Safe pseudovirusbased assay for neutralization antibodies against influenza A(H7N9) virus

Emerging Infectious Diseases

Volumn 19, Issue 10, 2013, Pages 1685-1687

  Qiu, Chao ^a,b   Huang, Yang ^a,b   Zhang, Anli ^a,b   Tian, Di ^a   Wan, Yanmin ^a   Zhang, Xiaoling ^a   Zhang, Wanju ^a   Zhang, Zhiyong ^a   Yuan, Zhenghong ^a,b   Hu, Yunwen ^a   Zhang, Xiaoyan ^a,b,c   Xu, Jianqing ^a,b,c  

^a SHANGHAI PUBLIC HEALTH CLINICAL CENTER   (China)

^b FUDAN UNIVERSITY   (China)

^c CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

[No Author keywords available]

Indexed keywords

HEMAGGLUTININ; LUCIFERASE; NEUTRALIZING ANTIBODY; SIALIDASE; CAPSID PROTEIN;

ADULT; AGED; ARTICLE; CELL CULTURE; CLINICAL ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IC 50; IMMUNE SYSTEM; INFLUENZA A; INFLUENZA A (H7N9); NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEROLOGY; TISSUE CULTURE; VIRUS NEUTRALIZATION; BLOOD; CELL STRAIN HEK293; GENE VECTOR; GENETIC ENGINEERING; GENETICS; IMMUNOLOGY; INFLUENZA; INFLUENZA VIRUS A H7N9; LENTIVIRINAE; MIDDLE AGED; MOLECULAR CLONING; PSEUDOVIRUS; SERODIAGNOSIS; SUBTYPE H7N9; VERY ELDERLY; VIROLOGY; VIRUS; HEK293 CELL LINE; INFLUENZA A VIRUS (H7N9);

INFLUENZA; NEUTRALIZATION TESTS; PSEUDOVIRUS; SEROLOGY; SUBTYPE H7N9; VIRUSES;

AGED; AGED, 80 AND OVER; ANTIBODIES, NEUTRALIZING; CAPSID PROTEINS; CLONING, MOLECULAR; GENETIC ENGINEERING; GENETIC VECTORS; HEK293 CELLS; HUMANS; INFLUENZA A VIRUS, H7N9 SUBTYPE; INFLUENZA, HUMAN; LENTIVIRUS; MIDDLE AGED; NEUTRALIZATION TESTS;

EID: 84884257708     PISSN: 10806040     EISSN: 10806059     Source Type: Journal    
DOI: 10.3201/eid1910.130728     Document Type: Article

References (7)

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6. Labrosse B, Tourdjman M, Porcher R, LeGoff J, de Lamballerie X, Simon F, et al. Detection of extensive cross-neutralization between pandemic and seasonal A/H1N1 influenza viruses using a pseudotype neutralization assay. PLoS ONE. 2010;5:e11036. http://dx.doi. org/10.1371/journal.pone.0011036
7. Nefkens I, Garcia JM, Ling CS, Lagarde N, Nicholls J, Tang DJ, et al. Hemagglutinin pseudotyped lentiviral particles: characterization of a new method for avian H5N1 influenza sero-diagnosis. J Clin Virol. 2007;39:27-33. http://dx.doi.org/10.1016/j.jcv.2007.02.005