CDPK1 from Ginger Promotes Salinity and Drought Stress Tolerance without Yield Penalty by Improving Growth and Photosynthesis in Nicotiana tabacum

PLoS ONE

Volumn 8, Issue 10, 2013, Pages

  Vivek, Padmanabhan Jayanthi ^a   Tuteja, Narendra ^b   Soniya, Eppurathu Vasudevan ^a  

^a RAJIV GANDHI CENTRE FOR BIOTECHNOLOGY   (India)

^b INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE I; CALMODULIN; COMPLEMENTARY DNA; CALCIUM-DEPENDENT PROTEIN KINASE; PRIMER DNA; PROTEIN KINASE;

ARTICLE; CHLOROPHYLL CONTENT; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DROUGHT STRESS; EPIDERMIS CELL; ERD1 GENE; GENE AMPLIFICATION; GENE FUNCTION; GENE OVEREXPRESSION; GERMINATION; GINGER; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PHOTOSYNTHESIS; PLANT GENE; PLANT GROWTH; PLANT WATER CONTENT; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; PROTEIN EXPRESSION; RD21A GENE; RHIZOME; SALT STRESS; SIGNAL TRANSDUCTION; TOBACCO; UPREGULATION; ZOCDPK1 GENE; AMINO ACID SEQUENCE; CHEMISTRY; CLUSTER ANALYSIS; CONFOCAL MICROSCOPY; DNA SEQUENCE; DROUGHT; GENE EXPRESSION REGULATION; GENE TRANSFER; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; PHYLOGENY; PHYSIOLOGICAL STRESS; PHYSIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALINITY; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLUSTER ANALYSIS; DNA PRIMERS; DROUGHTS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE TRANSFER TECHNIQUES; GINGER; MICROSCOPY, CONFOCAL; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PHYLOGENY; PLANTS, GENETICALLY MODIFIED; PROTEIN KINASES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SALINITY; SEQUENCE ANALYSIS, DNA; STRESS, PHYSIOLOGICAL; TOBACCO;

EID: 84885981466     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0076392     Document Type: Article

References (82)

