Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines

Methods in Enzymology

Volumn 529, Issue , 2013, Pages 99-124

  Spitzer, Jessica ^a   Landthaler, Markus ^b   Tuschl, Thomas ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; MALTOSE BINDING PROTEIN; MUTANT PROTEIN; NUCLEIC ACID; RECOMBINANT PROTEIN; RNA BINDING PROTEIN;

ANTIBODY AFFINITY; ARTICLE; CELL CLONING; CELL LINE; CULTURE MEDIUM; EXPRESSION VECTOR; GENETIC RECOMBINATION; MAMMAL CELL; MOLECULAR CLONING; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STANDARDIZATION;

MAMMALIA;

FLP-IN T-REX® LINES; GATEWAY EXPRESSION VECTORS; GATEWAY® RECOMBINATION CLONING TECHNOLOGY; MOLECULAR CLONING; STABLE CELL LINES;

ANIMALS; CELL LINE; CLONING, MOLECULAR; GENE EXPRESSION REGULATION; GENETIC VECTORS; HUMANS; MAMMALS; PROTEINS; RECOMBINATION, GENETIC; RNA-BINDING PROTEINS;

EID: 84883593530     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-418687-3.00008-2     Document Type: Chapter

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