Determination of hypoglycaemia induced by insulin or its synthetic analogues post mortem

Drug Testing and Analysis

Volumn 5, Issue 9-10, 2013, Pages 802-807

  Hess, C ^a   Madea, B ^a   Daldrup, T ^b   Musshoff, F ^a  

^a UNIVERSITY HOSPITAL BONN   (Germany)

^b UNIVERSITY HOSPITAL DÜSSELDORF   (Germany)

Author keywords

Hypoglycaemia; Insulin; LC MS; Post mortem

Indexed keywords

BLOOD; DRUG PRODUCTS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDES;

HUMAN INSULINS; HYPOGLYCAEMIA; IMMUNOPURIFICATION; LC-MS; LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY; MAGNETIC BEADS; MAGNETIC LIQUIDS; POST MORTEM; SYNTHETIC ANALOGUES; VITREOUS HUMOR;

INSULIN;

C PEPTIDE; CITALOPRAM; FENTANYL; GLITAZONE DERIVATIVE; GLUCOSE; HUMAN INSULIN; INSULIN LISPRO; LACTIC ACID; METHANOL; METOCLOPRAMIDE; MORPHINE; PROINSULIN; RECOMBINANT HUMAN INSULIN; SULFONYLUREA; SYNTHETIC INSULIN;

ADULT; ARTICLE; AUTOPSY; BLOOD SAMPLING; CASE REPORT; DRUG BLOOD LEVEL; DRUG BRAIN LEVEL; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GLUCOSE BLOOD LEVEL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; HYPOGLYCEMIA; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY; URINALYSIS; VITREOUS BODY;

HYPOGLYCAEMIA; INSULIN; LC-MS; POST MORTEM;

AUTOPSY; CHROMATOGRAPHY, LIQUID; FATAL OUTCOME; FEMALE; FORENSIC TOXICOLOGY; HUMANS; HYPOGLYCEMIA; HYPOGLYCEMIC AGENTS; INSULIN; MIDDLE AGED;

EID: 84886004256     PISSN: 19427603     EISSN: 19427611     Source Type: Journal    
DOI: 10.1002/dta.1500     Document Type: Article

References (42)

