The viral polymerase inhibitor 2'-c-methylcytidine inhibits norwalk virus replication and protects against norovirus-induced diarrhea and mortality in a mouse model

Journal of Virology

Volumn 87, Issue 21, 2013, Pages 11798-11805

  Rocha Pereira, Joana ^a   Jochmans, Dirk ^b   Debing, Yannick ^b   Verbeken, Erik ^c   Nascimento, Maria S J ^a   Neyts, Johan ^b  

^a UNIVERSITY OF PORTO   (Portugal)

^b REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

^c DEPARTMENT OF IMAGING AND PATHOLOGY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

2' METHYLCYTIDINE; SODIUM CHLORIDE; VIRUS RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIVIRAL ACTIVITY; ARTICLE; BODY WEIGHT; CONTROLLED STUDY; DIARRHEA; DISEASE MODEL; FECES ANALYSIS; FEMALE; HISTOPATHOLOGY; HUMAN; HUMAN CELL; INFECTION RESISTANCE; INTESTINE; LIMIT OF DETECTION; LUNG PARENCHYMA; MALE; MESENTERY LYMPH NODE; MORTALITY; MOUSE; NONHUMAN; NOROVIRUS; NOROVIRUS INFECTION; PRIORITY JOURNAL; PROVOCATION; QUANTITATIVE ANALYSIS; REPLICON; SPLEEN; SURVIVAL RATE; VIRUS INHIBITION; VIRUS LOAD; VIRUS REPLICATION;

ANIMAL STRUCTURES; ANIMALS; ANTIVIRAL AGENTS; BODY WEIGHT; CALICIVIRIDAE INFECTIONS; CYTIDINE; DIARRHEA; DISEASE MODELS, ANIMAL; FECES; FEMALE; MALE; MICE; NOROVIRUS; NORWALK VIRUS; RNA, VIRAL; SURVIVAL ANALYSIS; VIRAL LOAD; VIRUS REPLICATION;

EID: 84886924040     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.02064-13     Document Type: Article

References (29)

