/ja961329g
    • Saintomé C, Clivio P, Favre A, Fourrey J-L, Riche C (1996) RNA photolabeling mechanistic studies: X-ray crystal structure of the photoproduct formed between 4-thiothymidine and adenosine upon near UV irradiation. J Am Chem Soc 118: 8142-8143 (Pubitemid 26305177)
    • (1996) Journal of the American Chemical Society , vol.118 , Issue.34 , pp. 8142-8143
    • Saintome, C.1    Clivio, P.2    Favre, A.3    Fourrey, J.-L.4    Riche, C.5
  • 58
    • 0345003719 scopus 로고    scopus 로고
    • Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts
    • Ségault V, Will CL, Polycarpou-Schwarz M, Mattaj IW, Branlant C, Lührmann R (1999) Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts. Mol Cell Biol 19: 2782-2790 (Pubitemid 29144522)
    • (1999) Molecular and Cellular Biology , vol.19 , Issue.4 , pp. 2782-2790
    • Segault, V.1    Will, C.L.2    Polycarpou-Schwarz, M.3    Mattaj, I.W.4    Branlant, C.5    Luhrmann, R.6
  • 59
    • 0022131865 scopus 로고
    • On the origin of RNA splicing and introns
    • Sharp PA (1985) On the origin of RNA splicing and introns. Cell 42: 397-400
    • (1985) Cell , vol.42 , pp. 397-400
    • Sharp, P.A.1
  • 61
    • 0036285645 scopus 로고    scopus 로고
    • A catalytically active group II intron domain 5 can function in the U12-dependent spliceosome
    • DOI 10.1016/S1097-2765(02)00505-1
    • Shukla GC, Padgett RA (2002) A catalytically active group II intron domain 5 can function in the U12-dependent spliceosome. Mol Cell 9: 1145-1150 (Pubitemid 34626682)
    • (2002) Molecular Cell , vol.9 , Issue.5 , pp. 1145-1150
    • Shukla, G.C.1    Padgett, R.A.2
  • 62
    • 0033179750 scopus 로고    scopus 로고
    • Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center
    • Siatecka M, Reyes JL, Konarska MM (1999) Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center. Genes Dev 13: 1983-1993 (Pubitemid 29407365)
    • (1999) Genes and Development , vol.13 , Issue.15 , pp. 1983-1993
    • Siatecka, M.1    Reyes, J.L.2    Konarska, M.M.3
  • 63
    • 0024470631 scopus 로고
    • Scanning from an independently specified branch point defines the 3' splice site of mammalian introns
    • DOI 10.1038/342243a0
    • Smith CW, Porro EB, Patton JG, Nadal-Ginard B (1989) Scanning from an independently specified branch point defines the 30 splice site of mammalian introns. Nature 342: 243-247 (Pubitemid 19283319)
    • (1989) Nature , vol.342 , Issue.6247 , pp. 243-247
    • Smith, C.W.J.1    Porro, E.B.2    Patton, J.G.3    Nadal-Ginard, B.4
  • 64
    • 58149088299 scopus 로고    scopus 로고
    • A critical assessment of the utility of protein-free splicing systems
    • Smith DJ, Konarska MM (2009) A critical assessment of the utility of protein-free splicing systems. RNA 15: 1-3
    • (2009) RNA , vol.15 , pp. 1-3
    • Smith, D.J.1    Konarska, M.M.2
  • 65
    • 0033166446 scopus 로고    scopus 로고
    • Metal ion catalysis during group II intron self-splicing: Parallels with the spliceosome
    • Sontheimer EJ, Gordon PM, Piccirilli JA (1999) Metal ion catalysis during group II intron self-splicing: parallels with the spliceosome. Genes Dev 13: 1729-1741 (Pubitemid 29325877)
    • (1999) Genes and Development , vol.13 , Issue.13 , pp. 1729-1741
    • Sontheimer, E.J.1    Gordon, P.M.2    Piccirilli, J.A.3
  • 66
    • 0027739853 scopus 로고
    • The U5 and U6 small nuclear RNAs as active site components of the spliceosome
    • Sontheimer EJ, Steitz JA (1993) The U5 and U6 small nuclear RNAs as active site components of the spliceosome. Science 262: 1989-1996 (Pubitemid 24041876)
    • (1993) Science , vol.262 , Issue.5142 , pp. 1989-1996
    • Sontheimer, E.J.1    Steitz, J.A.2
  • 67
    • 0032489021 scopus 로고    scopus 로고
    • Mechanical devices of the spliceosome: Motors, clocks, springs, and things
    • DOI 10.1016/S0092-8674(00)80925-3
    • Staley JP, Guthrie C (1998) Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell 92: 315-326 (Pubitemid 28093010)
    • (1998) Cell , vol.92 , Issue.3 , pp. 315-326
    • Staley, J.P.1    Guthrie, C.2
  • 68
    • 0033010430 scopus 로고    scopus 로고
    • An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p
    • DOI 10.1016/S1097-2765(00)80174-4
    • Staley JP, Guthrie C (1999) An RNA switch at the 50 splice site requires ATP and the DEAD box protein Prp28p. Mol Cell 3: 55-64 (Pubitemid 29292477)
