In vitro transcription from plasmid or PCR-amplified DNA

Methods in Enzymology

Volumn 530, Issue , 2013, Pages 101-114

  Brunelle, Julie L ^a   Green, Rachel ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Column purification; DNA; DNA Phenol extract; In vitro transcription reaction; PCR amplified; RNA polymerase; Template

Indexed keywords

PLASMID DNA;

ARTICLE; CHEMICAL MODIFICATION; DNA TEMPLATE; DNA TRANSCRIPTION; GENE AMPLIFICATION; IN VITRO STUDY; MOLECULAR SIZE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA PURIFICATION; RNA SYNTHESIS;

COLUMN PURIFICATION; IN VITRO TRANSCRIPTION REACTION; PCR-AMPLIFIED DNA; PHENOL EXTRACT DNA TEMPLATE; RNA POLYMERASE;

BACTERIOPHAGE T7; DNA; PHENOL; PLASMIDS; POLYMERASE CHAIN REACTION; RNA; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC;

EID: 84884268713     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420037-1.00005-1     Document Type: Chapter

References (6)

1. J.F. Milligan, and O.C. Uhlenbeck Synthesis of small RNAs using T7 RNA polymerase Methods in Enzymology 180 1989 51 62
2. J.F. Milligan, and O.C. Uhlenbeck Synthesis of small RNAs using T7 RNA polymerase Methods in Enzymology 180 1989 51 62
3. RNA purification by preparative polyacrylamide gel electrophoresis.
4. Explanatory chapter: PCR -Primer design.
5. Agarose Gel Electrophoresis.
6. Explanatory Chapter: Nucleic Acid Concentration Determination.