Synthesis of [18F]-Fluorodeoxyglucose ([18F]FDG)

Radiochemical Syntheses: Radiopharmaceuticals for Positron Emission Tomography

Volumn 1, Issue , 2012, Pages 1-13

  Richards, Michelle L ^a   Scott, Peter J H ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Fluorine 18 labeled radiopharmaceuticals; Radiochemical identity and purity; Synthesis of 18F fluorodeoxyglucose ( 18F FDG)

Indexed keywords

EID: 84883558281     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781118140345.ch1     Document Type: Chapter

References (56)

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19. GE Healthcare, USA.
20. TRACERlab MXFDG Cassettes were purchased from General Electric Healthcare (part no. P5150ME), and used as received. The cassettes came prepackaged and assembled with two tC18 cartridges and one alumina-N cartridge.
21. Millex-FG sterile 0.20 μm filters were purchased from Millipore (part no. SLFG025LS) and used as received.
22. Conical Column 10mL Reservoir was purchased from ABX (part no. 9180) and used as received.
23. QMA-Light Sep-Pak® preconditioned cartridges (part no. K-920) were purchased from Advanced Biochemical Compounds (ABX) and used as received.
24. 30mL syringes were purchased from Fischer Scientific (part no. 309650) and used as received.
25. Mannose triflate ultrapure precursor was purchased from ABX (part no. 101.0040) and used as received.
26. Coincidence FDG18 Synthesizer Reagent Kit was purchased from ABX (part no. K-153TM) and used as received.
27. GEMS silver high-yield [18F]fluoride target.
28. 18O]H2O was purchased from ABX, Amic, Rotem, or Medical Isotopes, and used as received.
29. Millex-GS sterile 0.22-μm filters were purchased from Millipore (part no. SLGSV255F) and used as received.
30. Sterile 30-mL dose vial was purchased from Hospira (part no. 5829-30), and used as received.
31. U.S. Pharmacopeia <823> Radiopharmaceuticals for positron emission tomography compounding. USP 32-NF 27; 2009.
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33. Whatman Thin Layer Chromatography Plates (part no. 4861-110) MK6F Silica Gel 60 A° , Dimensions 2.5 × 7.5 cm, layer thickness 250 μm.
34. Bioscan, Inc., USA.
35. The FDG reference standard solution contained both 2-fluoro-2-deoxy-d-glucose reference standard, purchased from Sigma Aldrich (part no. F5006) and used as received, and anhydrous acetonitrile, purchased from Acros (part no. 61096-1000) and used as received.
36. The TLC developing solution contained anhydrous acetonitrile, purchased from Acros (part no. 61096-1000) and used as received and MilliQ water, in concentrations of 95% and 5%, respectively.
37. Laboratory Hot Plate, manufactured by Corning, Model no. PC-200, and used at temperature setting 5.
38. Capintec, Inc., USA.
39. Shimadzu Corporation, USA.
40. GC Column: Restek Stabliwax with Integra-Guard; 30 m × 0.25 mm, purchased from Restek (part no. 10623-124).
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44. Kryptofix-[2.2.2] was purchased from Acros (part no. 29195-0010) and used as received for preparation of reference standard.
45. Chloroplatinic acid was purchased from Acros (part no. 363590010) and used as received.
46. Potassium iodide was purchased from Acros (part no. 19379-0500) and used as received.
47. Thin-layer silica gel sheets were purchased from EMD Chemicals (part no. 57487), 20 × 20 cm sheets, silica gel 60 A° , and cut into squares 4 × 5 cm.
48. Charles River Laboratories, USA.
49. Endosafe Portable Testing System (model no. PTS 100), purchased and calibrated according to manufacture guidelines by Charles River Laboratories, USA.
50. Limulus Amebocyte Lysate (LAL) Test Cartridges (part no. PTS20F) purchased from Charles River Laboratories and used as received.
51. Endosafe Limulus Amebocyte Lysate (LAL) Reagent water (part no. W110) purchased from Charles River Laboratories and used as received.
52. VWR Vortexer 2 (model no. G-560), vortex speed setting 8.
53. US Pharmacopeia <85>. Bacterial endotoxins test. USP 32-NF 27, 2009.
54. Fluid Thioglycolate Media (part no. 220889) purchased from Beckon, Dickson and Company, USA, and used as received.
55. Soybean Casein Digest Media (part no. 221823) purchased from Beckon, Dickson and Company, USA, and used as received.
56. US Pharmacopeia <71> Sterility tests. USP 32-NF 27, 2009.