1. K.M. MacLeod, D.A. Hepburn, B.M. Frier. Frequency and morbidity of severe hypoglycaemia in insulin-treated diabetic patients. Diabet. Med. 1993, 10, 238.
2. M.W. Lai, W. Klein-Schwartz, G.C. Rodgers, J.Y. Abrams, D.A. Haber, A.C. Bronstein, K.M. Wruk. 2005 annual report of the American association of poison control centers' national poisoning and exposure database. Clin. Toxicol. 2006, 44, 803.
3. A.C. Bronstein, D.A. Spyker, L.R. Cantilena Jr, J.L. Green, B.H. Rumack, S.E. Heard. 2007 annual report of the American association of poison control centers' national poison data system (NPDS): 25th annual report. Clin. Toxicol. 2008, 46, 927.
4. I.B. Hirsch. Insulin analogues. New Engl. J. Med. 2005, 352, 174.
5. P.C. Rodriguez, E.A. Lizondo, M.J. Lopez Garcia, C.L. Escriva, L.R. Alpera, P.C. Collado. A study of variability in glycaemia in children and adolescents with diabetes mellitus type 1 on treatment with insulin glargine. An. Pediatr. 2008, 69, 31.
6. B. Megarbane, N. Deye, V. Bloch, R. Sonneville, C. Collet, J.M. Launay, F.J. Baud. Intentional overdose with insulin: Prognostic factors and toxicokinetic/toxicodynamic profiles. Crit. Care 2007, 11, R115.
7. M.A. von Mach, M. Gauer, S. Meyer, B. Omogbehin, H. Schinzel, P.H. Kann, L.S. Weilemann. Antidiabetic medications in overdose: A comparison of the inquiries made to a regional poisons unit regarding original sulfonylureas, biguanides and insulin. Int. J. Clin. Pharmacol. Ther. 2006, 44, 51.
8. R. Arem, W. Zoghbi. Insulin overdose in eight patients: insulin pharmacokinetics and review of the literature. Medicine 1985, 64, 323.
9. V. Marks. Murder by insulin. Med. Leg. J. 1999, 67, 147.
10. V. Marks. Insulin murders. Med. Leg. J. 2009, 77, 39.
11. V. Marks. Murder by insulin: Suspected, purported and proven-a review. Drug Test. Anal. 2009, 1, 162.
12. T.A. Mork, C.T. Killeen, N.K. Patel, J.M. Dohnal, H.C. Karydes, J.B. Leikin. Massive insulin overdose managed by monitoring daily insulin levels. Am. J. Ther. 2011, 18, e162.
13. M. Brvar, M. Mozina, M. Bunc. Prolonged hypoglycaemia after insulin lispro overdose. Eur. J. Emerg. Med. 2005, 12, 234.
14. R.P. Green, A.S. Hollander, M. Thevis, A. Thomas, D.J. Dietzen. Detection of surreptitious administration of analog insulin to an 8-week-old infant. Pediatrics 2010, 125, e1236.
15. M. Thevis, A. Thomas, W. Schanzer, P. Ostman, I. Ojanpera. Measuring insulin in human vitreous humour using LC-MS/MS. Drug Test. Anal. 2012, 4, 53.
16. C. Hess, A. Thomas, M. Thevis, B. Stratmann, W. Quester, D. Tschoepe, B. Madea, F. Musshoff. Simultaneous determination and validated quantification of human insulin and its synthetic analogues in human blood serum by immunoaffinity purification and liquid chromatography-mass spectrometry. Anal. Bioanal. Chem. 2012, 404, 1813.
17. M. Matsumura, A. Nakashima, Y. Tofuku. Electrolyte disorders following massive insulin overdose in a patient with type 2 diabetes. Intern. Med. 2000, 39, 55.
18. D. Uchida, S. Ohigashi, S. Hikita, N. Kitamura, M. Motoyoshi, I. Tatsuno. Acute pulmonary edema caused by hypoglycemia due to insulin overdose. Intern. Med. 2004, 43, 1056.
19. P. Jolliet, X. Leverve, C. Pichard. Acute hepatic steatosis glucose determinations complicating massive insulin overdose and excessive glucose administration. Intens. Care Med. 2001, 27, 313.
20. I. Hood, H. Mirchandani, J. Monforte, W. Stacer. Immunohistochemical demonstration of homicidal insulin injection site. Arch. Pathol. Lab. Med. 1986, 110, 973.
21. H. Gormsen, A. Lund. The diagnostic value of postmortem blood in cases of diabetes mellitus. Forensic Sci. Int. 1985, 28, 103.
22. W.Q. Sturner, G.E. Ganther Jr. Postmortem vitreous glucose determinations. J. Forensic Sci. 1964, 9, 485.
23. C. Peclet, P. Picotte, F. Jobin. The use of vitreous humor levels of glucose, lactic acid and blood levels of acetone to establish antemortem hyperglycemia in diabetics. Forensic Sci. Int. 1994, 65, 1.
24. B. Zilg, K. Alkass, S. Berg, H. Druid. Postmortem identification of hyperglycemia. Forensic Sci. Int. 2009, 185, 89.
25. M.Z. Karlovsek. Diagnostic values of combined glucose and lactate values in cerebrospinal fluid and vitreous humour--our experiences. Forensic Sci. Int. 2004, 146, S19.
26. S. Ritz, H.J. Kaatsch. Postmortem diagnosis of fatal diabetic metabolic dyscontrol: What is the significance of cerebrospinal fluid and vitreous body total values and HbA1? Pathologe 1990, 11, 158.
27. J.I. Coe. Postmortem chemistries on human vitreous humor. Am. J. Clin. Pathol. 1969, 51, 741.
28. E.A. De Letter, M.H. Piette. Can routinely combined analysis of glucose and lactate in vitreous humour be useful in current forensic practice? Am. J. Forensic Med. Pathol. 1998, 19, 335.
29. V. Marks, J.D. Teale. Hypoglycaemia in the adult. Baillieres Clin. Endocrinol. Metab. 1993, 7, 705.
30. D.R. Cabaleiro, D. Stockl, J.M. Kaufman, T. Fiers, L.M. Thienpont. Feasibility of standardization of serum C-peptide immunoassays with isotope-dilution liquid chromatography-tandem mass spectrometry. Clin. Chem. 2006, 52, 1193.
31. C. Wunder. Systematische Untersuchungen zur Problematik postmortaler Insulin-Instabilität, thesis. Institute of Forensic Medicine, University Frankfurt, Germany, 2011.
32. M. Muhm, A. Berzlanovich. Zur Moglichkeit der post-mortalen Diagnose einer exogenen Insulinintoxikation, Zbl. Rechtsmed 1991, 36, 175.
33. S. O'Rahilly, M.A. Burnett, R.F. Smith, J.H. Darley, R.C. Turner. Haemolysis affects insulin but not C-peptide immunoassay. Diabetologia 1987, 30, 394.
34. I.W. Campbell, J.G. Ratcliffe. Suicidal insulin overdose managed by excision of insulin injection site. Brit. Med. J. (Clin. Res. Ed.) 1982, 285, 408.
35. A. Thomas, W. Schanzer, P. Delahaut, M. Thevis. Sensitive and fast identification of urinary human, synthetic and animal insulin by means of nano-UPLC coupled with high-resolution/high-accuracy mass spectrometry. Drug Test. Anal. 2009, 1, 219.
36. D.C. Winston. Suicide via insulin overdose in nondiabetics: The New Mexico experience. Am. J. Forensic Med. Pathol. 2000, 21, 237.
37. W.Q. Sturner, R.S. Putnam. Suicidal insulin poisoning with nine day survival: Recovery in bile at autopsy by radioimmunoassay. J. Forensic Sci. 1972, 17, 514.
38. M. Thevis, A. Thomas, W. Schanzer. Mass spectrometric determination of insulins and their degradation products in sports drug testing. Mass Spectrom. Rev. 2008, 27, 35.
39. J.I. Coe. Postmortem chemistry update. Emphasis on forensic application. Am. J. Forensic Med. Pathol. 1993, 14, 91.
40. J. Nowicka, R. Skowronek, E. Czech, J. Kulikowska, Z. Olszowy. Comments on 'Measuring insulin in human vitreous humour using LC-MS/MS' by Thevis et al. Drug Test. Anal. 2013; 5(2): 133-4.
41. M. Thevis, A. Thomas, P. Delahaut, A. Bosseloir, W. Schanzer. Qualitative determination of synthetic analogues of insulin in human plasma by immunoaffinity purification and liquid chromatography-tandem mass spectrometry for doping control purposes. Anal. Chem. 2005, 77, 3579.
42. M. Thevis, A. Thomas, P. Delahaut, A. Bosseloir, W. Schanzer. Doping control analysis of intact rapid-acting insulin analogues in human urine by liquid chromatography-tandem mass spectrometry. Anal. Chem. 2006, 78, 1897.