Probing and sorting single cells-the application of a Raman-activated cell sorter

Spectroscopy Europe

Volumn 25, Issue 5, 2013, Pages 16-20

  Wang, Yun ^a,c   Song, Yizhi ^b   Zhu, Di ^a   Ji, Yuetong ^c   Wang, Tingting ^c   McIlvenna, David ^d   Yin, Huabing ^d   Xu, Jian ^c   Huang, Wei E ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b Wellsens Biotech Co Ltd   (China)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^d UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENT MOLECULES; FLUORESCENT SIGNALS; FUNCTIONAL UNITS; LIVING ORGANISMS; MICRO SPECTROSCOPY; SINGLE CELL STUDIES; STABLE-ISOTOPE PROBING;

CYTOLOGY; FLUORESCENCE; ISOTOPES; MOLECULES; RAMAN SPECTROSCOPY; SYNTHESIS (CHEMICAL);

CELLS;

EID: 84886498720     PISSN: 09660941     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. W. B. Whitman, D. C. Coleman and W. J. Wiebe, "Prokaryotes: The unseen majority", Proc Nat. Acad Sci USA 95, 6578 (1998).
2. P. C. Falkowski, T. Fencbel and E. F. Delong, "The microbial engines that drive earth's biogeochemical cycles", Science 320, 103-1 (2008). doi: 10.1125/science.1153213
3. W. E. Huang, R. I. Griffiths, L. P. Thompson, M. J. Bailey and A. S. Whiteley, "Raman microscopic analysis of single microbial cells", Anal. Chem. 76, 4452 (2004). doi: 10.1021/ac049753k (Pubitemid 39014020)
4. M. Q. Li, W. E. Huang, C. M. Gibson, P. W. Fowler and A. Jousset, "Stable isotope probing and Raman spectroscopy for monitoring carbon flow in a food chain and revealing metabolic pathway", Anal. Chem. 85, 1642 (2013). doi: 10.1021/ac302910x
5. M. Li, D. P. Canniffe, P. J. Jackson, S. FitzGerald, M. J. Dickman, P. A. Davison, J. G. Burgess, C. N. Hunter and W. E. Huang, "Rapid resonance Raman micrcspectroscopy to probe carbon dioxide fixation by single cells in microbial communities", ISMEJ. 6, 875 (2012). doi: 10.1038/ismej.2011.142
6. W. E. Huang, K. Stoecker, R. Griffiths, L. Newbold, H. Daims. A. S. Whiteley and M. Wagner, "Raman FISH: combining stable sotope Raman spectroscopy and fluorescence in situ hybridization for the single cell analysis of identity and function", Environ. Microbiol. 9, 1878 (2007). doi: 10.1111/J.1462-2920.2007.01352. X
7. W. E. Huang, A. Ferguson, A. C. Singer, K. Lawson, L. P. Ihompson, K. M. Kalin, M. J. Larkin, M. J. Bailey and A. S. Whiteley, "Resolving genetic functions within microbial populations: In situ analyses using rRNA and mRNA stable isotope probing coupled with single cell Raman-FISH", Appl. Environ. Microbiol. 75, 234 (2009). doi: 10.1128/AEM.01861-08
8. W. E. Huang, M. J. Bailey, L. P. Thompson, A. S. Whiteley and A. J. Spiers, "Single-cell Raman spectral profiles of Pseudornonas fluorescens SBV/25 reflects in vitro and in planta metabolic history", Microb. Ecol. 53, 414 (2007). doi: 10.1007/s00248-006-9138-5 (Pubitemid 46716627)
9. S. Haider, M. Wagner, M. C. Schmid, B. S. Sixt, J. G. Christian, G. Hacker, P. Pichler, K. Mechtler, A. Muller, C. Baranyi, E. R. Toenshoff, J. Montanaro and M. Horn", Raman microspectroscopy reveals longterm extracellular activity of chlornydiae", Mol. Microbiol. 77, 687 (2010). doi: 10.1111/J.1365-2958.2010.07241JC
10. J. S. Andrews, S. A. Kolfe, W. E. Huang, J. D. Scholes and S. A. Banwart, "Biofilm formation in environmental bacteria is influenced by different macromolecules depending on genus and species", Environ. Microbiol. 12. 2496 (2010). doi: 10.1111/J. K62-2920.2010.02223. X
11. W. E. Huang, A. D. Ward and A. S. Whiteley, "Raman tweezers sorting of single microbial cells", Environ. Microbiol. Rep. 1, 44 (2009). doi: 10.1111/j.1758-2229.2008.00002.x
12. W. E. Hjang, M. Q. Li, R. M. Jarvis, R. Goodacre and S. A. Banwart, "Shining light on the microbial world: the application of Raman microspectroscopy", Adv. Appl. Microbiol. 70, 153 (2010). doi: 10.1016/S0065-2164 (10) 70005-8
13. M. Q. Li, J. Xu, M. Romero-Gonzalez, S. A. Banwart and W. E. Huang, "Single cell Raman spectroscopy for cell sorting and imaging", Curr. Opin. Biotcchnol. 23, 56 (2012). doi: 10.1016/j.copbio.2011.11.019
14. M. Q. Li, P. C. Ashok, K. Dhoiakia and W.E. Huang, "Raman-activated cell counting for profiling carbon dioxide fixing microorganisms", J. Phys. Chem. A. 116, 5560 (2012). doi: 10.1021/jp212619n
15. H. Bolton (etal). A 1 0-Year Strategic Vision,. Forging the Future of the DOE JGI, rEd by US Department of Energy. (2012). www.jgidoegov/whoweare/10- Year-JGI-Strategic-Vision.pdf