Chondrogenic effect of precartilaginous stem cells following NLS-TAT cell penetrating peptide-assisted transfection of eukaryotic hTGFβ3

Journal of Cellular Biochemistry

Volumn 114, Issue 11, 2013, Pages 2588-2594

  Guo, Xin ^a   Chu, Xiangyu ^a   Li, Wenkai ^a   Pan, Qiyong ^a   You, Hongbo ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Cell Penetrating Peptide; Chondrogenesis; Peptide Based Carriers; Precartilaginous Stem Cells

Indexed keywords

AGGRECAN; CELL PENETRATING PEPTIDE; COLLAGEN TYPE 2; DNA; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR SOX9; TRANSFORMING GROWTH FACTOR BETA3;

ANIMAL CELL; ARTICLE; CARTILAGE INJURY; CHONDROGENESIS; CONTROLLED STUDY; GENE; GENETIC TRANSFECTION; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; NUCLEOTIDE SEQUENCE; PRECARTILAGINOUS STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; STEM CELL;

CELL PENETRATING PEPTIDE; CHONDROGENESIS; PEPTIDE-BASED CARRIERS; PRECARTILAGINOUS STEM CELLS;

ANIMALS; BLOTTING, WESTERN; CELLS, CULTURED; CHONDROGENESIS; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; NUCLEAR LOCALIZATION SIGNALS; RATS; RATS, SPRAGUE-DAWLEY; REAL-TIME POLYMERASE CHAIN REACTION; STEM CELLS; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA3;

EID: 84884222595     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.24606     Document Type: Article

References (22)

1. Brasseur R, Divita G,. 2010. Happy birthday cell penetrating peptides: Already 20 years. Biochim Biophys Acta 1798: 2177-2181.
2. Chen CP, Chou JC, Liu BR, Chang M, Lee HJ,. 2007. Transfection and expression of plasmid DNA in plant cells by an arginine-rich intracellular delivery peptide without protoplast preparation. FEBS Lett 581: 1891-1897.
3. Choi YS, Lim SM, Shin HC, Lee CW, Kim SL, Kim DI,. 2007. Chondrogenesis of human periosteum-derived progenitor cells in atelocollagen. Biotechnol Lett 29: 323-329.
4. Davis PB, Cooper MJ,. 2007. Vectors for airway gene delivery. AAPS J 9: E11-E17.
5. Eguchi A, Furusawa H, Yamamoto A, Akuta T, Hasegawa M, Okahata Y, Nakanishi M,. 2005. Optimization of nuclear localization signal for nuclear transport of DNA-encapsulating particles. J Control Release 104: 507-519.
6. Kwon HJ,. 2013. Chondrogenesis on sulfonate-coated hydrogels is regulated by their mechanical properties. J Mech Behav Biomed Mater 17: 337-346.
7. Lehto T, Simonson OE, Mager I, Ezzat K, Sork H, Copolovici DM, Viola JR, Zaghloul EM, Lundin P, Moreno PM, Mae M, Oskolkov N, Suhorutsenko J, Smith CI, Andaloussi SE,. 2011. A peptide-based vector for efficient gene transfer in vitro and in vivo. Mol Ther 19: 1457-1467.
8. Liu BR, Lin MD, Chiang HJ, Lee HJ,. 2012. Arginine-rich cell-penetrating peptides deliver gene into living human cells. Gene 505: 37-45.
9. Madani F, Lindberg S, Langel U, Futaki S, Graslund A,. 2011. Mechanisms of cellular uptake of cell-penetrating peptides. J Biophys 2011: 414729.
10. Margus H, Padari K, Pooga M,. 2012. Cell-penetrating peptides as versatile vehicles for oligonucleotide delivery. Mol Ther 20: 525-533.
11. Mehlhorn AT, Schmal H, Kaiser S, Lepski G, Finkenzeller G, Stark GB, Sudkamp NP,. 2006. Mesenchymal stem cells maintain TGF-beta-mediated chondrogenic phenotype in alginate bead culture. Tissue Eng 12: 1393-1403.
12. Mochizuki T, Muneta T, Sakaguchi Y, Nimura A, Yokoyama A, Koga H, Sekiya I,. 2006. Higher chondrogenic potential of fibrous synovium and adipose synovium-derived cells compared with subcutaneous fat-derived cells: Distinguishing properties of mesenchymal stem cells in humans. Arthritis Rheum 54: 843-853.
13. Park JS, Yang HN, Woo DG, Jeon SY, Park KH,. 2012. SOX9 gene plus heparinized TGF-beta 3 coated dexamethasone loaded PLGA microspheres for inducement of chondrogenesis of hMSCs. Biomaterials 33: 7151-7163.
14. Robinson D, Hasharoni A, Cohen N, Yayon A, Moskowitz RM, Nevo Z,. 1999. Fibroblast growth factor receptor-3 as a marker for precartilaginous stem cells. Clin Orthop Relat Res 367 (suppl): 167-175.
15. Suhorutsenko J, Oskolkov N, Arukuusk P, Kurrikoff K, Eriste E, Copolovici DM, Langel U,. 2011. Cell-penetrating peptides, PepFects, show no evidence of toxicity and immunogenicity in vitro and in vivo. Bioconjug Chem 22: 2255-2262.
16. Tang QO, Shakib K, Heliotis M, Tsiridis E, Mantalaris A, Ripamonti U,. 2009. TGF-beta3: A potential biological therapy for enhancing chondrogenesis. Expert Opin Biol Ther 9: 689-701.
17. van den Berg A, Dowdy SF,. 2011. Protein transduction domain delivery of therapeutic macromolecules. Curr Opin Biotechnol 22: 888-893.
18. Wang HY, Chen JX, Sun YX, Deng JZ, Li C, Zhang XZ, Zhuo RX,. 2011. Construction of cell penetrating peptide vectors with N-terminal stearylated nuclear localization signal for targeted delivery of DNA into the cell nuclei. J Control Release 155: 26-33.
19. Yang S, Coles DJ, Esposito A, Mitchell DJ, Toth I, Minchin RF,. 2009. Cellular uptake of self-assembled cationic peptide-DNA complexes: Multifunctional role of the enhancer chloroquine. J Control Release 135: 159-165.
20. You H, Chen A, Liu T, Wang M, Zhang G,. 2011. Construction of eukaryotic expression plasmid of hTGF-beta3 and its inducing effect on differentiation of precartilaginous stem cells into chondroblasts. J Huazhong Univ Sci Technolog Med Sci 31: 524-529.
21. You HB, Chen AM, Cheng H,. 2004. Immunomagnetic separation to isolate and purify precartilaginous stem cells from neonate rats. J Chin Traum (Chinese) 20: 606-608.
22. Zhang S, Chen A, Hu W, Li M, Liao H, Zhu W, Song D, Guo F,. 2008. Immunological purification of rat precartilaginous stem cells and construction of the immortalized cell strain. Arch Orthop Trauma Surg 128: 1339-1344.