1. Mahajan S, Tuteja N, (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444: 139-158. doi:10.1016/j.abb.2005.10.018. PubMed: 16309626.
2. Mahajan S, Pandey GK, Tuteja N, (2008) Calcium- and salt-stress signaling in plants: shedding light on SOS pathway. Arch Biochem Biophys 471: 146-158. doi:10.1016/j.abb.2008.01.010. PubMed: 18241665.
3. Kudla J, Batistic O, Hashimoto K, (2010) Calcium signals: the lead currency of plant information processing. Plant Cell 22: 541-563. doi:10.1105/tpc.109.072686. PubMed: 20354197.
4. Harmon AC, Gribskov M, Harper JF, (2000) CDPKs- a kinase for every Ca2+ signal? Trends Plant Sci 5: 154-159. doi:10.1016/S1360-1385(00)01577-6. PubMed: 10740296.
5. Tuteja N, Mahajan S, (2007) Calcium signaling network in plants: an overview. Plant Signal Behav 2: 79-85. doi:10.4161/psb.2.2.4176. PubMed: 19516972.
6. Harmon AC, (2003) Calcium-regulated protein kinases of plants. Gravit Space Biol Bull 16: 83-90. PubMed: 12959135.
7. Harper JF, Harmon AC, (2005) Plants, symbiosis and parasites: a calcium signalling connection. Nat Rev Mol Cell Biol 6: 555-566. doi:10.1038/nrm1679. PubMed: 16072038.
8. Christodoulou J, Malmendal A, Harper JF, Chazin WJ, (2004) Evidence for differing roles for each lobe of the calmodulin-like domain in a calcium-dependent protein kinase. J Biol Chem 279: 29092-29100. doi:10.1074/jbc.M401297200. PubMed: 15126505.
9. Asano T, Tanaka N, Yang G, Hayashi N, Komatsu S, (2005) Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice. Plant Cell Physiol 46: 356-366. doi:10.1093/pcp/pci035. PubMed: 15695435.
10. Cheng SH, Willmann MR, Chen HC, Sheen J, (2002) Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129: 469-485. doi:10.1104/pp.005645. PubMed: 12068094.
11. Hrabak EM, Chan CW, Gribskov M, Harper JF, Choi JH, et al. (2003) The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132: 666-680. doi:10.1104/pp.102.011999. PubMed: 12805596.
12. Ray S, Agarwal P, Arora R, Kapoor S, Tyagi AK, (2007) Expression analysis of calcium-dependent protein kinase gene family during reproductive development and abiotic stress conditions in rice (Oryza sativa L. ssp. indica). Mol Genet Genomics 278: 493-505.
13. Harper JF, Breton G, Harmon A, (2004) Decoding Ca(2+) signals through plant protein kinases. Annu Rev Plant Biol 55: 263-288. doi:10.1146/annurev.arplant.55.031903.141627. PubMed: 15377221.
14. Dammann C, Ichida A, Hong B, Romanowsky SM, Hrabak EM, et al. (2003) Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis. Plant Physiol 132: 1840-1848. doi:10.1104/pp.103.020008. PubMed: 12913141.
15. Lu SX, Hrabak EM, (2002) An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum. Plant Physiol 128: 1008-1021. doi:10.1104/pp.010770. PubMed: 11891256.
16. Asano T, Hayashi N, Kikuchi S, Ohsugi R, (2012) CDPK-mediated abiotic stress signaling. Plant Signal Behav 7: 817-821. doi:10.4161/psb.20351. PubMed: 22751324.
17. Hubbard KE, Siegel RS, Valerio G, Brandt B, Schroeder JI, (2012) Abscisic acid and CO2 signalling via calcium sensitivity priming in guard cells, new CDPK mutant phenotypes and a method for improved resolution of stomatal stimulus-response analyses. Ann Bot 109: 5-17. doi:10.1093/aob/mcr252. PubMed: 21994053.
18. Jaworski K, Pawełek A, Kopcewicz J, Szmidt-Jaworska A, (2012) The calcium-dependent protein kinase (PnCDPK1) is involved in Pharbitis nil flowering. J Plant Physiol 169: 1578-1585. doi:10.1016/j.jplph.2012.05.025. PubMed: 22840323.
19. Ma SY, Wu WH, (2007) AtCPK23 functions in Arabidopsis responses to drought and salt stresses. Plant Mol Biol 65: 511-518. doi:10.1007/s11103-007-9187-2. PubMed: 17541706.
20. Wan B, Lin Y, Mou T, (2007) Expression of rice Ca(2+)-dependent protein kinases (CDPKs) genes under different environmental stresses. FEBS Lett 581: 1179-1189. doi:10.1016/j.febslet.2007.02.030. PubMed: 17336300.
21. Yoon GM, Cho HS, Ha HJ, Liu JR, Lee HS, (1999) Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein. Plant Mol Biol 39: 991-1001. doi:10.1023/A:1006170512542. PubMed: 10344204.
22. Urao T, Katagiri T, Mizoguchi T, Yamaguchi-Shinozaki K, Hayashida N, et al. (1994) Two genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana. Mol Gen Genet 244: 331-340. PubMed: 8078458.
