Transient expression assays in tobacco protoplasts

Methods in Molecular Biology

Volumn 1011, Issue , 2013, Pages 227-239

  Bossche, Robin Vanden ^a,b   Demedts, Brecht ^a,b   Vanderhaeghen, Rudy ^a,b   Goossens, Alain ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Automation; Promoters; Protoplasts; Tobacco BY 2; Transactivation; Transcription factors; Transient expression

Indexed keywords

LUCIFERASE; TRANSCRIPTION FACTOR; FIREFLY LUCIFERASE; VEGETABLE PROTEIN;

ARTICLE; ASSAY; AUTOMATION; CLONING; DATA ANALYSIS; GENE CONSTRUCT; GENE VECTOR; LUCIFERASE ASSAY; PRIORITY JOURNAL; PROMOTER REGION; PROTOPLAST; SUSPENSION CELL CULTURE; TOBACCO; TRANSACTIVATION; TRANSIENT EXPRESSION ASSAY; BIOSYNTHESIS; CULTURE TECHNIQUE; CYTOLOGY; GENE EXPRESSION; GENETIC TRANSFECTION; GENETICS; METABOLISM; PLASMID; REPORTER GENE;

CELL CULTURE TECHNIQUES; GENE EXPRESSION; GENES, REPORTER; LUCIFERASES, FIREFLY; PLANT PROTEINS; PLASMIDS; PROMOTER REGIONS, GENETIC; PROTOPLASTS; TOBACCO; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 84886389453     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-414-2_18     Document Type: Article

References (8)

1. Lee TI, Young RA (2000) Transcription of eukaryotic protein-coding genes. Annu Rev Genet 34:77-137
2. De Sutter V, Vanderhaeghen R, Tilleman S, Lammertyn F, Vanhoutte I, Karimi M, Inzé D, Goossens A, Hilson P (2005) Exploration of jasmonate signalling via automated and standardized transient expression assays in tobacco cells. Plant J 44:1065-1076
3. Wehner N, Hartmann L, Ehlert A, Böttner S, Oñate- Sánchez L, Dröge-Laser W (2011) Highthroughput protoplast transactivation (PTA) system for the analysis of Arabidopsis transcription factor function. Plant J 68:560-569
4. Tiwari SB, Wang X-J, Hagen G, Guilfoyle TJ (2001) AUX/IAA proteins are active repressors, and their stability and activity are modulated by auxin. Plant Cell 13:2809-2822
5. Bharti K, von Koskull-Döring P, Bharti S, Kumar P, Tintschl-Körbitzer A, Treuter E, Nover L (2004) Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant CREB binding protein ortholog HAC1. Plant Cell 16: 1521-1535
6. Pape S, Thurow C, Gatz C (2010) The Arabidopsis PR-1 promoter contains multiple integration sites for the coactivator NPR1 and the repressor SNI1. Plant Physiol 154: 1805-1818
7. De Boer K, Tilleman S, Pauwels L, Vanden Bossche R, De Sutter V, Vanderhaeghen R, Hilson P, Hamill JD, Goossens A (2011) APETALA2/ETHYLENE RESPONSE FACTOR and basic helix-loop-helix tobacco transcription factors cooperatively mediate jasmonate-elicited nicotine biosynthesis. Plant J 66:1053-1065
8. Karimi M, Inzé D, Depicker A (2002) GATEWAY™ vectors for Agrobacterium -mediated plant transformation. Trends Plant Sci 7:193-195