Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform

Methods in Enzymology

Volumn 529, Issue , 2013, Pages 185-200

  Yegnasubramanian, Srinivasan ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL DNA; BUFFER; DEOXYADENOSINE TRIPHOSPHATE; DEOXYCYTIDINE TRIPHOSPHATE; DEOXYGUANOSINE TRIPHOSPHATE; DITHIOTHREITOL; DNA FRAGMENT; DNA POLYMERASE; DOUBLE STRANDED DNA; EDETIC ACID; GENOMIC DNA; MACROGOL; MAGNESIUM CHLORIDE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; POLYNUCLEOTIDE 5' HYDROXYL KINASE; THYMIDINE TRIPHOSPHATE;

AGAR GEL ELECTROPHORESIS; ARTICLE; BIOSAFETY; CENTRIFUGATION; DENATURATION; DNA ISOLATION; DNA LIBRARY; DNA NICK TRANSLATION; DNA SEQUENCE; DNA TEMPLATE; ENZYME PHOSPHORYLATION; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; SPECTROPHOTOMETER; ULTRASOUND; WATER QUALITY; WORKFLOW;

ADAPTOR LIGATION; AMPLIFICATION; DNA FRAGMENTS; END-REPAIR OF FRAGMENTED DNA; INTEGRATED DNA TECHNOLOGIES (IDT); INVITROGEN E-GEL SYSTEM; NEXT-GENERATION SEQUENCING;

DNA; GENE LIBRARY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; POLYMERASE CHAIN REACTION;

EID: 84883596779     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-418687-3.00015-X     Document Type: Chapter

References (2)

1. M.L. Metzker Sequencing technologies - The next generation Nature Reviews Genetics 11 2010 31 46
2. These protocols were adapted from the Applied Biosystems protocols in the Applied Biosystems SOLiD™ 4 System Library Preparation Guide (www.appliedbiosystems.com), as well as the protocols supplied with the NEBNext DNA Sample Prep Master Mix Set 3 (NEB E6060S/L) kit.