Multicolor flow cytometric analysis of immune cell subsets in tumor-bearing mice

Cold Spring Harbor Protocols

Volumn 2013, Issue 10, 2013, Pages 955-960

  Bayne, Lauren J ^a   Vonderheide, Robert H ^a,b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD11B ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN;

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; BONE MARROW CELL; CELL POPULATION; CELL SUSPENSION; DENDRITIC CELL; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; IMMUNOCOMPETENT CELL; LEUKOCYTE; MOUSE; NATURAL KILLER CELL; NEOPLASM; NONHUMAN; PROTEIN EXPRESSION; SPLEEN CELL; T LYMPHOCYTE;

ANIMALS; COLOR; CYTOLOGICAL TECHNIQUES; FLOW CYTOMETRY; LEUKOCYTES; MICE; NEOPLASMS; STAINING AND LABELING;

EID: 84885054281     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot077198     Document Type: Article

References (7)

1. Bayne LJ, Beatty GL, Jhala N, Clark CE, Rhim AD, Stanger BZ, Vonderheide RH. 2012. Tumor-derived granulocyte-macrophage colony-stimulating factor regulates myeloid inflammation and T cell immunity in pancreatic cancer. Cancer Cell 21: 822-835.
2. Bayne LJ, Vonderheide RH. 2013a. Immunohistochemical assessment of immune cells in mouse tumor tissue. Cold Spring Harb Protoc doi: 10.1101/pdb.prot077206.
3. Bayne LJ, Vonderheide RH. 2013b. A myeloid-derived suppressor cell-mediated T-cell suppression assay for functional evaluation of immune cells in tumor-bearing mice. Cold Spring Harb Protoc doi: 10.1101/ pdb.prot077214.
4. Bayne LJ, Vonderheide RH. 2013c. Methods for analysis of the immune system in mouse cancer models. Cold Spring Harb Protoc doi: 10.1101/pdb.top069971.
5. Beatty GL, Chiorean EG, Fishman MP, Saboury B, Teitelbaum UR, Sun W, Huhn RD, Song W, Li D, Sharp LL, et al. 2011. CD40 agonists alter tumor stroma and show efficacy against pancreatic carcinoma in mice and humans. Science 331: 1612-1616.
6. Clark CE, Hingorani SR, Mick R, Combs C, Tuveson DA, Vonderheide RH. 2007. Dynamics of the immune reaction to pancreatic cancer from inception to invasion. Cancer Res 67: 9518-9527.
7. Stairs DB, Bayne LJ, Rhoades B, Vega ME, Waldron TJ, Kalabis J, Klein Szanto A, Lee JS, Katz JP, Diehl JA, et al. 2011. Deletion of p120-catenin results in a tumor microenvironment with inflammation and cancer that establishes it as a tumor suppressor gene. Cancer Cell 19: 470-483.