Cultivation and retroviral infection of human epidermal keratinocytes

Cell Biology, Four-Volume Set

Volumn 1, Issue , 2006, Pages 133-138

  Watt, Fiona M ^a   Simon, Broad ^a   Prowse, David M ^b  

^a London Research Institute   (United Kingdom)

^b QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84884848398     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012164730-8/50017-4     Document Type: Chapter

References (17)

1. Adams J.C., Watt F.M. An unusual strain of human keratinocytes which do not stratify or undergo terminal differentiation in culture. J. Cell Biol. 1988, 107:1927-1938.
2. Compton C.C., Gill J.M., Bradford D.A., Regauer S., Gallico G.G., O'Connor N.E. Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting: A light, electron microscopic and immunohistochemical study. Lab. Invest. 1989, 60:600-612.
3. Dajee M., Lazarov M., Zhang J.Y., Cai T., Green C.L., Russell A.J., Marinkovich M.P., Tao S., Lin Q., Kubo Y., Khavari P.A. NF-KB blockade and oncogenic Ras trigger invasive human epidermal neoplasia. Nature 2003, 421:639-643.
4. Dellambra E., Prislei S., Salvati A.L., Madeddu M.L., Golisano O., Siviero E., Bondanza S., Cicuzza S., Orecchia A., Giancotti F.G., Zambruno G., De Luca M. Gene correction of integrin [~4-dependent pyloric, atresia-junctional epidermolysis bullosa keratinocytes establishes, a role for β4 tyrosines 1422 and 1440 in hemidesmosome assembly. J Biol Chem. 2001, 276:41336-41342.
5. Gerrard A.J., Hudson D.L., Brownlee G.G., Watt F.M. Towards gene therapy for haemophilia B using primary human keratinocytes. Nature Genet. 1993, 3:180-183.
6. Jones P.H., Watt F.M. Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 1993, 73:713-724.
7. Levy L., Broad S., Diekmann D., Evans R.D., Watt F.M. 31 integrins regulate keratinocyte adhesion and differentiation by distinct mechanisms. Mol. Biol. Cell. 2000, 11:453-466.
8. Levy L., Broad S., Zhu A.J., Carroll J.M., Khazaal I., Péault B., Watt F.M. Optimised retroviral infection of human epidermal keratinocytes: Long-term expression of transduced integrin gene following grafting on to SCID mice. Gene Ther. 1998, 5:913-922.
9. Markowitz D., Goff S., Bank A. Construction and use of a safe and efficient amphotropic packaging cell line. Virology. 1988, 167:400-406.
10. Morgenstern J.P., Land H. Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 1990, 18:3587-3596.
11. Ortiz-Urda S., Thyagarajan B., Keene D.R., Lin Q., Fang M., Calos M.P., Khavari P.A. Stable nonviral genetic correction of inherited human skin disease. Nature Med. 2002, 8:1166-1170.
12. Rheinwald J.G. Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells. Cell Growth and Division: A Practical Approach 1989, 81-94. IRL Press, Oxford. R. Baserga (Ed.).
13. Rheinwald J.G., Green H. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell 1975, 6:331-343.
14. Ronfard V., Rives J.M., Neveux Y., Carsin H., Barrandon Y. Long-term regeneration of human epidermis on third degree burns transplanted with autologous cultured epithelium grown on a fibrin matrix. Transplantation 2000, 15:1588-1598.
15. Swift S.E., Lorens J.B., Achacoso P., Nolan G.P. Rapid production of retrovirus for efficient gene delivery to mammalian cells using 293T cell-based systems. Current Protocols in Immunology 1999, Vol 1. 17C:1-17. Wiley, New York. J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, W. Strober (Eds.).
16. Zhu A.J., Watt F.M. Expression of a dominant negative cadherin mutant inhibits proliferation and stimulates terminal differentiation of human epidermal keratinocytes. J. Cell Sci. 1996, 109:3013-3023.
17. Zhu A.J., Watt F.M. β-Catenin signalling modulates proliferative potential of human epidermal keratinocytes independently of intercellular adhesion. Development 1999, 126:2285-2298.