Increased performance in genetic manipulation by modeling the dielectric properties of the rodent brain

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

Volumn , Issue , 2013, Pages 1615-1618

  Szczurkowska, Joanna ^a   Dal Maschio, Marco ^a   Cwetsch, Andrzej W ^a   Ghezzi, Diego ^a   Bony, Guillaume ^a   Alabastri, Alessandro ^a   Zaccaria, Remo Proietti ^a   Di Fabrizio, Enzo ^a   Ratto, Gian Michele ^b   Cancedda, Laura ^a  

^a ISTITUTO ITALIANO DI TECNOLOGIA   (Italy)

^b CNR   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BIPOLAR ELECTRODES; GENETIC MANIPULATIONS; OPTIMAL SOLUTIONS; STATE OF THE ART; STRONG ELECTRIC FIELDS; SURGICAL PROCEDURES; SURROUNDING ENVIRONMENT; THEORETICAL MODELING;

BRAIN; DISEASE CONTROL; DNA; ELECTRIC FIELDS; ELECTRODES; GENE EXPRESSION; MAMMALS;

DIELECTRIC PROPERTIES;

DNA;

ANIMAL; BRAIN; CHEMISTRY; COMPUTER SIMULATION; CONFOCAL MICROSCOPY; CYTOLOGY; ELECTRICITY; ELECTRODE; ELECTROPORATION; EMBRYOLOGY; IMAGE PROCESSING; MOUSE; PATHOLOGY; PROCEDURES; RAT; REPRODUCIBILITY; STEM CELL; THEORETICAL MODEL; THREE DIMENSIONAL IMAGING;

ANIMALS; BRAIN; COMPUTER SIMULATION; DNA; ELECTRICITY; ELECTRODES; ELECTROPORATION; IMAGE PROCESSING, COMPUTER-ASSISTED; IMAGING, THREE-DIMENSIONAL; MICE; MICROSCOPY, CONFOCAL; MODELS, THEORETICAL; RATS; REPRODUCIBILITY OF RESULTS; STEM CELLS;

EID: 84886461023     PISSN: 1557170X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/EMBC.2013.6609825     Document Type: Conference Paper

References (8)

1. LoTurco, J., Manent, J. B. and Sidiqi, F. "New and improved tools for in utero electroporation studies of developing cerebral cortex". Cereb. Cortex. 19 (Suppl 1), 120-125 (2009).
2. Swartz, M., Eberhart, J., Mastick, G. S. and Krull, C. E. "Sparking new frontiers: using in vivo electroporation for genetic manipulations". Dev. Biol. 233, 13-21(2001).
3. Matsuda, T. and Cepko, C. L. "Controlled expression of transgenes introduced by in vivo electroporation". Proc. Natl Acad. Sci. USA 104, 1027-1032 (2007).
4. Knopfel, T., "Genetically encoded optical indicators for the analy sis of neuronal circuits". Nat Rev Neurosci 13: (2012). 687-700.
5. Satkauskas, S., Ruzgys, P., Venslauskas, MS, "Towards the mechanisms for efficient gene transfer into cells and tissues by means of cell electro poration. Expert Opin Biol Ther. 2012 Mar;12(3):275-86.
6. Dal Maschio M., et al., "High-performance and Site-directed in Utero Electroporation by a Triple-electrode Probe". Nature Communications, 3:960. (2012).
7. Paxinos, G., Ashwell, K. W. S. and Törk, I. "Atlas of the Developing Rat Nervous System" 2nd edn (Academic Press, 1994).
8. Gabriel, S., Lau, R. W. and Gabriel, C. "The dielectric properties of biological tissues: III, parametric models for the dielectric spectrum of tissues". Phys. Med. Biol. 41, 2271-2293 (1996).