A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts

Soil Biology and Biochemistry

Volumn 67, Issue , 2013, Pages 166-173

  Redmile Gordon, M A ^a   Armenise, E ^a   White, R P ^a   Hirsch, P R ^a   Goulding, K W T ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

BRSP; Colorimetric protein assay; EPS; Extracellular polymeric substances; Glomalin related soil protein; GRSP; Humic acid; Polyphenol interference; Soil microbial biofilm; Total protein determination

Indexed keywords

BRSP; EPS; EXTRA-CELLULAR POLYMERIC SUBSTANCES; GLOMALIN-RELATED SOIL PROTEIN; GRSP; HUMIC ACID; POLYPHENOLS; PROTEIN ASSAYS; SOIL MICROBIAL; TOTAL PROTEIN;

ASSAYS; BIOFILMS; COLOR; COLORIMETRY; SOILS;

PROTEINS;

BIOASSAY; BIOFILM; CONCENTRATION (COMPOSITION); ESTIMATION METHOD; EXTRACTION; HUMIC ACID; HUMIC SUBSTANCE; PHENOL; PHENOLIC COMPOUND; PROTEIN; QUANTITATIVE ANALYSIS;

EID: 84884216894     PISSN: 00380717     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.soilbio.2013.08.017     Document Type: Article

References (45)

