Deciphering activation of olfactory receptors using heterologous expression in saccharomyces cerevisiae and bioluminescence resonance energy transfer

Methods in Molecular Biology

Volumn 1003, Issue , 2013, Pages 149-160

  Sanz, Guenhaël ^a   Pajot Augy, Edith ^a  

^a INRA   (France)

Author keywords

BRET; GPCR; Heterologous expression; Homodimerization; Olfactory receptor

Indexed keywords

DIMER; LIGAND;

ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL MEMBRANE; CONFORMATIONAL TRANSITION; EXPRESSION VECTOR; FUNGAL CELL CULTURE; HETEROLOGOUS EXPRESSION; IMMUNOBLOTTING; LIGAND BINDING; NONHUMAN; ODOR; OLFACTORY RECEPTOR; OLIGOMERIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; STIMULATION; ARTIFICIAL MEMBRANE; CHEMISTRY; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PLASMID; PROTEIN MULTIMERIZATION; PROTEIN QUATERNARY STRUCTURE;

BIOLUMINESCENCE RESONANCE ENERGY TRANSFER TECHNIQUES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GENETIC ENGINEERING; HUMANS; IMMUNOBLOTTING; LIGANDS; MEMBRANES, ARTIFICIAL; PLASMIDS; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; RECEPTORS, ODORANT; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 84884780109     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-377-0_11     Document Type: Article

References (10)

1. Hague C, Uberti MA, Chen Z, Bush CF, Jones SV, Ressler KJ, Hall RA, Minneman KP (2004) Olfactory receptor surface expression is driven by association with the beta2-adrenergic receptor. Proc Natl Acad Sci USA 101: 13672-13676
2. Bush CF, Jones SV, Lyle AN, Minneman KP, Ressler KJ, Hall RA (2007) Speci fi city of olfactory receptor interactions with other G protein-coupled receptors. J Biol Chem 282: 19042-19051
3. Saito H, Kubota M, Roberts RW, Chi Q, Matsunami H (2004) RTP family members induce functional expression of mammalian odorant receptors. Cell 119:679-691
4. Wade F, Espagne A, Persuy MA, Vidic J, Monnerie R, Merola F, Pajot-Augy E, Sanz G (2011) Relationship between homo-oligomerization of a mammalian olfactory receptor and its activation state demonstrated by bioluminescence resonance energy transfer. J Biol Chem 286:15252-15259
5. Milligan G, Bouvier M (2005) Methods to monitor the quaternary structure of G proteincoupled receptors. FEBS J 272:2914-2925
6. Gandia J, Lluis C, Ferre S, Franco R, Ciruela F (2008) Light resonance energy transfer-based methods in the study of G protein- coupled receptor oligomerization. Bioessays 30: 82-89
7. Bacart J, Corbel C, Jockers R, Bach S, Couturier C (2008) The BRET technology and its application to screening assays. Biotechnol J 3:311-324
8. Gehret AU, Bajaj A, Naider F, Dumont ME (2006) Oligomerization of the yeast alpha-factor receptor: implication for dominant negative effects of mutant receptors. J Biol Chem 281:20698-20714
9. Vidic J, Grosclaude J, Monnerie R, Persuy MA, Badonnel K, Baly C, Caillol M, Briand L, Salesse R, Pajot-Augy E (2008) On a chip demonstration of a functional role for odorant binding protein in the preservation of olfactory receptor activity at high odorant concentration. Lab Chip 8:678-688
10. Crowe ML, Perry BN, Connerton IF (2000) Golf complements a GPA1 null mutation in Saccharomyces cerevisiae and functionally couples to the STE2 pheromone receptor. J Recept Signal Transduct Res 20:61-73