1. Patel MM, Widdowson MA, Glass RI, Akazawa K, Vinje J, Parashar UD. 2008. Systematic literature review of role of noroviruses in sporadic gastroenteritis. Emerg. Infect. Dis. 14:1224-1231.
2. Hemming M, Rasanen S, Huhti L, Paloniemi M, Salminen M, Vesikari T. 2013. Major reduction of rotavirus, but not norovirus, gastroenteritis in children seen in hospital after the introduction of RotaTeq vaccine into the National Immunization Programme in Finland. Eur. J. Pediatr. 172: 739-746.
3. Payne DC, Vinje J, Szilagyi PG, Edwards KM, Staat MA, Weinberg GA, Hall CB, Chappell J, Bernstein DI, Curns AT, Wikswo M, Shirley SH, Hall AJ, Lopman B, Parashar UD. 2013. Norovirus and medically attended gastroenteritis in U.S. children. N. Engl. J. Med. 368:1121-1130.
4. Bok K, Green KY. 2012. Norovirus gastroenteritis in immunocompromised patients. N. Engl. J. Med. 367:2126-2132.
5. Teunis PF, Moe CL, Liu P, Miller SE, Lindesmith L, Baric RS, Le Pendu J, Calderon RL. 2008. Norwalk virus: how infectious is it? J. Med. Virol. 80:1468-1476.
6. Atmar RL, Opekun AR, Gilger MA, Estes MK, Crawford SE, Neill FH, Graham DY. 2008. Norwalk virus shedding after experimental human infection. Emerg. Infect. Dis. 14:1553-1557.
7. Siebenga JJ, Beersma MF, Vennema H, van Biezen P, Hartwig NJ, Koopmans M. 2008. High prevalence of prolonged norovirus shedding and illness among hospitalized patients: a model for in vivo molecular evolution. J. Infect. Dis. 198:994-1001.
8. Lopman B, Gastanaduy P, Park GW, Hall AJ, Parashar UD, Vinje J. Environmental transmission of norovirus gastroenteritis. Curr. Opin. Virol. 2:96-102.
9. Chang KO, Sosnovtsev SV, Belliot G, King AD, Green KY. 2006. Stableexpression of a Norwalk virus RNA replicon in a human hepatoma cell line. Virology 353:463-473.
10. Karst SM, Wobus CE, Lay M, Davidson J, Virgin HW, IV. 2003. STAT1-dependent innate immunity to a Norwalk-like virus. Science 299: 1575-1578.
11. Taube S, Kolawole AO, Hohne M, Wilkinson JE, Handley SA, Perry JW, Thackray LB, Akkina R, Wobus CE. 2013. A mouse model for human norovirus. mBio 4:e00450-00413. doi:10.1128/mBio.00450-13.
12. Wobus CE, Karst SM, Thackray LB, Chang KO, Sosnovtsev SV, Belliot G, Krug A, Mackenzie JM, Green KY, Virgin HW. 2004. Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages. PLoS Biol. 2:e432. doi:10.1371/journal.pbio.0020432.
13. Kahan SM, Liu G, Reinhard MK, Hsu CC, Livingston RS, Karst SM. Comparative murine norovirus studies reveal a lack of correlation between intestinal virus titers and enteric pathology. Virology 421:202-210.
14. Mumphrey SM, Changotra H, Moore TN, Heimann-Nichols ER, Wobus CE, Reilly MJ, Moghadamfalahi M, Shukla D, Karst SM. 2007. Murine norovirus 1 infection is associated with histopathological changes in immunocompetent hosts, but clinical disease is prevented by STAT1-dependent interferon responses. J. Virol. 81:3251-3263.
15. Strong DW, Thackray LB, Smith TJ, Virgin HW. 2012. Protruding domain of capsid protein is necessary and sufficient to determine murine norovirus replication and pathogenesis in vivo. J. Virol. 86:2950-2958.
16. Johnson AJ, Roehrig JT. 1999. New mouse model for dengue virus vaccine testing. J. Virol. 73:783-786.
17. Rocha-Pereira J, Jochmans D, Dallmeier K, Leyssen P, Cunha R, Costa I, Nascimento MS, Neyts J. 2012. Inhibition of norovirus replication by the nucleoside analogue 2'-C-methylcytidine. Biochem. Biophys. Res. Commun. 427:796-800.
18. Pierra C, Amador A, Benzaria S, Cretton-Scott E, D'Amours M, Mao J, Mathieu S, Moussa A, Bridges EG, Standring DN, Sommadossi JP, Storer R, Gosselin G. 2006. Synthesis and pharmacokinetics of valopicitabine (NM283), an efficient prodrug of the potent anti-HCV agent 2'- C-methylcytidine. J. Med. Chem. 49:6614-6620.
19. PfafflMW. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45. doi:10.1093/nar/29.9.e45.
20. Paeshuyse J, Kaul A, De Clercq E, Rosenwirth B, Dumont JM, Scalfaro P, Bartenschlager R, Neyts J. 2006. The non-immunosuppressive cyclosporin DEBIO-025 is a potent inhibitor of hepatitis C virus replication in vitro. Hepatology 43:761-770.
21. Spann KM, Tran KC, Chi B, Rabin RL, Collins PL. 2004. Suppression of the induction of alpha, beta, and lambda interferons by the NS1 and NS2 proteins of human respiratory syncytial virus in human epithelial cells and macrophages. J. Virol. 78:4363-4369.
22. Rocha-Pereira J, Jochmans D, Dallmeier K, Leyssen P, Nascimento MS, Neyts J. 2012. Favipiravir (T-705) inhibits in vitro norovirus replication. Biochem. Biophys. Res. Commun. 424:777-780.
23. Delang L, Vliegen I, Froeyen M, Neyts J. 2011. Comparative study of the genetic barriers and pathways towards resistance of selective inhibitors of hepatitis C virus replication. Antimicrob. Agents Chemother. 55:4103-4113.
24. Afdhal N, Brien O C, Godofsky E, Rodriguez-Torres M, Pappas SC, Pockros P, Lawitz E, Bzowej N, Rustgi V, Sulkowski M, Sherman K, Jacobson I, Chao G, Knox S, Pietropaolo K, Brown N. 2006. Valopicitabine (NM283), alone or with peginterferon, compared to peg interferon/ribavirin (pegifn/RBV) retreatment in hepatitis patients with prior nonresponse to PEGIFN/RBV: week 24 results. J. Hepatol. 44(Suppl 2): S19.
25. Afdhal N, O'Brien C, Godofsky E, Rodriguez-Torres M, Pappas SC, Lawitz E, Pockros P, Sulkowski M, Jacobson I, Chao G, Knox S, Pietropaolo K, Brown NA. 2007. Valopicitabine (nm283), alone or with peg-interferon, compared to peg interferon/ribavirin (pegifn/rbv) retreatment in patients with HCV-1 infection and prior non-response to pegifn/rbv: one-year results. J. Hepatol. 46(Suppl 1):S5.
26. Carroll SS, Tomassini JE, Bosserman M, Getty K, Stahlhut MW, Eldrup AB, Bhat B, Hall D, Simcoe AL, LaFemina R, Rutkowski CA, Wolanski B, Yang Z, Migliaccio G, De Francesco R, Kuo LC, MacCoss M, Olsen DB. 2003. Inhibition of hepatitis C virus RNA replication by 2'-modified nucleoside analogs. J. Biol. Chem. 278:11979-11984.
27. Chachu KA, LoBue AD, Strong DW, Baric RS, Virgin HW. 2008. Immune mechanisms responsible for vaccination against and clearance of mucosal and lymphatic norovirus infection. PLoS Pathog. 4:e1000236. doi:10.1371/journal.ppat.1000236.
28. Chachu KA, Strong DW, LoBue AD, Wobus CE, Baric RS, Virgin HW, IV. 2008. Antibody is critical for the clearance of murine norovirus infection. J. Virol. 82:6610-6617.
29. Lindesmith L, Moe C, Lependu J, Frelinger JA, Treanor J, Baric RS. Cellular and humoral immunity following Snow Mountain virus challenge. J. Virol. 79:2900-2909.