A guide to design and optimization of reporter assays for 3' untranslated region mediated regulation of mammalian messenger RNAs

Methods

Volumn 63, Issue 2, 2013, Pages 110-118

  Van Etten, Jamie ^a   Schagat, Trista L ^a,b   Goldstrohm, Aaron C ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b PROMEGA CORPORATION   (United States)

Author keywords

Gene regulation; Luciferase; Messenger RNA

Indexed keywords

LUCIFERASE; MESSENGER RNA; RNA BINDING PROTEIN;

3' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; GENE EXPRESSION REGULATION; GENOME; HUMAN; HUMAN CELL; MAMMAL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; QUANTITATIVE ANALYSIS; REGULATORY MECHANISM; REVERSE TRANSCRIPTION; RNA PROCESSING; RNA PURIFICATION; RNA SEQUENCE; RNA TRANSLATION; TRANSCRIPTION REGULATION;

MAMMALIA;

GENE REGULATION; LUCIFERASE; MESSENGER RNA;

3' UNTRANSLATED REGIONS; ANIMALS; CLONING, MOLECULAR; GENES, REPORTER; HEK293 CELLS; HUMANS; LUCIFERASES, FIREFLY; LUCIFERASES, RENILLA; PLASMIDS; REAL-TIME POLYMERASE CHAIN REACTION; RESPONSE ELEMENTS; RNA, MESSENGER; TRANSFECTION;

EID: 84885087303     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2013.04.020     Document Type: Article

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