    • (1999) Molecular Cell , vol.3 , Issue.1 , pp. 55-64
    • Staley, J.P.1    Guthrie, C.2
  • 70
    • 0028917214 scopus 로고
    • A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing
    • Sun JS, Manley JL (1995) A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing. Genes Dev 9: 843-854
    • (1995) Genes Dev , vol.9 , pp. 843-854
    • Sun, J.S.1    Manley, J.L.2
  • 71
    • 0028158655 scopus 로고
    • The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA
    • Teigelkamp S, McGarvey M, Plumpton M, Beggs JD (1994) The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA. EMBO J 13: 888-897 (Pubitemid 24057520)
    • (1994) EMBO Journal , vol.13 , Issue.4 , pp. 888-897
    • Teigelkamp, S.1    McGarvey, M.2    Plumpton, M.3    Beggs, J.D.4
  • 72
    • 41749109563 scopus 로고    scopus 로고
    • Crystal structure of a self-spliced group II intron
    • DOI 10.1126/science.1153803
    • Toor N, Keating KS, Taylor SD, Pyle AM (2008) Crystal structure of a self-spliced group II intron. Science 320: 77-82 (Pubitemid 351490756)
    • (2008) Science , vol.320 , Issue.5872 , pp. 77-82
    • Toor, N.1    Keating, K.S.2    Taylor, S.D.3    Pyle, A.M.4
  • 73
    • 33344460290 scopus 로고    scopus 로고
    • Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome
    • DOI 10.1261/rna.2229706
    • Turner IA, Norman CM, Churcher MJ, Newman AJ (2006) Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome. RNA 12: 375-386 (Pubitemid 43290866)
    • (2006) RNA , vol.12 , Issue.3 , pp. 375-386
    • Turner, I.A.1    Norman, C.M.2    Churcher, M.J.3    Newman, A.J.4
  • 74
    • 68349106854 scopus 로고    scopus 로고
    • Minimumenergy path for a U6 RNA conformational change involving protonation, base-pair rearrangement and base flipping
    • Venditti V, Clos 2nd L, Niccolai N, Butcher SE (2009) Minimumenergy path for a U6 RNA conformational change involving protonation, base-pair rearrangement and base flipping. J Mol Biol 391: 894-905
    • (2009) J Mol Biol , vol.391 , pp. 894-905
    • Venditti, V.1    Clos II, L.2    Niccolai, N.3    Butcher, S.E.4
  • 75
    • 60349104299 scopus 로고    scopus 로고
    • The spliceosome: Design principles of a dynamic RNP machine
    • Wahl MC, Will CL, Lührmann R (2009) The spliceosome: design principles of a dynamic RNP machine. Cell 136: 701-718
    • (2009) Cell , vol.136 , pp. 701-718
    • Wahl, M.C.1    Will, C.L.2    Lührmann, R.3
  • 76
    • 77956495754 scopus 로고    scopus 로고
    • Inclusion of weak high-resolution X-ray data for improvement of a group II intron structure
    • Wang J (2010) Inclusion of weak high-resolution X-ray data for improvement of a group II intron structure. Acta Crystallogr D Biol Crystallogr 66: 988-1000
    • (2010) Acta Crystallogr D Biol Crystallogr , vol.66 , pp. 988-1000
    • Wang, J.1
  • 78
    • 0026670431 scopus 로고
    • Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing
    • Wassarman DA, Steitz JA (1992) Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing. Science 257: 1918-1925
    • (1992) Science , vol.257 , pp. 1918-1925
    • Wassarman, D.A.1    Steitz, J.A.2
  • 79
    • 0021879256 scopus 로고
    • Crystallographic refinement of yeast aspartic acid transfer RNA
    • Westhof E, Dumas P, Moras D (1985) Crystallographic refinement of yeast aspartic acid transfer RNA. J Mol Biol 184: 119-145
    • (1985) J Mol Biol , vol.184 , pp. 119-145
    • Westhof, E.1    Dumas, P.2    Moras, D.3
  • 81
    • 0030039512 scopus 로고    scopus 로고
    • Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits
    • Yan D, Ares Jr M (1996) Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol Cell Biol 16: 818-828 (Pubitemid 26061651)
    • (1996) Molecular and Cellular Biology , vol.16 , Issue.3 , pp. 818-828
    • Yan, D.1    Ares Jr., M.2
  • 82
    • 0034649666 scopus 로고    scopus 로고
    • Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome
    • DOI 10.1038/35048617
    • Yean SL, Wuenschell G, Termini J, Lin RJ (2000) Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome. Nature 408: 881-884 (Pubitemid 32016101)
    • (2000) Nature , vol.408 , Issue.6814 , pp. 881-884
    • Yean, S.-L.1    Wuenschell, G.2    Termini, J.3    Lin, R.-J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.