Mycobacterium tuberculosis detection in blood using multiplex nested polymerase chain reaction

International Journal of Tuberculosis and Lung Disease

Volumn 17, Issue 10, 2013, Pages 1341-1345

  Dubey, Alka ^a   Gwal, R ^a   Agrawal, S ^a  

^a Cairn India Ltd   (India)

Author keywords

Blacklight card; Blood TB PCR; IS6110; MPB64 sequence

Indexed keywords

BACTERIAL DNA;

ADOLESCENT; ADULT; AGED; ARTICLE; BACTERIAL GENOME; BACTERIUM DETECTION; BLOOD ANALYSIS; CHILD; CONTROLLED STUDY; CORYNEBACTERIUM DIPHTHERIAE; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; EXTRAPULMONARY TUBERCULOSIS; FEMALE; GENE AMPLIFICATION; GENE INSERTION; GENE SEQUENCE; HUMAN; MAJOR CLINICAL STUDY; MALE; MILIARY TUBERCULOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOBACTERIUM TUBERCULOSIS; NOCARDIA; PERIPHERAL BLOOD MONONUCLEAR CELL; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRESCHOOL CHILD; PRIORITY JOURNAL; RETROSPECTIVE STUDY; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; STANDARDIZATION; TUBERCULOSIS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHILD; CHILD, PRESCHOOL; FEMALE; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MIDDLE AGED; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TUBERCULOSIS; YOUNG ADULT;

EID: 84884598377     PISSN: 10273719     EISSN: None     Source Type: Journal    
DOI: 10.5588/ijtld.12.0536     Document Type: Article

References (16)

1. World Health Organization. WHO global TB report 2010. WHO/HTM/TB/2010.7. Geneva, Switzerland: WHO, 2010.
2. Figueirêdo da Costa Lima J, Montenegro L M L, de Albuquerque Montenegro R, et al. Performance of nested PCR in the specific detection of Mycobacterium tuberculosis complex in blood samples of pediatric patients. J Bras Pneumol 2009; 690-697.35:.
3. Paramasivan C, Narayana A, Prabhakar R. Effect of storage of sputum specimens at room temperature on smear and culture results. Tubercle 1983; 64: 119-124.
4. Guio H, Okayama H, Ashino Y, et al. Method for effi cient storage and transportation of sputum specimens for molecular testing of tuberculosis. Int J Tuberc Lung Dis 2006; 10: 906- 910.
5. Nandagopal B, Sankar S, Lingesan K, Appu K C, Sridharan G, Gopinathan A K. Evaluation of a nested PCR targeting IS6110 of Mycobacterium tuberculosis for detection of the organism in the leukocyte fraction of blood samples. Indian J Med Microbiol 2010; 28: 227-232.
6. World Health Organization. Commercial serodiagnostic tests for diagnosis of tuberculosis: policy statement. WHO/HTM/TB/2011.5. Geneva, Switzerland: WHO, 2011.
7. Kant L. Improving detection of infectious cases. Indian J Tuberc 2001; 48: 115.
8. Pahwa R, Hedau S, Jain S, Jain N, Arora V M, Kumar N, Das B C. Assessment of possible tuberculous lymphadenopathy by PCR compared to non-molecular methods. J Med Microbiol 2005; 54: 873.
9. Shukla I, Varshney S, Sarfraz, Malik A, Ahmad Z. Evaluation of nested PCR targeting IS6110 of Mycobacterium tuberculosis for the diagnosis of pulmonary and extra-pulmonary tuberculosis. Biol Med 2011; 3: 171-175.
10. Tansuphasiril U, Chinrat B, Rienthong S. Evaluation of culture and PCR-based assay for detection of Mycobacterium tuberculosis from sputum collected and stored on filter paper. J Med Assoc Thailand 2001; 32: 844-855.
11. Katoch V M. Newer diagnostic techniques for tuberculosis. Indian J Med Res 2004; 120: 418-428.
12. Khan M A, Mirza S H, Abbasi S A, Butt T, Anwar M. Peripheral blood-based polymerase chain reaction in diagnosis of pulmonary tuberculosis. J Ayub Med Coll Abbottabad 2006; 18: 25-28.
13. Iqbal S, Amin D, Mumtaz A, Shabir I. Signifi cance of appropriate sampling in the diagnosis of tuberculosis (TB)-a comparison of different techniques. Biomedica 2010; 26: 39-44.
14. Ahmed N, Mohanty A K, Mukhopadhyay U, Batish V K, Grover S. PCR-based rapid detection of Mycobacterium tuberculosis in blood from immunocompetent patients with pulmonary tuberculosis. J Clin Microbiol 1998; 36: 3094-3095.
15. Indulakshmi R, Manju Y K, Kumar R A, Mundayoor S. Implications of low frequency of IS6110 in fingerprinting field isolates of Mycobacterium tuberculosis from Kerala, India. J Clin Microbiol 2001; 39: 1683.
16. Narayanan S, Das S, Garg R, et al. Molecular epidemiology of tuberculosis in a rural area of high prevalence in South India: implications for disease control and prevention. J Clin Microbiol 2002; 40: 4785-4788.