Gene trapping using Gal4 in zebrafish

Journal of Visualized Experiments

Volumn , Issue 79, 2013, Pages

  Balciuniene, Jorune ^a   Balciunas, Darius ^a  

^a TEMPLE UNIVERSITY   (United States)

Author keywords

Developmental biology; Gal4; Gene trap; Genetics; Genetics (animal and plant); Insertional mutagenesis; Issue 79; Mutagenesis; Transposon; Zebrafish

Indexed keywords

DNA BINDING PROTEIN; GAL VP16; GAL-VP16; GAL4 PROTEIN, ZEBRAFISH; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR; ZEBRAFISH PROTEIN;

ANIMAL; ARTICLE; FEMALE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; MALE; METHODOLOGY; ZEBRA FISH; PROCEDURES;

ANIMALS; DNA-BINDING PROTEINS; FEMALE; GENETIC VECTORS; MALE; MUTAGENESIS, INSERTIONAL; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; ZEBRAFISH; ZEBRAFISH PROTEINS;

EID: 84886850754     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50113     Document Type: Article

References (28)

1. Balciunas, D., et al. Enhancer trapping in zebrafish using the Sleeping Beauty transposon. BMC Genomics. 5 (1), 62 (2004).
2. Trinh le, A., et al. A versatile gene trap to visualize and interrogate the function of the vertebrate proteome. Genes Dev. 25 (21), 2306-2320 (2011).
3. Kawakami, K., et al. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev Cell. 7 (1), 133-144 (2004).
4. Emelyanov, A. & Parinov, S., Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Dev Biol. 320 (1), 113-121 (2008).
5. Ellingsen, S., et al. Large-scale enhancer detection in the zebrafish genome. Development. 132 (17), 3799-3811 (2005).
6. Distel, M., Wullimann, M.F., & Koster, R.W., Optimized Gal4 genetics for permanent gene expression mapping in zebrafish. Proc Natl Acad Sci U S A. 106 (32), 13365-13370 (2009).
7. Davison, J.M., et al. Transactivation from Gal4-VP16 transgenic insertions for tissue-specific cell labeling and ablation in zebrafish. Dev Biol. 304 (2), 811-824 (2007).
8. Sivasubbu, S., et al. Gene-breaking transposon mutagenesis reveals an essential role for histone H2afza in zebrafish larval development. Mech Dev. 123 (7), 513-529 (2006).
9. Clark, K.J., et al. In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Nat Methods. 8 (6), 506-512 (2011).
10. Petzold, A.M., et al. Nicotine response genetics in the zebrafish. Proc Natl Acad Sci U S A. 106 (44), 18662-18667 (2009).
11. Gaiano, N., et al. Insertional mutagenesis and rapid cloning of essential genes in zebrafish. Nature. 383 (6603), 829-832 (1996).
12. Amsterdam, A. & Hopkins, N., Mutagenesis strategies in zebrafish for identifying genes involved in development and disease. Trends Genet. 22 (9), 473-478 (2006).
13. Balciunas, D., et al. Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. PLoS Genet. 2 (11), e169 (2006).
14. Urasaki, A., Morvan, G., & Kawakami, K. Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition. Genetics. 174 (2), 639-649 (2006).
15. Haffter, P., et al. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development. 123, 1-36 (1996).
16. Amsterdam, A., et al. Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci U S A. 101 (35), 12792-12797 (2004).
17. Scheer, N. & Campos-Ortega, J.A. Use of the Gal4-UAS technique for targeted gene expression in the zebrafish. Mech Dev. 80 (2), 153-158 (1999).
18. Koster, R.W. & Fraser, S.E. Tracing transgene expression in living zebrafish embryos. Dev Biol. 233 (2), 329-346 (2001).
19. Rouet, P., Smih, F., & Jasin, M. Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease. Mol Cell Biol. 14 (12), 8096-8106 (1994).
20. Thermes, V., et al. I-SceI meganuclease mediates highly efficient transgenesis in fish. Mech Dev. 118 (1-2), 91-98 (2002).
21. Lisby, M., Mortensen, U.H., & Rothstein, R. Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre. Nat Cell Biol. 5 (6), 572-577 (2003).
22. Bennardo, N., Cheng, A., Huang, N., & Stark, J.M. Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. PLoS Genet. 4 (6), e1000110 (2008).
23. Rehn, K., Wong, K.S., Balciunas, D., & Sumanas, S. Zebrafish enhancer trap line recapitulates embryonic aquaporin 1a expression pattern in vascular endothelial cells. Int J Dev Biol. 55 (6), 613-618 (2011).
24. Petzold, A.M., et al. SCORE imaging: specimen in a corrected optical rotational enclosure. Zebrafish. 7 (2), 149-154 (2010).
25. Akitake, C.M., Macurak, M., Halpern, M.E., & Goll, M.G. Transgenerational analysis of transcriptional silencing in zebrafish. Dev Biol. 352 (2), 191-201 (2011).
26. Boniface, E.J., Lu, J., Victoroff, T., Zhu, M., & Chen, W. FlEx-based transgenic reporter lines for visualization of Cre and Flp activity in live zebrafish. Genesis. 47 (7), 484-491 (2009).
27. Skarnes, W.C., et al. A public gene trap resource for mouse functional genomics. Nat Genet. 36 (6), 543-544 (2004).
28. Davidson, A.E., et al. Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposon. Dev Biol. 263 (2), 191-202 (2003).