1. Bianchi D., Golini P., Bortolo R., Cesti P. Immobilization of penicillin G acylase on aminoalkylated polyacrylic supports. Enzym. Microb. Technol. 1996, 18:592-596.
2. Bonmati M., Ceccanti B., Nannipieri P., Valero J. Characterization of humus-protease complexes extracted from soil. Soil Biol. Biochem. 2009, 41:1199-1209.
3. Bradford M.M. Rapid and sensitive method for quantitation of microgram quantities of protein utilising principle of protein dye binding. Analyt. Biochem. 1976, 72:248-254.
4. Burns R.G., DeForest J.L., Marxsen J., Sinsabaugh R.L., Stromberger M.E., Wallenstein M.D., Weintraub M.N., Zoppini A. Soil enzymes in a changing environment: current knowledge and future directions. Soil Biol. Biochem. 2013, 58:216-234.
5. Cesco S., Mimmo T., Tonon G., Tomasi N., Pinton R., Terzano R., Neumann G., Weisskopf L., Renella G., Landi L., Nannipieri P. Plant-borne flavonoids released into the rhizosphere: impact on soil bio-activities related to plant nutrition. A review. Biol. Fertil. Soils 2012, 48:123-149.
6. Chenu C. Clay polysaccharide or sand polysaccharide associations as models for the interface between microorganisms and soil - water related properties and microstructure. Geoderma 1993, 56:143-156.
7. Criquet S., Farnet A.M., Ferre E. Protein measurement in forest litter. Biol. Fertil. Soils 2002, 35:307-313.
8. Emran M., Gispert M., Pardini G. Patterns of soil organic carbon, glomalin and structural stability in abandoned Mediterranean terraced lands. Eur. J. Soil Sci. 2012, 63:637-649.
9. Fernandez I., Carrasco B., Cabaneiro A. Evolution of soil organic matter composition and edaphic carbon effluxes following oak forest clearing for pasture: climate change implications. Eur. J. For. Res. 2012, 131:1681-1693.
10. Ferri F., Bertin L., Scoma A., Marchetti L., Fava F. Recovery of low molecular weight phenols through solid-phase extraction. Chem. Eng. J. 2011, 166:994-1001.
11. Flemming H.C., Wingender J. The biofilm matrix. Nat. Rev. Microbiol. 2010, 8:623-633.
12. Frolund B., Griebe T., Nielsen P.H. Enzymatic-activity in the activated-sludge floc matrix. Appl. Microbiol. Biotechnol. 1995, 43:755-761.
13. Giagnoni L., Magherini F., Landi L., Taghavi S., Modesti A., Bini L., Nannipieri P., Van der Lelie D., Renella G. Extraction of microbial proteome from soil: potential and limitations assessed through a model study. Eur. J. Soil Sci. 2011, 62:74-81.
14. Gillespie A.W., Farrell R.E., Walley F.L., Ross A.R.S., Leinweber P., Eckhardt K.-U., Regier T.Z., Blyth R.I.R. Glomalin-related soil protein contains non-mycorrhizal-related heat-stable proteins, lipids and humic materials. Soil Biol. Biochem. 2011, 43:766-777.
15. Haider K., Martin J.P., Filip Z. Humus biochemistry. Soil Biology and Biochemistry 1975, vol. 4:195-244.
16. Halvorson J.J., Gonzalez J.M. Bradford reactive soil protein in Appalachian soils: distribution and response to incubation, extraction reagent and tannins. Plant Soil 2006, 286:339-356.
17. Janos D.P., Garamszegi S., Beltran B. Glomalin extraction and measurement. Soil Biol. Biochem. 2008, 40:728-739.
18. Ji T.H. Interference by detergents, chelating agents, and buffers with Lowry protein determination. Analyt. Biochem. 1973, 52:517-521.
19. Kuzyakov Y., Friedel J.K., Stahr K. Review of mechanisms and quantification of priming effects. Soil Biol. Biochem. 2000, 32:1485-1498.
20. Lipinska H., Wanda H. Allelopathic effects of Poa pratensis on other grassland spp. Allelopath. J. 2005, 16:251-259.
21. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin Phenol reagent. J.Biol. Chem. 1951, 193:265-275.
22. Lucarini A.C., Kilikian B.V. Comparative study of Lowry and Bradford methods: interfering substances. Biotechnol. Tech. 1999, 13:149-154.
23. Martens D.A., Reedy T.E., Lewis D.T. Soil organic carbon content and composition of 130-year crop, pasture and forest land-use managements. Global Change Biol. 2004, 10:65-78.
24. Miller G.L. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analyt. Biochem. 1959, 48:422-427.
25. Nannipieri P., Eldor P. The chemical and functional characterization of soil N and its biotic components. Soil Biol. Biochem. 2009, 41:2357-2369.
26. Oosta G.M., Mathewson N.S., Catravas G.N. Optimisation of Foilin-Ciocalteu reagent concentration in an automated Lowry protein assay. Analyt. Biochem. 1978, 89:31-34.
27. Or D., Smets B.F., Wraith J.M., Dechesne A., Friedman S.P. Physical constraints affecting bacterial habitats and activity in unsaturated porous media - a review. Adv. Water Resour. 2007, 30:1505-1527.
28. Peterson G.L. Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall. Analyt. Biochem. 1979, 100:201-220.
29. Rillig M.C., Mardatin N.F., Leifheit E.F., Antunes P.M. Mycelium of arbuscular mycorrhizal fungi increases soil water repellency and is sufficient to maintain water-stable soil aggregates. Soil Biol. Biochem. 2010, 42:1189-1191.
30. Roberson E.B., Firestone M.K. Relationship between desiccation and exopolysaccharide production in a soil pseudomonas sp. Appl. Environ. Microbiol. 1992, 58:1284-1291.
31. Roberts P., Jones D.L. Critical evaluation of methods for determining total protein in soil solution. Soil Biol. Biochem. 2008, 40:1485-1495.
32. Rosier C.L., Hoye A.T., Rillig M.C. Glomalin-related soil protein: assessment of current detection and quantification tools. Soil Biol. Biochem. 2006, 38:2205-2211.
33. Sapan C.V., Lundblad R.L., Price N.C. Colorimetric protein assay techniques. Biotechnol. Appl. Biochem. 1999, 29:99-108.
34. Schindler F.V., Mercer E.J., Rice J.A. Chemical characteristics of glomalin-related soil protein (GRSP) extracted from soils of varying organic matter content. Soil Biol. Biochem. 2007, 39:320-329.
35. Schmidt M.W.I., Torn M.S., Abiven S., Dittmar T., Guggenberger G., Janssens I.A., Kleber M., Koegel-Knabner I., Lehmann J., Manning D.A.C., Nannipieri P., Rasse D.P., Weiner S., Trumbore S.E. Persistence of soil organic matter as an ecosystem property. Nature 2011, 478:49-56.
36. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid. Analyt. Biochem. 1985, 150:76-85.
37. Tang J., Mo Y., Zhang J., Zhang R. Influence of biological aggregating agents associated with microbial population on soil aggregate stability. Appl. Soil Ecol. 2011, 47:153-159.
38. Taylor E.B., Williams M.A. Microbial protein in soil: influence of extraction method and C amendment on extraction and recovery. Microb. Ecol. 2010, 59:390-399.
39. Wallach D., Goffinet B. Mean squared error of prediction in models for studying ecological and agronomic systems. Biometrics 1987, 43:561-573.
40. Watts C.W., Whalley W.R., Brookes P.C., Devonshire B.J., Whitmore A.P. Biological and physical processes that mediate micro-aggregation of clays. Soil Sci. 2005, 170:573-583.
41. Whiffen L.K., Midgley D.J., McGee P.A. Polyphenolic compounds interfere with quantification of protein in soil extracts using the Bradford method. Soil Biol. Biochem. 2007, 39:691-694.
42. Wright S.F., Upadhyaya A. Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Sci. 1996, 161:575-586.
43. Wright S.F., Upadhyaya A. Asurvey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant Soil 1998, 198:97-107.
44. Wu L.Q., Yang B., Bi J.M., Wang J. Development of bovine serum albumin certified reference. Analyt. Bioanal. Chem. 2011, 400:3443-3449.
45. Young I.M., Feeney D.S., O'Donnell A.G., Goulding K.W.T. Fungi in century old managed soils could hold key to the development of soil water repellency. Soil Biol. Biochem. 2012, 45:125-127.