메뉴 건너뛰기




Volumn 9, Issue 45, 2013, Pages 10885-10890

Motility fractionation of bacteria by centrifugation

Author keywords

[No Author keywords available]

Indexed keywords

CENTRIFUGATION; DIFFUSION;

EID: 84887096795     PISSN: 1744683X     EISSN: 17446848     Source Type: Journal    
DOI: 10.1039/c3sm51223a     Document Type: Article
Times cited : (42)

References (34)
  • 26
    • 84956525970 scopus 로고    scopus 로고
    • (SPIE Tutorial Text TT88) (SPIE Tutorial Texts) Following ref. 35 we approximate the cell body with a prolate ellipsoid and the flagellar bundle 34 as a helix obtaining a mobility μ ≃ 30 μm s-1 pN -1. The centrifugal force f acting on one single bacterium is f = Δma where a is the centrifugal acceleration and Δm is the buoyant mass of the cell that can be expressed as Δm = ΔρV, Δρ being the excess density of the cell with respect to the density of the medium, and V being the volume of the cell. By taking the Δρ measured in ref. 36 we can estimate νd ≃ 1 μm s-1 at ν = 10 Hz
    • G. H. Seward, Optical Design of Microscopes, (SPIE Tutorial Text Vol. TT88) (SPIE Tutorial Texts)
    • Optical Design of Microscopes
    • Seward, G.H.1
  • 27
    • 15544387869 scopus 로고    scopus 로고
    • Springer-Verlag, New York, Note that the measurements are taken after about one minute from the stop of the centrifuge. If we assume that the reorientation of the bacteria is a random event occurring at a constant rate λ the fraction of bacteria that have not tumbled from at the beginning of the measurement is only a few percents for λ as low as 0.05 Hz. This can be easily estimated from the Poisson distribution
    • H. C. Berg, E. coli in Motion, Springer-Verlag, New York, 2004
    • (2004) E. Coli in Motion
    • Berg, H.C.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.