Pull-down analysis of interactions among jasmonic acid core signaling proteins

Methods in Molecular Biology

Volumn 1011, Issue , 2013, Pages 159-171

  Fonseca, Sandra ^a   Solano, Roberto ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

COI1; Coronatine; E. coli protein expression; Hormone dependent interaction; Jasmonate isoleucine; Jasmonic acid; JAZ; MBP fusion; Protein protein interaction; Pull down

Indexed keywords

CORE PROTEIN; EPITOPE; JASMONIC ACID; ARABIDOPSIS PROTEIN; CYCLOPENTANE DERIVATIVE; OXYLIPIN; PHYTOHORMONE; PLANT EXTRACT; SIGNAL PEPTIDE;

ARABIDOPSIS THALIANA; ARTICLE; ESCHERICHIA COLI; IMMUNOBLOTTING; NONHUMAN; PLANT TISSUE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INTERACTION; REPRESSOR GENE; ARABIDOPSIS; CHEMISTRY; METABOLISM; PROTEIN BINDING; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; BLOTTING, WESTERN; CYCLOPENTANES; ESCHERICHIA COLI; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; OXYLIPINS; PLANT EXTRACTS; PLANT GROWTH REGULATORS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; SIGNAL TRANSDUCTION;

EID: 84886438939     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-414-2_13     Document Type: Article

References (17)

1. Dharmasiri N, Dharmasiri S, Estelle M (2005) The F-box protein TIR1 is an auxin receptor. Nature 435:441-445
2. Fonseca S, Chini A, Hamberg M, Adie B, Porzel A, Kramell R, Miersch O, Wasternack C, Solano R (2009) (+)-7-iso -Jasmonoyl-L -isoleucine is the endogenous bioactive jasmonate. Nat Chem Biol 5:344-350
3. Katsir L, Schilmiller AL, Staswick PE, He SY, Howe GA (2008) COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine. Proc Natl Acad Sci USA 105:7100-7105
4. Chini A, Fonseca S, Fernández G, Adie B, Chico JM, Lorenzo O, García-Casado G, López-Vidriero I, Lozano FM, Ponce MR, Micol JL, Solano R (2007) The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448:666-671
5. Kepinski S, Leyser O (2005) The Arabidopsis F-box protein TIR1 is an auxin receptor. Nature 435:446-451
6. Pauwels L, Fernández Barbero G, Geerinck J, Tilleman S, Grunewald W, Cuéllar Pérez A, Chico JM, Vanden Bossche R, Sewell J, Gil E, García-Casado G, Witters E, Inzé D, Long JA, De Jaeger G, Solano R, Goossens A (2010) NINJA connects the co-repressor TOPLESS to jasmonate signalling. Nature 464:788-791
7. Feng S, Ma L, Wang X, Xie D, Dinesh-Kumar SP, Wei N, Deng XW (2003) The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses. Plant Cell 15: 1083-1094
8. Chini A, Fonseca S, Chico JM, Fernández-Calvo P, Solano R (2009) The ZIM domain mediates homo-and heteromeric interactions between Arabidopsis JAZ proteins. Plant J 59: 77-87
9. Fernández-Calvo P, Chini A, Fernández-Barbero G, Chico J-M, Gimenez-Ibanez S, Geerinck J, Eeckhout D, Schweizer F, Godoy M, Franco-Zorrilla JM, Pauwels L, Witters E, Puga MI, Paz-Ares J, Goossens A, Reymond P, De Jaeger G, Solano R (2011) The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of JA responses. Plant Cell 23:701-715
10. Clough SJ, Bent AF (1998) Floral dip: a simpli fied method for Agrobacterium -mediated transformation of Arabidopsis thaliana . Plant J 16:735-743
11. Niu Y, Figueroa P, Browse J (2011) Characterization of JAZ-interacting bHLH transcription factors that regulate jasmonate responses in Arabidopsis . J Exp Bot 62: 2143-2154
12. Melotto M, Mecey C, Niu Y, Chung HS, Katsir L, Yao J, Zeng W, Thines B, Staswick P, Browse J, Howe GA, He SY (2008) A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine-and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein. Plant J 55:979-988
13. Yan J, Zhang C, Gu M, Bai Z, Zhang W, Qi T, Cheng Z, Peng W, Luo H, Nan F, Wang Z, Xie D (2009) The Arabidopsis CORONATINE INSENSITIVE1 protein is a jasmonate receptor. Plant Cell 21:2220-2236
14. Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J (2007) JAZ repressor proteins are targets of the SCF COI1 complex during jasmonate signalling. Nature 448:661-665
15. Hammarström M, Hellgren N, Van Den Berg S, Berglund H, Härd T (2002) Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli . Protein Sci 11:313-321
16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473-497
17. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248-254