Extraction, purification and nanoformulation of natural phycocyanin (from Klamath algae) for dermal and deeper soft tissue delivery

Journal of Biomedical Nanotechnology

Volumn 9, Issue 11, 2013, Pages 1929-1938

  Caddeo, Carla ^a   Chessa, Maura ^a   Vassallo, Antonio ^b   Pons, Ramon ^c   Diez Sales, Octavio ^d   Fadda, Anna Maria ^a   Manconi, Maria ^a  

^a UNIVERSITY OF CAGLIARI   (Italy)

^b UNIVERSITY OF BASILICATA   (Italy)

^c INSTITUTE OF ADVANCED CHEMISTRY OF CATALONIA IQAC CSIC   (Spain)

^d UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Human skin; Phospholipid vesicle; Phycocyanin; Protein extraction and purification; Topical delivery

Indexed keywords

HUMAN SKIN; PHOSPHOLIPID VESICLES; PHYCOCYANIN; PROTEIN EXTRACTION; TOPICAL DELIVERY;

ALGAE; CATALYST ACTIVITY; ELECTROPHORESIS; LIPOSOMES; POLYOLS; PROTEINS; PURIFICATION; SCANNING ELECTRON MICROSCOPY; SKIN;

EXTRACTION;

ANTIINFLAMMATORY AGENT; LIPOSOME; PENETRATION ENHANCING AGENT; PHOSPHOLIPID; PHYCOCYANIN; PROPYLENE GLYCOL;

ALGA; ARTICLE; DRUG FORMULATION; DRUG ISOLATION; DRUG PURIFICATION; KLAMATH; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NANOFORMULATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOFT TISSUE;

ABSORPTION; ADMINISTRATION, TOPICAL; CYANOBACTERIA; DRUG COMPOUNDING; HUMANS; LIPOSOMES; NANOCAPSULES; PARTICLE SIZE; PHYCOCYANIN; SKIN; TISSUE DISTRIBUTION;

EID: 84883675800     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2013.1741     Document Type: Article

References (37)

