In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS

Lecture Notes in Engineering and Computer Science

Volumn 2197, Issue , 2012, Pages 572-577

  Erra Balsells, Rosa ^a   Gholipour, Yousef ^b   Nonami, Hiroshi ^b  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b EHIME UNIVERSITY   (Japan)

Author keywords

Metabolomics; Nanoparticles; Plant cell; Shotgun metabolite profiling; UV MALDI TOF

Indexed keywords

BIOMOLECULES; CELL MEMBRANES; DESORPTION; MASS SPECTROMETRY; METABOLITES; NANOPARTICLES; PLANT CELL CULTURE; PLANTS (BOTANY); POTENTIAL FLOW; PROBES;

MALDI-TOF; MALDI-TOF MASS SPECTROMETRY; MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME OF FLIGHTS; MEMBRANE PROPERTIES; METABOLITE ANALYSIS; METABOLITE PROFILING; METABOLOMICS; PLANT CELLS;

CYTOLOGY;

EID: 84885917603     PISSN: 20780958     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (14)

1. A. Amantonico, P. L. Urban and R. Zenobi, “Analytical techniques for single-cell metabolomics: state of the art and trends,” Anal. Bioanal. Chem., vol. 398, pp. 2493-2504. 2010.
2. A. Avatoš, “Single-cell metabolomics comes of age: new developments in mass spectrometry profiling and imaging.” Anal. Chem., vol. 83, pp. 5037-5044, 2011.
3. A. Amantonico, J. Y. Oh, J. Sobek, M. Heinemann, and R. Zenobi, “Mass spectrometric method for analyzing metabolites in yeast with single cell sensitivity,” Angew. Chem. Int. Ed., vol. 47, pp. 5382-5385, 2008.
4. B. Ebert, D. Zöller, A. Erban, I. Fehrle, J. Hartmann, A. Niehl, J. Kopka, and J. Fisahn, “Metabolic profiling of Arabidopsis thaliana epidermal cells,”J. Exp. Bot., vol. 61, pp. 1321-1335, 2010.
5. M. M. Koek, F. Bakels, W. Engel, A. van den Maagdenberg, M. D. Ferrari, L. Coulier, and T. Hankemeier, “Metabolic profiling of ultrasmall sample volumes with GC/MS: from microliter to nanoliter samples,” Anal. Chem., 82, pp. 156-162, 2010.
6. H. Nonami and J.S. Boyer, “Wall extensibility and cell hydraulic conductivity decrease in enlarging stem tissues at low water potentials,” Plant Physiol., vol. 93, pp. 1610-1619, 1990.
7. M. Malone, and A.D. Tomos, “A simple pressure-probe method for the determination of volume in higher-plant cells,” Planta, vol. 182, pp.199-203, 1990.
8. H. Nonami and E.-D. Schulze, “Cell water potential, osmotic potential, and turgor in the epidermis and mesophyll of transpiring leaves: Combined measurements with the cell pressure probe and nanoliter osmometer,” Planta vol. 177, pp.35-46, 1989.
9. H. Nonami and J.S. Boyer, “Direct demonstration of a growth-induced water potential gradient,” Plant Physiol. vol. 102, pp.13-19, 1993.
10. Y. Gholipour, H. Nonami and R. Erra-Balsells, “Application of pressure probe and UV-MALDI-TOF MS for direct analysis of plant underivatized carbohydrates in subpicoliter single-cell cytoplasm extract,” J. Am. Soc. Mass Spectrom., vol. 19, pp. 1841-1848, 2008.
11. H. Nonami, S. Fukui, and R. Erra-Balsells, “-Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: a comparative study using phenylcarbonyl compounds, carbazoles and classical matrices,” J. Mass Spectrom., vol. 32, pp. 287-296, 1997.
12. Y. Gholipour, H. Nonami, R. Erra-Balsells, “In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix,” Anal. Biochem., vol. 383, pp. 159-167, 2008.
13. Y. Gholipour, S.L. Giudicessi, H. Nonami and R. Erra-Balsells, “Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues,” Anal. Chem., vol. 82, pp. 5518-5526, 2010.
14. Z. Yu, L.C. Chen, H. Suzuki, O. Ariyada, R. Erra-Balsells, H. Nonami and K. Hiraoka, “Direct profiling of phytochemicals in tulip tissues and in vivo monitoring of the change of carbohydrate content in tulip bulbs by probe electrospray ionization mass spectrometry,” J. Amer. Soc. Mass Spectrom., vol. 20, pp. 2304-2311, 2009.