Delayed times to tissue fixation result in unpredictable global phosphoproteome changes

Journal of Proteome Research

Volumn 12, Issue 10, 2013, Pages 4424-4434

  Gündisch, Sibylle ^a,e   Grundner Culemann, Kathrin ^b,e   Wolff, Claudia ^a,e   Schott, Christina ^a,d   Reischauer, Bilge ^a,d   MacHatti, Manuela ^b,e   Groelz, Daniel ^c,d   Schaab, Christoph ^b,f   Tebbe, Andreas ^b,e   Becker, Karl Friedrich ^a,d,e  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b Evotec (Munich) GmbH   (Germany)

^c PreAnalytiX   (Switzerland)

^d QIAGEN GMBH   (Germany)

^e BioM Biotech Cluster Development GmbH   (Germany)

^f MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

biobank; biomarker; mass spectrometry; phosphoproteomics; preanalytical phase; protein; RPPA; tissue

Indexed keywords

CYTOKERATIN 18; PHOSPHOPROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; COLD ISCHEMIA; CRYOPRESERVATION; FREEZING; LIQUID CHROMATOGRAPHY; LIVER; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN MICROARRAY; PROTEIN PHOSPHORYLATION; PROTEOMICS; QUANTITATIVE ANALYSIS; RAT; REVERSE PHASE PROTEIN ARRAY; TANDEM MASS SPECTROMETRY; TISSUE FIXATION;

AMINO ACID SEQUENCE; ANIMALS; CELL HYPOXIA; CELL LINE, TUMOR; CHROMATOGRAPHY, REVERSE-PHASE; CRYOPRESERVATION; LIVER; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN KINASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOME; RATS; RATS, WISTAR; REFERENCE STANDARDS; TANDEM MASS SPECTROMETRY; TIME FACTORS; TISSUE FIXATION;

EID: 84885199044     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr400451z     Document Type: Article

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