MRSA decolonization of cotton rat nares by a combination treatment comprising lysostaphin and the antimicrobial peptide ranalexin

Journal of Antimicrobial Chemotherapy

Volumn 68, Issue 11, 2013, Pages 2569-2575

  Desbois, Andrew P ^a,c   Sattar, Abdul ^b   Graham, Shirley ^a   Warn, Peter A ^b   Coote, Peter J ^a  

^a UNIVERSITY OF ST ANDREWS   (United Kingdom)

^b Euprotec Limited   (United Kingdom)

^c UNIVERSITY OF STIRLING   (United Kingdom)

Author keywords

Antibacterials; Combination; IleS; Infection control; Isoleucyl tRNA synthetase; Methicillin resistant Staphylococcus aureus; Mupirocin; Nasal carriage

Indexed keywords

HYDROXYPROPYLMETHYLCELLULOSE; LYSOSTAPHIN; PSEUDOMONIC ACID; RANALEXIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBIOTIC RESISTANCE; ANTIMICROBIAL THERAPY; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATION; CONTROLLED STUDY; DRUG EFFICACY; HUMAN; IN VIVO STUDY; INOCULATION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; RAT; SHORT COURSE THERAPY;

ANTIBACTERIALS; COMBINATION; ILES; INFECTION CONTROL; ISOLEUCYL-TRNA SYNTHETASE; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MUPIROCIN; NASAL CARRIAGE;

ADMINISTRATION, TOPICAL; ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIAL LOAD; CARRIER STATE; DRUG THERAPY, COMBINATION; GELS; LYSOSTAPHIN; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MODELS, ANIMAL; NOSE; PEPTIDES, CYCLIC; SIGMODONTINAE; STAPHYLOCOCCAL INFECTIONS; TREATMENT OUTCOME;

EID: 84886257742     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkt243     Document Type: Article

References (24)

1. Kallen AJ, Wilson CT, Larson RJ. Perioperative intranasal mupirocin for the prevention of surgical-site infections: systematic review of the literature and meta-analysis. Infect Control Hosp Epidemiol 2005; 26: 916-22.
2. Pofahl W, Goettler C, Ramsey K et al. Active surveillance screening of MRSA and eradication of the carrier state decreases surgical-site infections caused by MRSA. J Am Coll Surg 2009; 208: 981-6.
3. Bode LG, Kluytmans JA, Wertheim HF et al. Preventing surgical-site infections in nasal carriers of Staphylococcus aureus. N Engl J Med 2010; 362: 9-17.
4. Simor AE, Stuart TL, Louie L et al. Mupirocin-resistant, methicillin-resistant Staphylococcus aureus strains in Canadian hospitals. Antimicrob Agents Chemother 2007; 51: 3880-6.
5. Coates T, Bax R, Coates A. Nasal decolonization of Staphylococcus aureus with mupirocin: strengths, weaknesses and future prospects. J Antimicrob Chemother 2009; 64: 9-15.
6. Gilpin D, Small S, Bakkshi S et al. Efficacy of a standard meticillin-resistant Staphylococcus aureus decolonisation protocol in routine clinical practice. J Hosp Infect 2010; 75: 92-8.
7. Liu C, Bayer A, Cosgrove SE et al. Clinical practice guidelines by the Infectious Diseases Society of America for the treatment of methicillin-resistant Staphylococcus aureus infections in adults and children. Clin Infect Dis 2011; 52: 285-92.
8. Hurdle J, O'Neill A, Ingham E et al. Analysis of mupirocin resistance and fitness in Staphylococcus aureus by molecular genetic and structural modeling techniques. Antimicrob Agents Chemother 2004; 48: 4366-76.
9. Cadilla A, David MZ, Daum RS et al. Association of high-level mupirocin resistance and multidrug-resistant methicillin-resistant Staphylococcus aureus at an academic center in the midwestern United States. J Clin Microbiol 2011; 49: 95-100.
10. Lee AS, Macedo-Vinas M, Francois P et al. Impact of combined low-level mupirocin and genotypic chlorhexidine resistance on persistent methicillin-resistant Staphylococcus aureus carriage after decolonization therapy: a case-control study. Clin Infect Dis 2011; 52: 1422-30.
11. Seah C, Alexander DC, Louie L et al. MupB, a new high-level mupirocin resistance mechanism in Staphylococcus aureus. Antimicrob Agents Chemother 2012; 56: 1916-20.
12. Grohs P, Kitzis MD, Gutmann L. In vitro bactericidal activities of linezolid in combination with vancomycin, gentamicin, ciprofloxacin, fusidic acid, and rifampin against Staphylococcus aureus. Antimicrob Agents Chemother 2003; 47: 418-20.
13. Hammer KA, Carson CF, Riley TV. Effects of Melaleuca alternifolia (tea tree) essential oil and the major monoterpene component terpinen-4-ol on the development of single- and multistep antibiotic resistance and antimicrobial susceptibility. Antimicrob Agents Chemother 2012; 56: 909-15.
14. Graham S, Coote PJ. Potent, synergistic inhibition of Staphylococcus aureus upon exposure to a combination of the endopeptidase lysostaphin and the cationic peptide ranalexin. J Antimicrob Chemother 2007; 59: 759-62.
15. Desbois AP, Gemmell CG, Coote PJ. In vivo efficacy of the antimicrobial peptide ranalexin in combination with the endopeptidase lysostaphin against wound and systemic meticillin-resistant Staphylococcus aureus (MRSA) infections. Int J Antimicrob Agents 2010; 35: 559-65.
16. Desbois AP, Lang S, Gemmell CG et al. Surface disinfection properties of the combination of an antimicrobial peptide, ranalexin, with an endopeptidase, lysostaphin, against methicillin-resistant Staphylococcus aureus (MRSA). J Appl Microbiol 2010; 108: 723-30.
17. Kokai-Kun JF, Walsh SM, Chanturiya T et al. Lysostaphin cream eradicates Staphylococcus aureus nasal colonization in a cotton rat model. Antimicrob Agents Chemother 2003; 47: 1589-97.
18. Kokai-Kun JF. The cotton rat as a model for Staphylococcus aureus nasal colonization in humans: cotton rat S. aureus nasal colonization model. Methods Mol Biol 2008; 431: 241-54.
19. Freitas E, Harris R, Blake R et al. Prevalence of USA300 strain type of methicillin-resistant Staphylococcus aureus among patients with nasal colonisation identified with active surveillance. Infect Control Hosp Epidemiol 2010; 31: 469-75.
20. Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically-Ninth Edition: Approved Standard M07-A9. CLSI,Wayne, PA, USA, 2012.
21. Bannerman TL, Hancock GA, Tenover FC et al. Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J Clin Microbiol 1995; 33: 551-5.
22. Leonard RB,Mayer J, SasserM et al. Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak. J Clin Microbiol 1995; 33: 2723-7.
23. van Rijen M, Bonten M,Wenzel R et al. Mupirocin ointment for preventing Staphylococcus aureus infections in nasal carriers. Cochrane Database Syst Rev 2008; issue 4: CD006216.
24. Desbois AP, Coote PJ. Bactericidal synergy of lysostaphin in combination with antimicrobial peptides. Eur J Clin Microbiol Infect Dis 2011; 30: 1015-21.