Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interactions: Applications in the Analysis of Mitochondrial Oxidative Phosphorylation Complexes

Cell Biology, Four-Volume Set

Volumn 4, Issue , 2006, Pages 259-264

  Smet, Joél ^b   Devreese, Bart ^a   Beeumen, Jozef Van ^a   Van Coster, Rudy N A ^b  

^a GHENT UNIVERSITY   (Belgium)

^b GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84884856036     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012164730-8/50217-3     Document Type: Chapter

References (8)

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2. Devreese B., Vanrobaeys F., Smet J., Van Beeumen J., Van Coster R. Mass spectrometric identification of mitochondrial oxidative phosphorylation subunits separated by two-dimensional blue-native polyacrylamide gel electrophoresis. Electrophoresis 2002, 23(15):2525-2533.
3. Schägger H. Quantification of oxidative phosphorylation enzymes after blue native electrophoresis and two-dimensional resolution: Normal complex I protein amounts in Parkinson's disease conflict with reduced catalytic activities. Electrophoresis 1995, 16(5):763-770.
4. Schägger H., von Jagow G. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 1991, 199(2):223-231.
5. Scholte H.R., Ross J.D., Blow W., Boonman A.M., van Diggelen O.P., Hall C.L., Huijmans J.G., Luyt-Houwen I.E., Kleijer W.J., de Klerk J.B., et al. Assessment of deficiencies of fatty acyl-CoA dehydrogenases in fibroblasts, muscle and liver. J. Inherit. Metab. Dis. 1992, 15(3):347-352.
6. Towbin H., et al. Proc. Natl. Acad. Sci. USA 1979, 76:4350.
7. Van Coster R., Smet J., George E., De Meirleir L., Seneca S., Van Hove J., Sebire G., Verhelst H., De Bleecker J., Van Vlem B., Verloo P., Leroy J. Blue native polyacrylamide gel electrophoresis: A powerful tool in diagnosis of oxidative phosphorylation defects. Pediatr. Res. 2001, 50(5):658-665.
8. Zerbetto E., Vergani L., Dabbeni-Sala F. Quantification of muscle mitochondrial oxidative phosphorylation enzymes via histochemical staining of blue native polyacrylamide gels. Electrophoresis 1997, 18(11):2059-2064.