Stable transformation of Medicago truncatula cv. Jemalong for gene analysis using Agrobacterium tumefaciens

Methods in Molecular Biology

Volumn 1069, Issue , 2013, Pages 203-214

  Song, Youhong ^a   Nolan, Kim E ^a   Rose, Ray J ^b  

^a UNIVERSITY OF NEWCASTLE   (Australia)

^b UNIVERSITY OF NEWCASTLE   (Australia)

Author keywords

Agrobacterium tumefaciens; Binary vectors; Jemalong 2HA; Medicago truncatula; Regeneration; Somatic embryogenesis; Stable transformation; Tissue culture; Transgenes

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BARREL MEDIC; CONTROLLED STUDY; EMBRYO DEVELOPMENT; GENETIC ANALYSIS; GENOTYPE; NONHUMAN; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER; TISSUE CULTURE; TRANSGENE;

AGROBACTERIUM TUMEFACIENS; GENE EXPRESSION PROFILING; GENETIC VECTORS; MEDICAGO TRUNCATULA; PLANTS, GENETICALLY MODIFIED; TRANSFORMATION, BACTERIAL; TRANSFORMATION, GENETIC;

EID: 84884176239     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-613-9_14     Document Type: Article

References (32)

1. Barker DG, Bianchi S, Blondon F, Datteé Y, Duc G, Essad S et al (1990) Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis. Plant Mol Biol Rep 8:40-49
2. Cook DR (1999) Medicago truncatula-a model in the making! Curr Opin Plant Biol 2:301-304
3. Rose RJ (2008) Medicago truncatula as a model for understanding plant interactions with other organisms, plant development and stress biology: past, present and future. Funct Plant Biol 35:253-264
4. Young ND, Debellé F, Oldroyd GED, Geurts R, Cannon SB, Udvardi MK et al (2011) The Medicago genome provides insight into the evolution of rhizobial symbioses. Nature 480:520-524
5. Tesfaye M, Silverstein KAT, Bucciarelli B, Samac DA, Vance CP (2006) The Affymetrix Medicago GeneChip ® array is applicable for transcript analysis of alfalfa (Medicago sativa). Funct Plant Biol 33:783-788
6. Benedito VA, Torres-Jerez I, Murray JD, Andriankaja A, Allen S, Kakar K et al (2008) A gene expression atlas of the model legume Medicago truncatula. Plant J 55:504-513
7. Cheng X, Wen J, Tadege M, Ratet P, Mysore KS (2011) Reverse genetics in Medicago truncatula using Tnt1 insertion mutants. In Plant reverse genetics: methods and protocols (ed. A. Pereira). Methods Mol Biol 678:179-190
8. Watson BS, Asirvatham VS, Wang L, Sumner LW (2003) Mapping the proteome of barrel medic (Medicago truncatula). Plant Physiol 131:1104-1123
9. Gallardo K, Le Signor C, Vandekerckhove J, Thompson RD, Burstin J (2003) Proteomics of Medicago truncatula seed development establishes the time frame of diverse metabolic processes related to reserve accumulation. Plant Physiol 133:664-682
10. Imin N, de Jong F, Mathesius U, van Noorden G, Saeed NA, Wang X-D, Rose RJ, Rolfe BG (2004) Proteome reference maps of Medicago truncatula embryogenic cell cultures generated from single protoplasts. Proteomics 4: 1883-1896
11. Imin N, Nizamidin M, Daniher D, Nolan KE, Rose RJ, Rolfe BG (2005) Proteomic analysis of somatic embryogenesis in Medicago truncatula. Explant cultures grown under 6-benzylaminopurine and 1-naphthaleneacetic acid treatments. Plant Physiol 137:1250-1260
12. Szittya G, Moxon S, Santos DM, Jing R, Fevereiro MPS, Moulton V, Dalmay T (2008) High-throughput sequencing of Medicago truncatula short RNAs identifies eight new miRNA families. BMC Genomics 9:593
13. Lelandais-Briére C, Naya L, Sallet E, Calenge F, Frugier F, Hartmann C, Gouzy J, Crespi M (2009) Genome-wide Medicago truncatula small RNA analysis revealed novel microRNAs and isoforms differentially regulated in roots and nodules. Plant Cell 21:2780-2796
