High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis

Cell Biology, Four-Volume Set

Volumn 4, Issue , 2006, Pages 175-188

  Görg, Angelika ^a   Weiss, Walter ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84884858086     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012164730-8/50208-2     Document Type: Chapter

References (23)

1. Bjellqvist B., Ek K., Righetti P.G., Gianazza E., Görg A., Westermeier R., Postel W. Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications. J. Biochem. Biophys. Methods 1982, 6:317-339.
2. Blomberg A., Blomberg L., Norbeck J., Fey S., Mose Larsen P., Larsen M., Roepstorff P., Degand H., Boutry M., Posch A., Görg A. Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two-dimensional gel electrophoresis. Electrophoresis 1995, 16:1935-1945.
3. Bossi A., Righetti P.G., Vecchio G., Severinsen S. Focusing of alkaline proteases in pH 10-12 immobilized gradients. Electrophoresis 1994, 15:1535-1540.
4. Corbett J.M., Dunn M.J., Posch A., Gorg A. Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: An interlaboratory comparison. Electrophoresis 1994, 15:1205-1211.
5. Görg A., Boguth G., Obermaier C., Posch A., Weiss W. Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): The state of the art and the controversy of vertical versus horizontal systems. Electrophoresis 1995, 16:1079-1086.
6. Görg A., Boguth G., Obermaier C., Weiss W. Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient. Electrophoresis 1998, 19:1516-1519.
7. Görg A., Obermaier C., Boguth G., Csordas A., Diaz J.J., Madjar J.J. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins. Electrophoresis 1997, 18:328-337.
8. Görg A., Obermaier C., Boguth G., Harder A., Scheibe B., Wildgruber R., Weiss W. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 2000, 21:1037-1053.
9. Görg A., Obermaier C., Boguth G., Weiss W. Recent developments in 2-D gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures. Electrophoresis 1999, 20:712-717.
10. Görg A., Postel W., Friedrich C., Kuick R., Strahler J.R., Hanash S.M. Electrophoresis 1991, 12:653-658.
11. Görg A., Postel W., Günther S. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 1988, 9:531-546.
12. Görg A., Weiss 2D electrophoresis with immobilized pH gradients. Proteome Research: Two Dimensional Electrophoresis and Identification Methods 2000, 57-106. Springer, Berlin. T. Rabilloud (Ed.).
13. Herbert B. Advances in protein solubilisation for two-dimensional electrophoresis. Electrophoresis 1999, 20:660-663.
14. Islam R., Ko C., Landers T. A new approach to rapid immobilised pH gradient IEF for 2-D electrophoresis. Sci. Tools 1998, 3:14-15.
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
16. O'Farrell P.H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 1975, 250:4007-4021.
17. O'Farrell P.Z., Goodman H.M., O'Farrell P.H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 1977, 12:1133-1142.
18. Olsson I., Larsson K., Palmgren R., Bjellqvist B. Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range IPG strips as first dimension. Proteomics 2002, 2:1630-1632.
19. Rabilloud T., Adessi C., Giraudel A., Lunardi J. Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 1997, 18:307-316.
20. Rabilloud T., Valette C., Lawrence J.J. Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH-gradients in the first dimension. Electrophoresis 1994, 15:1552-1558.
21. Righetti P.G. Immobilized pH Gradients: Theory and Methodology 1990, Elsevier, Amsterdam.
22. Wildgruber R., Harder A., Obermaier C., Boguth G., Weiss W., Larsen P.M., Fey S., Görg A. Towards higher resolution: Two-dimensional electrophoresis of Saccharomyces cerevisiae proteins using overlapping narrow immobilized pH gradients. Electrophoresis 2000, 21:2610-2616.
23. Wildgruber R., Reil G., Drews O., Parlar H., Görg A. Web-based two-dimensional database of Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Proteomics 2002, 2:727-732.