Detection of antigen-specific T cells based on intracellular cytokine staining using flow-cytometry

Methods in Molecular Biology

Volumn 1064, Issue , 2013, Pages 267-274

  Schmidt, Tina ^a   Sester, Martina ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Antigen; Cytokines; Flow cytometry; Immune response; Interferon ; T cell

Indexed keywords

BREFELDIN A; CYTOKINE;

ANTIBODY LABELING; ANTIGEN DETECTION; ANTIGEN SPECIFICITY; ARTICLE; CD4+ T LYMPHOCYTE; CELL LYSATE; CELL STIMULATION; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOMEGALOVIRUS; FLOW CYTOMETRY; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; STAINING;

ANTIGENS; CYTOKINES; FLOW CYTOMETRY; HUMANS; INTRACELLULAR SPACE; STAINING AND LABELING; T-LYMPHOCYTES;

EID: 84884178102     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-601-6_19     Document Type: Article

References (3)

1. Sester M, Sester U, Gärtner B, Heine G, Girndt M, Mueller-Lantzsch N et al (2001) Levels of virus-specific CD4 T cells correlate with cytomegalovirus control and predict virus-induced disease after renal transplantation. Transplantation 71(9):1287-1294
2. Sester M, Gärtner BC, Sester U (2008) Monitoring of CMV specific T-cell levels after organ transplantation. J Lab Med 32(3):121-130
3. Schmidt T, Dirks J, Enders M, Gärtner BC, Uhlmann-Schiffler H, Sester U et al (2012) CD4+ T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal antigens and functionally differs from active influenza infection. Eur J Immunol 42(7): 1755-1766