14. Chen L, Wang T, Zhao M, Zhang W (2012) Ethylene-responsive miRNAs in roots of Medicago truncatula identified by highthroughput sequencing at whole genome level. Plant Sci 184:14-19
15. Mantiri FR, Kurdyukov S, Lohar DP, Sharapova N, Saeed NA, VandenBosch KA, Rose RJ (2008) The transcription factor MtSERF1 of the ERF subfamily identified by transcriptional profiling is required for somatic embryogenesis induced by auxin plus cytokinin in Medicago truncatula. Plant Physiol 146:1622-1636
16. Jayasena KW, Hajimorad MR, Law EG, Rehman A-U, Nolan KE, Zanker T, Rose RJ, Randles JW (2001) Resistance to Alfalfa mosaic virus in transgenic barrel medic lines containing the virus coat protein gene. Aust J Agric Res 52:67-72
17. Nolan KE, Kurdyukov S, Rose RJ (2009) Expression of the SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1 (SERK1) gene is associated with developmental change in the life cycle of the model legume Medicago truncatula. J Exp Bot 60:1759-1771
18. Nolan KE, Rose RJ, Gorst JR (1989) Regeneration of Medicago truncatula from tissue culture: increased somatic embryogenesis using explants from regenerated plants. Plant Cell Rep 8:278-281
19. Rose RJ, Nolan KE, Bicego L (1999) The development of the highly regenerable seed line Jemalong 2HA for transformation of Medicago truncatula-implications for regenerability via somatic embryogenesis. J Plant Physiol 155:788-791
20. Hoffmann B, Trinh TH, Leung J, Kondorosi A, Kondorosi E (1997) A new Medicago truncatula line with superior in vitro regeneration, transformation, and symbiotic properties isolated through cell culture selection. Mol Plant-Microbe Interact 10:307-315
21. Thomas MR, Rose RJ, Nolan KE (1992) Genetic transformation of Medicago truncatula using Agrobacterium with genetically modified Ri and disarmed Ti plasmids. Plant Cell Rep 11:113-117
22. Wang JH, Rose RJ, Donaldson BI (1996) Agrobacterium-mediated transformation and expression of foreign genes in Medicago truncatula. Aust J Plant Physiol 23:265-270
23. Chabaud M, Larsonneau C, Marmouget C, Huguet T (1996) Transformation of barrel medic (Medicago truncatula Gaertn.) by Agrobacterium tumefaciens and regeneration via somatic embryogenesis of transgenic plants with the MtENOD12 nodulin promoter fused to the gus reporter gene. Plant Cell Rep 15: 305-310
24. Thomas MR, Johnson LB, White FF (1990) Selection of interspecific somatic hybrids of Medicago by using Agrobacterium transformed tissues. Plant Sci 69:189-198
25. Nolan KE, Rose RJ (1998) Plant regeneration from cultured Medicago truncatula with particular reference to abscisic acid and light treatments. Aust J Bot 46:151-160
26. Rose RJ, Nolan KE (1995) Regeneration of Medicago truncatula from protoplasts isolated from kanamycin-sensitive and kanamycinresistant plants. Plant Cell Rep 14:349-353
27. Chabaud M, de Carvalho-Niebel F, Barker DG (2003) Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1. Plant Cell Rep 22:46-51
28. Lazo GR, Stein PA, Ludwig RA (1991) A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Nat Biotechnol 9:963-967
29. Hellens R, Mullineaux P, Klee H (2000) A guide to Agrobacterium binary Ti vectors. Trends Plant Sci 5:446-452
30. Curtis MD, Grossniklaus U (2003) A gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol 133: 462-469
31. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497
32. Dalton CC, Iqbal K, Turner DA (1983) Iron phosphate precipitation in Murasgige and Skoog media. Physiol Plant 57:472-476