23. Xu J, Tian YS, Peng RH, Xiong AS, Zhu B, et al. (2010) AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis. Planta 231: 1251-1260. doi:10.1007/s00425-010-1122-0. PubMed: 20217124.
24. Mehlmer N, Wurzinger B, Stael S, Hofmann-Rodrigues D, Csaszar E, et al. (2010) The Ca(2+)-dependent protein kinase CPK3 is required for MAPK-independent salt-stress acclimation in Arabidopsis. Plant J. PubMed: 20497378.
25. Komatsu S, Yang G, Khan M, Onodera H, Toki S, et al. (2007) Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants. Mol Genet Genomics 277: 713-723. doi:10.1007/s00438-007-0220-6. PubMed: 17318583.
26. Saijo Y, Hata S, Kyozuka J, Shimamoto K, Izui K, (2000) Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants. Plant J 23: 319-327. doi:10.1046/j.1365-313x.2000.00787.x. PubMed: 10929125.
27. Ulloa RM, Raíces M, MacIntosh GC, Maldonado S, Téllez-Iñón MT, (2002) Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in Solanum tuberosum. Physiol Plant 115: 417-427. doi:10.1034/j.1399-3054.2002.1150312.x. PubMed: 12081535.
28. Yu XC, Li MJ, Gao GF, Feng HZ, Geng XQ, et al. (2006) Abscisic acid stimulates a calcium-dependent protein kinase in grape berry. Plant Physiol 140: 558-579. doi:10.1104/pp.105.074971. PubMed: 16407437.
29. Zhu SY, Yu XC, Wang XJ, Zhao R, Li Y, et al. (2007) Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis. Plant Cell 19: 3019-3036. doi:10.1105/tpc.107.050666. PubMed: 17921317.
30. Szczegielniak J, Borkiewicz L, Szurmak B, Lewandowska-Gnatowska E, Statkiewicz M, et al. (2012) Maize calcium-dependent protein kinase (ZmCPK11): local and systemic response to wounding, regulation by touch and components of jasmonate signaling. Physiol Plant 146: 1-14. doi:10.1111/j.1399-3054.2012.01587.x. PubMed: 22289134.
31. Wurzinger B, Mair A, Pfister B, Teige M, (2011) Cross-talk of calcium-dependent protein kinase and MAP kinase signaling. Plant Signal Behav 6: 8-12. doi:10.4161/psb.6.1.14012. PubMed: 21248475.
32. Thompson JD, Higgins DG, Gibson TJ, (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680. doi:10.1093/nar/22.22.4673. PubMed: 7984417.
33. Nicot N, Hausman JF, Hoffmann L, Evers D, (2005) Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 56: 2907-2914. doi:10.1093/jxb/eri285. PubMed: 16188960.
34. Curtis MD, Grossniklaus U, (2003) A gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol 133: 462-469. doi:10.1104/pp.103.027979. PubMed: 14555774.
35. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, et al. (1985) A simple and general method for transferring genes into plants. Science 227: 1229-1231. doi:10.1126/science.227.4691.1229. PubMed: 17757866.
36. Méchin V, Damerval C, Zivy M, (2007) Total protein extraction with TCA-acetone. In: Plant proteomics: methods and protocols, vol 355, Methods in molecular biology. Clifton: Humana Press. pp pp. 1-8.
37. Goel D, Singh AK, Yadav V, Babbar SB, Bansal KC, (2010) Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.). Protoplasma 245: 133-141.
38. Lichtenthaler HK, (1987) Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. Methods Enzymol 148: 350-366. doi:10.1016/0076-6879(87)48036-1.
39. Tuteja N, (2010) A method to confer salinity stress tolerance to plants by helicase overexpression. Methods Mol Biol 587: 377-387. PubMed: 20225163.
40. Schreiber U, Klughammer C, Neubauer C, (1988) Measuring P700 absorbance changes around 830 nm with a new type of pulse modulation system. Z Naturforsch 43: 686-669.
41. Wydro M, Kozubek E, Lehmann P, (2006) Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana. Acta Biochim Pol 53: 289-298. PubMed: 16582986.
42. Hanks SK, Quinn AM, Hunter T, (1988) The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241: 42-52. doi:10.1126/science.3291115. PubMed: 3291115.
43. Dingwall C, Laskey RA, (1991) Nuclear targeting sequences--a consensus? Trends Biochem Sci 16: 478-481. doi:10.1016/0968-0004(91)90184-W. PubMed: 1664152.
44. Wasternack C, (2007) Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann Bot 100: 681-697. doi:10.1093/aob/mcm079. PubMed: 17513307.
45. Schenk PM, Kazan K, Wilson I, Anderson JP, Richmond T, et al. (2000) Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc Natl Acad Sci U S A 97: 11655-11660. doi:10.1073/pnas.97.21.11655. PubMed: 11027363.
46. Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K, (1993) Characterization of cDNA for a dehydration-inducible gene that encodes a CLP A, B-like protein in Arabidopsis thaliana L. Biochem Biophys Res Commun 196: 1214-1220. doi:10.1006/bbrc.1993.2381. PubMed: 7504470.
47. Koizumi M, Yamaguchi-Shinozaki K, Tsuji H, Shinozaki K, (1993) Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana. Gene 129: 175-182. doi:10.1016/0378-1119(93)90266-6. PubMed: 8325504.
48. Gilmour SJ, Zarka DG, Stockinger EJ, Salazar MP, Houghton JM, et al. (1998) Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J 16: 433-442. doi:10.1046/j.1365-313x.1998.00310.x. PubMed: 9881163.
49. Narusaka Y, Nakashima K, Shinwari ZK, Sakuma Y, Furihata T, Abe H, Narusaka M, Shinozaki K, Yamaguchi-Shinozaki K, (2003) Interaction between two cis-acting elements, ABRE and DRE, in ABA-independent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses. Plant J 34: 137-148. doi:10.1046/j.1365-313X.2003.01708.x. PubMed: 12694590.
50. Kasuga M, Miura S, Shinozaki K, Yamaguchi-Shinozaki K, (2004) A Combination of the Arabidopsis DREB1A Gene and Stress-Inducible rd29A Promoter Improved Drought- and Low-Temperature Stress Tolerance in Tobacco by Gene Transfer. Plant Cell Physiol 45: 346-350. doi:10.1093/pcp/pch037. PubMed: 15047884.
51. Sheen J, (1996) Ca2+-dependent protein kinases and stress signal transduction in plants. Science 274: 1900-1902. doi:10.1126/science.274.5294.1900. PubMed: 8943201.
52. Zou JJ, Wei FJ, Wang C, Wu JJ, Ratnasekera D, et al. (2010) Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid- and Ca2+-mediated stomatal regulation in response to drought stress. Plant Physiol 154: 1232-1243. doi:10.1104/pp.110.157545. PubMed: 20805328.
53. Chung E, Oh SK, Park JM, Choi D, (2007) Expression and promoter analyses of pepper CaCDPK4 (Capsicum annuum calcium dependent protein kinase 4) during plant defense response to incompatible pathogen. Plant Pathol 23: 76-89. doi:10.5423/PPJ.2007.23.2.076.
54. Raichaudhuri A, Bhattacharyya R, Chaudhuri S, Chakrabarti P, Dasgupta M, (2006) Domain analysis of a groundnut calcium-dependent protein kinase: nuclear localization sequence in the junction domain is coupled with nonconsensus calcium binding domains. J Biol Chem 281: 10399-10409. doi:10.1074/jbc.M511001200. PubMed: 16464867.
55. Vitart V, Christodoulou J, Huang JF, Chazin WJ, Harper JF, (2000) Intramolecular activation of a Ca(2+)-dependent protein kinase is disrupted by insertions in the tether that connects the calmodulin-like domain to the kinase. Biochemistry 39: 4004-4011. doi:10.1021/bi992373m. PubMed: 10747788.
56. Liu G, Chen J, Wang X, (2006) VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Plant Cell Environ 29: 2091-2099. doi:10.1111/j.1365-3040.2006.01582.x. PubMed: 17081243.
57. Syam Prakash SR, Jayabaskaran C, (2006) Expression and localization of calcium-dependent protein kinase isoforms in chickpea. J Plant Physiol 163: 1135-1149. doi:10.1016/j.jplph.2006.04.002. PubMed: 16716453.
58. Gang DR, Ma X, (2008) Ginger and Turmeric, Ancient Spices and Modern Medicines. In: Moore PH, Ming R, eds. Genomics of Tropical Crop Plants. Springer Verlag.
59. Raíces M, Gargantini PR, Chinchilla D, Crespi M, Téllez-Iñón MT, et al. (2003) Regulation of CDPK isoforms during tuber development. Plant Mol Biol 52: 1011-1024. doi:10.1023/A:1025478315648. PubMed: 14558661.
60. Muñiz García MN, Giammaria V, Grandellis C, Téllez-Iñón MT, Ulloa RM, et al. (2012) Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro. Planta 235: 761-778. doi:10.1007/s00425-011-1540-7. PubMed: 22042328.
61. Asano T, Hayashi N, Kobayashi M, Aoki N, Miyao A, et al. (2012) A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance. Plant J 69: 26-36. doi:10.1111/j.1365-313X.2011.04766.x. PubMed: 21883553.
62. Abbasi F, Onodera H, Toki S, Tanaka H, Komatsu S, (2004) OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath. Plant Mol Biol 55: 541-552. doi:10.1007/s11103-004-1178-y. PubMed: 15604699.
63. Witte CP, Keinath N, Dubiella U, Demoulière R, Seal A, et al. (2010) Tobacco calcium-dependent protein kinases are differentially phosphorylated in vivo as part of a kinase cascade that regulates stress response. J Biol Chem 285: 9740-9748. doi:10.1074/jbc.M109.052126. PubMed: 20118232.
64. Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K, (1994) Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) in Arabidopsis thaliana L.: identification of three ERDs as HSP cognate genes. Plant Mol Biol 25: 791-798. doi:10.1007/BF00028874. PubMed: 8075396.
65. Thomashow MF, (1999) PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms. Annu Rev Plant Physiol Plant Mol Biol 50: 571-599. doi:10.1146/annurev.arplant.50.1.571. PubMed: 15012220.
66. Zhu JK, (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53: 247-273. doi:10.1146/annurev.arplant.53.091401.143329. PubMed: 12221975.
67. Shinozaki K, Yamaguchi-Shinozaki K, Seki M, (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6: 410-417. doi:10.1016/S1369-5266(03)00092-X. PubMed: 12972040.
68. Sanan-Mishra N, Pham XH, Sopory SK, Tuteja N, (2005) Pea DNA helicase 45 overexpression in tobacco confers high salinity tolerance without affecting yield. Proc Natl Acad Sci U S A 102: 509-514. doi:10.1073/pnas.0406485102. PubMed: 15630095.
69. Dang HQ, Tran NQ, Gill SS, Tuteja R, Tuteja N, (2011) A single subunit MCM6 from pea promotes salinity stress tolerance without affecting yield. Plant Mol Biol 76: 19-34. doi:10.1007/s11103-011-9758-0. PubMed: 21365356.
70. Zhao R, Sun HL, Mei C, Wang XJ, Yan L, et al. (2011) The Arabidopsis Ca(2+)-dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth. New Phytol 192: 61-73. doi:10.1111/j.1469-8137.2011.03793.x. PubMed: 21692804.
71. Yamaguchi-Shinozaki K, Shinozaki K, (2005) Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends Plant Sci 10: 88-94. doi:10.5363/tits.10.10_88. PubMed: 15708346.
72. Shinozaki K, Yamaguchi-Shinozaki K, (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3: 217-223. doi:10.1016/S1369-5266(00)00067-4. PubMed: 10837265.
73. Pical C, Fredlund KM, Petit PX, Sommarin M, Møller IM, (1993) The outer membrane of plant mitochondria contains a calcium-dependent protein kinase and multiple phosphoproteins. FEBS Lett 336: 347-351. doi:10.1016/0014-5793(93)80835-I. PubMed: 8262260.
74. Anil VS, Harmon AC, Rao KS, (2000) Spatio-temporal accumulation and activity of calcium-dependent protein kinases during embryogenesis, seed development, and germination in sandalwood. Plant Physiol 122: 1035-1043. doi:10.1104/pp.122.4.1035. PubMed: 10759499.
75. Rutschmann F, Stalder U, Piotrowski M, Oecking C, Schaller A, (2002) LeCPK1, a calcium-dependent protein kinase from tomato. Plasma membrane targeting and biochemical characterization. Plant Physiol 129: 156-168. doi:10.1104/pp.000869. PubMed: 12011347.
76. Rodriguez Milla MA, Uno Y, Chang IF, Townsend J, Maher EA, et al. (2006) A novel yeast two-hybrid approach to identify CDPK substrates: characterization of the interaction between AtCPK11 and AtDi19, a nuclear zinc finger protein. FEBS Lett 580: 904-911. doi:10.1016/j.febslet.2006.01.013. PubMed: 16438971.
77. Patharkar OR, Cushman JC, (2006) A novel coiled-coil protein co-localizes and interacts with a calcium-dependent protein kinase in the common ice plant during low-humidity stress. Planta 225: 57-73. doi:10.1007/s00425-006-0330-0. PubMed: 16773372.
78. Pareek A, Sopory SK, Bohnert HJ, (2010) Abiotic Stress Adaptation in Plants: Physiological, Molecular and Genomic Foundation. Springer Verlag.
79. Agarwal PK, Agarwal P, Reddy MK, Sopory SK, (2006) Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Rep 25: 1263-1274. doi:10.1007/s00299-006-0204-8. PubMed: 16858552.
80. Shinozaki K, Yamaguchi-Shinozaki K, (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58: 221-227. PubMed: 17075077.
81. Nakashima K, Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K, (1997) A nuclear gene, erd1, encoding a chloroplast-targeted Clp protease regulatory subunit homolog is not only induced by water stress but also developmentally up-regulated during senescence in Arabidopsis thaliana. Plant J 12: 851-861. doi:10.1046/j.1365-313X.1997.12040851.x. PubMed: 9375397.
82. Silva EN, Ferreira-Silva SL, Fontenele Ade V, Ribeiro RV, Viégas RA, et al. (2010) Photosynthetic changes and protective mechanisms against oxidative damage subjected to isolated and combined drought and heat stresses in Jatropha curcas plants. J Plant Physiol 167: 1157-1164. doi:10.1016/j.jplph.2010.03.005. PubMed: 20417989.