1. M. Brunner, P. Dehghanyar, B. Seigfried, W. Martin, G. Menke, and M. Müller, Favourable dermal penetration of diclofenac after administration to the skin using a novel spray gel formulation. Br. J. Clin. Pharmacol. 60, 573 (2005).
2. I. Tegeder, U. Muth-Selbach, J. Lotsch, G. Rusing, R. Oelkers, K. Brune, S. Meller, G. R. Kelm, F. Sorgel, and G. Geisslinger, Application of microdialysis for the determination of muscle and subcutaneous tissue concentrations after oral and topical ibuprofen administration. Clin. Pharmacol. Ther. 65, 357 (1999).
3. D. Paolino, G. Lucania, D. Mardente, F. Alhaique, and M. Fresta, Ethosomes for skin delivery of ammonium glycyrrhizinate: In vitro percutaneous permeation through human skin and in vivo antiinflammatory activity on human volunteers. J. Control. Release 106, 99 (2005).
4. G. Nava, E. Piñón, L. Mendoza, N. Mendoza, D. Quintanar, and A. Ganem, Formulation and in Vitro, ex Vivo and in Vivo evaluation of elastic liposomes for transdermal delivery of Ketorolac Tromethamine. Pharmaceutics 3, 954 (2011).
5. L. Tavano, R. Muzzalupo, S. Trombino, R. Cassano, A. Pingitore, and N. Picci, Effect of formulations variables on the in vitro percutaneous permeation of sodium diclofenac from new vesicular systems obtained from pluronic triblock copolymers. Colloid Surf. BBiointerfaces 79, 227 (2010).
6. D. Paolino, R. Muzzalupo, A. Ricciardi, C. Celia, N. Picci, and M. Fresta, In vitro and in vivo evaluation of Bola-surfactant containing niosomes for transdermal delivery. Biomed. Microdevices 9, 421 (2007).
7. S. Mura, F. Pirot, M. Manconi, F. Falson, and A. M. Fadda, Liposomes and niosomes as potential cariers for dermal delivery of minoxidil. J. Drug Target. 15, 101 (2007).
8. C. Celia, F. Cilurzo, E. Trapasso, D. Cosco, M. Fresta, and D. Paolino, Ethosomes® and transfersomes® containing linoleic acid: Physicochemical and technological features of topical drug delivery carriers for the potential treatment of melasma disorders. Biomed. Microdevices 14, 119 (2012).
9. M. M. A. Elsayed, O. Y. Abdallah, V. F. Naggar, and N. M. Khalafallah, PG-liposomes: Novel lipid vesicles for skin delivery of drugs. J. Pharm. Pharmacol. 59, 1447 (2007).
10. N. Dragicevic-Curic, D. Scheglmann, V. Albrecht, and A. Fahr, Development of different temoporfin-loaded invasomes-novel nanocarriers of temoporfin: Characterization, stability and in vitro skin penetration studies. Colloid Surf. B-Biointerfaces 70, 198 (2009).
11. M. Manconi, S. Mura, C. Sinico, A. M. Fadda, A. Vila, and F. Molina, Development and characterization of liposomes containing glycols as carriers for diclofenac. Colloid Surf. A-Physicochem. Eng. Asp 342, 53 (2009).
12. M. Manconi, C. Caddeo, C. Sinico, D. Valenti, M. C. Mostallino, G. Biggio, and A. M. Fadda, Ex vivo skin delivery of diclofenac by transcutol containing liposomes and suggested mechanism of vesicleskin interaction. Eur. J. Pharm. Biopharm. 78, 27 (2011).
13. M. Manconi, C. Sinico, C. Caddeo, A. O. Vila, D. Valenti, and A. M. Fadda, Penetration enhancer containing vesicles as carriers for dermal delivery of tretinoin. Int. J. Pharm. 412, 37 (2011).
14. S. Mura, M. Manconi, C. Sinico, D. Valenti, and A. M. Fadda, Penetration enhancer containing vesicles (PEVs) as carriers for cutaneous delivery of minoxidil. Int. J. Pharm. 380, 72 (2009).
15. S. Mura, M. Manconi, D. Valenti, C. Sinico, A. O. Vila, and A. M. Fadda, Transcutol containing vesicles for topical delivery of minoxidil. J. Drug Target. 19, 189 (2011).
16. M. Manconi, C. Caddeo, C. Sinico, D. Valenti, M. C. Mostallino, S. Lampis, M. Monduzzi, and A. M. Fadda, Penetration enhancercontaining vesicles: Composition dependence of structural features and skin penetration ability. Eur. J. Pharm. Biopharm. 82, 352 (2012).
17. R. M. Elmoslemany, O. Y. Abdallah, L. K. El-Khordagui, and N. M. Khalafallah, Propylene Glycol Liposomes as a topical delivery system for Miconazole Nitrate: Comparison with conventional Liposomes. AAPS Pharm. Sci. Tech. 13, 723 (2012).
18. M. Manconi, S. Mura, M. Manca, A. M. Fadda, M. Dolz, M. Hernandez, A. Casanovas, and O. Diez-Sales, Int. J. Pharm. 392, 92 (2010).
