Comparative diagnostic and analytical performance of PCR and LAMP-based trypanosome detection methods estimated using pooled whole tsetse flies and midguts

Veterinary Parasitology

Volumn 197, Issue 3-4, 2013, Pages 549-556

  Malele, Imna I ^a   Ouma, Johnson O ^b   Enyaru, John C K ^c   Matovu, Enock ^c   Alibu, Vincent ^c   Auma, Joanna E ^b   Onyoyo, Samuel G ^b   Bateta, Rosemary ^b   Changasi, Robert E ^b   Mukiria, Phoebe W ^b   Ndung'u, Kariuki ^b   Gitonga, Purity K ^b   Mwaniki, Lilian M ^b   Nyingilili, Hamisi S ^a   Lyaruu, Eugene A ^a   Kapange, Lupakisyo A ^a   Kamau, Peter Kuria ^d   Masiga, Daniel K ^d  

^a Tsetse and Trypanosomiasis Research Institute   (Tanzania)

^b KENYA AGRICULTURAL RESEARCH INSTITUTE   (Kenya)

^c MAKERERE UNIVERSITY   (Uganda)

^d INTERNATIONAL CENTRE OF INSECT PHYSIOLOGY AND ECOLOGY ICIPE   (Kenya)

Author keywords

LAMP; PCR; Pooling; Trypanosomes; Tsetse; Xenomonitoring

Indexed keywords

INTERNAL TRANSCRIBED SPACER; PROTOZOAL DNA;

AFRICAN TRYPANOSOMIASIS; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; DNA DETERMINATION; GENE SEQUENCE; GENE TARGETING; GLOSSINIDAE; ITS POLYMERASE CHAIN REACTION; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MIDGUT; NONHUMAN; PARASITE EXAMINATION; POLYMERASE CHAIN REACTION; REPETITIVE INSERTION MOBILE ELEMENT; TBR POLYMERASE CHAIN REACTION; TRYPANOSOMA BRUCEI;

GLOSSINA (GENUS); TRYPANOSOMA BRUCEI BRUCEI; TRYPANOZOON;

LAMP; PCR; POOLING; TRYPANOSOMES; TSETSE; XENOMONITORING;

ANIMALS; DIGESTIVE SYSTEM; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRYPANOSOMA; TSETSE FLIES;

EID: 84884984671     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2013.05.022     Document Type: Article

References (20)

1. Adams E.R., Hamilton P.B., Malele I.I., Gibson W.C. The identification, diversity and prevalence of trypanosomes in field caught tsetse in Tanzania using ITS-1 primers and fluorescent fragment length barcoding. Infect. Genet. Evol. 2008, 8:439-444.
2. Basompem K.M., Masake R.A., Assoku R.K., Opiyo E.A., Nantulya V.M. Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.). Parasitology 1996, 112(Pt 2):205-211.
3. Bhattacharya S., Bakre A., Bhattacharya A. Mobile genetic elements in protozoan parasites. J. Genet. 2002, 81:73-86.
4. Cox A., Tilley A., McOdimba F., Fyfe J., Eisler M., Hide G., Welburn S. A PCR based assay for detection and differentiation of African trypanosome species in blood?. Exp. Parasitol. 2005, 111(1):24-29.
5. Dale C., Welburn S.C., Maudlin I., Milligan P.J. The kinetics of maturation of trypanosome infections in tsetse. Parasitology 1995, 111(Pt 2):187-191.
6. Desquesnes M., Davila A.M. Applications of PCR-based tools for the detection and identification of animal trypanosomes: a review and perspectives. Vet. Parasitol. 2002, 109(3-4):213-231.
7. Enyaru J.C., Ouma J.O., Malele I.I., Matovu E., Masiga D.K. Landmarks in the evolution of technologies for identifying trypanosomes in tsetse flies. Trends Parasitol. 2010, 20.26:388-394.
8. Ferreira F., Cano J., Furtado A., Ndong-Mabale N., Ndong-Asumu P., et al. An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection. Journal of Infection in Developing Countries 2008, 2:63-67.
9. Lehane M.J., Msangi A.R., Whitaker C.J., Lehane S.M. Grouping of trypanosome species in mixed infections in Glossina pallidipes. Parasitology 2000, 120:583-592.
10. Lloyd L., Johnson W.B. The trypanosome infections of tsetse flies in northern Nigeria and a new method of estimation. Bull. Entomol. Res. 1924, 14:265-288.
11. Malele I., Craske L., Knight C., Ferris V., Njiru Z., Hamilton P., Lehane S., Lehane M., Gibson W. The use of specific and generic primers to identify trypanosome infections of wild tsetse flies in Tanzania by PCR. Infect. Genet. Evol. 2003, 3:271-279.
12. Milligan P.J., Maudlin I., Welburn S.C. Trypanozoon: infectivity to humans is linked to reduced transmissibility in tsetse. II. Genetic mechanisms. Exp. Parasitol. 1995, 81:409-415.
13. Morlais I., Grebaut P., Bodo J.M., Djoha S., Cuny G., Herder S. Detection and identification of trypanosomes by polymerase chain reaction in wild tsetse flies in Cameroon. Acta Trop. 1998, 70:109-117.
14. Moser D.R., Cook G.A., Ochs D.E., Bailey C.P., McKane M.R., Donelson J.E. Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction. Parasitology 1989, 99(Pt 1):57-66.
15. Njiru Z.K., Makumi J.N., Okoth S., Ndungu J.M., Gibson W.C. Identification of trypanosomes in Glossina pallidipes and G. longipennis in Kenya. Infect. Genet. Evol. 2004, 4:29-35.
16. Njiru Z.K., Constantine C.C., Guya S., Crowther J., Kiragu J.M., Thompson R.C.A., Davila A. The use of ITS1 rDNA PCR in detecting pathogenic African trypanosomes. Parasitol. Res. 2005, 95:186-192.
17. Njiru Z.K., Mikosza A.S.J., Matovu E., Enyaru J.C.K., Ouma J.O., Kibona S.N., Thompson R.C.A., Ndung'u J.M. African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA. Int. J. Parasitol. 2008, 38:589-599.
18. Okoth J.O., Kapaata R. Trypanosome infection rates in Glossina fuscipes fuscipes Newst. in the Busoga sleeping sickness focus, Uganda. Ann. Trop. Med. Parasitol. 1986, 80:459-461.
19. Ouma J.O., Masake R.A., Masiga D.K., Moloo S.K., Njuguna J.T., Ndung'u J.M. Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: glossinidae). Acta Trop. 2000, 75:315-321.
20. Welburn S.C., Maudlin I., Milligan P.J. Trypanozoon: infectivity to humans is linked to reduced transmissibility in tsetse. I. Comparison of human serum-resistant and human serum-sensitive field isolates. Exp. Parasitol. 1995, 81:404-408.