19. N. T. Eriksen, Production of phycocyanin a pigment with applications in biology, biotechnology, foods and medicine. Appl. Microbiol. Biotechnol. 80, 1 (2008).
20. C. Romay, J. Armesto, D. Remirez, R. Gonzalez, N. Ledon, and I. Garcia, Antioxidant and anti-inflammatory properties of cphycocyanin from blue-green algae. Inflamm. Res. 47, 36 (1998).
21. C. Reddy, V. B. Bhat, G. Kiranmai, M. Reddy, P. Reddanna, and K. Madyastha, Selective inhibition of cyclooxygenase-2 by cphycocyanin, a biliprotein from spirulina platensis. Biochem. Bioph. Res. Co. 277, 599 (2000).
22. M. Manconi, J. Pendás, N. Ledón, T. Moreira, C. Sinico, L. Saso, and A. M. Fadda, Phycocyanin liposomes for topical anti-inflammatory activity: In-vitro in-vivo studies. J. Pharm. Pharmacol. 61, 423 (2009).
23. H. P. Singh, P. Utreja, A. K. Tiwary, and S. Jain, Elastic liposomal formulation for sustained delivery of colchicine: In vitro characterization and in vivo evaluation of anti-gout activity. AAPS J. 11, 54 (2009).
24. S. Benedetti, S. Rinalducci, F. Benvenuti, S. Francogli, S. Pagliarani, L. Giorgi, M. Micheloni, G. M. D'Amici, L. Zolla, and F. Canestrari, Purification and characterization of phycocyanin from the blue-green algae Aphanizomenon flos-aquae. J. Chromatogr. B 883, 12 (2006).
25. A. Shevchenko, M. Wilm, O. Vorm, and M. Mann, Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal. Chem. 68, 850 (1996).
26. C. Caddeo, K. Teskač, C. Sinico, and J. Kristl, Effect of resveratrol incorporated in liposomes on proliferation and UV-B protection of cells. Int. J. Pharm. 363, 183 (2008).
27. M. Chessa, C. Caddeo, D. Valenti, M. Manconi, C. Sinico, and A. M. Fadda, Effect of Penetration Enhancer containing Vesicles on the percutaneous delivery of quercetin through new born pig skin. Pharmaceutics 3, 497 (2011).
28. G. Pabst, M. Rappolt, H. Amenitsch, and P. Laggner, Structural information from multilamellar liposomes at full hydration: Full qrange fitting with high quality X-ray data. Phys. Rev. E Stat. Phys. Plasmas Fluids Relat. Interdiscip. Top. 62, 4000 (2000).
29. H. Madhyastha, K. Radha, M. Sugiki, S. Omura, and M. Maruyama, Purification of c-phycocyanin from spirulina fusiformis and its effect on the induction of urokinase-type plasminogen activator from calf pulmonary endothelial cells. Phytomedicine 13, 564 (2006).
30. S. Rinalducci, P. Roepstorff, and L. Zolla, De novo sequence analysis and intact mass measurements for characterization of phycocyanin subunit isoforms from the blue-green alga Aphanizomenon flos-aquae. J. Mass. Spectrom. 44, 503 (2009).
31. T. Chen, Y.-S. Wong, and W. Zheng, Purification and characterization of selenium-containing phycocyanin from selenium-enriched Spirulina platensis. Phytochemistry 67, 2424 (2006).
32. S.-G. Yan, L.-P. Zhu, H.-N. Su, X.-Y. Zhang, X.-L. Chen, B.-C. Zhou, and Y.-Z. Zhang, Single-step chromatography for simultaneous purification of C-phycocyanin and allophycocyanin with high purity and recovery from Spirulina (Arthrospira) platensis. J. Appl. Phycol. 23, 1 (2011).
33. M. Manconi, R. Isola, A. M. Falchi, C. Sinico, and A. M. Fadda, Intracellular distribution of fluorescent probes delivered by vesicles of different lipidic composition. Colloid Surf. B-Biointerfaces 57, 143 (2007).
34. X. Xu, M. A. Khan, and D. J. Burgess, Predicting hydrophilic drug encapsulation in unilamellar liposomes. Int. J. Pharm. 423, 410 (2012).
35. E. Touitou, N. Dayan, L. Bergelson, B. Godin, and M. Eliaz, Ethosomes novel vesicular carriers for enhanced delivery: Characterization and skin penetration properties. J. Control. Release 65, 403 (2000).
36. D. Ainbinder, D. Paolino, M. Fresta, and E. Touitou, Drug delivery applications with Ethosomes. J. Biomed. Nanotechnol. 6, 558 (2010).
37. G. Tan, P. Xu, L. B. Lawson, J. He, L. C. Freytag, J. D. Clements, and V. T. John, Hydration effects on skin microstructure as probed by high-resolution cryo-scanning electron microscopy and mechanistic implications to enhanced transcutaneous delivery of biomacromolecules. J. Pharm. Sci. 99, 730 (2010).