Comparative Evaluation of GenoType MTBDRplus Line Probe Assay with Solid Culture Method in Early Diagnosis of Multidrug Resistant Tuberculosis (MDR-TB) at a Tertiary Care Centre in India

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Yadav, Raj N ^a   Singh, Binit K ^a   Sharma, Surendra K ^a   Sharma, Rohini ^a   Soneja, Manish ^a   Sreenivas, Vishnubhatla ^a   Myneedu, Vithal P ^b   Hanif, Mahmud ^c   Kumar, Ashok ^d   Sachdeva, Kuldeep S ^d   Paramasivan, Chinnambedu N ^e   Vollepore, Balasangameshwra ^e   Thakur, Rahul ^e   Raizada, Neeraj ^e   Arora, Suresh K ^f   Sinha, Sanjeev ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b Lala Ram Sarup Institute of Tuberculosis and Respiratory Diseases   (India)

^c New Delhi TB Centre   (India)

^d GOVERNMENT OF INDIA   (India)

^e Foundation for Innovative New Diagnostics   (India)

^f State TB Office   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ISONIAZID; KATG PROTEIN; RIFAMPICIN;

ADULT; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; ASSAY; CONTROLLED STUDY; CROSS-SECTIONAL STUDY; CULTURE TECHNIQUE; DIAGNOSTIC TEST ACCURACY STUDY; EARLY DIAGNOSIS; FEMALE; GENE MUTATION; HUMAN; INDIA; INHA GENE; KATG GENE; LINE PROBE ASSAY; MAJOR CLINICAL STUDY; MALE; MULTIDRUG RESISTANT TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; SENSITIVITY AND SPECIFICITY; SOLID CULTURE METHOD; SPUTUM SMEAR; TERTIARY HEALTH CARE; TURNAROUND TIME;

ADULT; ANTIBIOTICS, ANTITUBERCULAR; BACTERIAL PROTEINS; CATALASE; CROSS-SECTIONAL STUDIES; CULTURE TECHNIQUES; DRUG RESISTANCE, MULTIPLE, BACTERIAL; EARLY DIAGNOSIS; FEMALE; GENOTYPING TECHNIQUES; HUMANS; INDIA; ISONIAZID; MALE; MICROBIAL SENSITIVITY TESTS; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID HYBRIDIZATION; OXIDOREDUCTASES; RIFAMPIN; SENSITIVITY AND SPECIFICITY; SPUTUM; TERTIARY CARE CENTERS; TUBERCULOSIS, MULTIDRUG-RESISTANT; YOUNG ADULT;

EID: 84883514211     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0072036     Document Type: Article

References (28)

1. Sharma SK, Mohan A, (2006) Multidrug-Resistant Tuberculosis: A menace that threatens to destabilize tuberculosis control. Chest 130: 261-272.
2. World Health Organization (2012) Tuberculosis control in south east Asia region. The regional report 2012. SEA/TB/338. Geneva: WHO country office Thailand website. Available: http://whothailand.healthrepository.org/bitstream/123456789/1430/1/2012%20Report-Tuberculosis%20Control%20in%20SE%20Asia%20Region.pdf. Accessed 2013 July 15.
3. World Health Organization (2010) Multidrug and extensively drug-resistant TB (M/XDR-TB): 2010 Global report on surveillance and response. WHO/HTM/TB/2010.3. Geneva (2010). WHO website. Available: http://apps.who.int/iris/bitstream/10665/44286/1/9789241599191_eng.pdf. Accessed 2013 July 15.
4. World Health Organization (2008) Molecular line probe assays for rapid screening of patients at risk of multi-drug resistant tuberculosis (MDR-TB). Policy statement. Genewa (2008). TBC India website. Available: http://www.who.int/tb/features_archive/policy_statement.pdf. Accessed 2013 July 15.
5. Hilleman D, Rusch-Gerdes S, Richter E, (2007) Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens. J Clin Microbiol 45: 2635-2640.
6. Central TB Division, Directorate General of Health Services, Ministry of Health & Family Welfare, Government of India (2005) Revised National Tuberculosis Control Programme Laboratory Network (2005) Guidelines for quality assurance of smear microscopy for diagnosing tuberculosis. TBC India website.Available: http://www.tbcindia.nic.in/pdfs/RNTCP%20Lab%20Network%20Guidelines.pdf. Accessed 2013 July 15.
7. Central TB Division, Directorate General of Health Services, Ministry of Health & Family Welfare, Government of India (2010) Revised National Tuberculosis Programme, DOTS-Plus Guidelines. TBC India website. Available: http://www.tbcindia.nic.in/pdfs/DOTS_Plus_Guidelines_Jan2010.pdf. Accessed 2013 July 15.
8. Buyankhishig B, Oyuntuya T, Tserelmaa B, Sarantuya J, Lucero MG, et al. (2012) Rapid molecular testing for multi-resistant tuberculosis in mongoliya: A diagnostic accuracy study. Int J Mycobacteriol: 40-44.
9. Central TB Division, Directorate General of Health Services, Ministry of Health & Family welfare, Government of India (2009) Revised National Tuberculosis Programme, Culture of Mycobacterium tuberculosis and drug susceptibility testing on solid medium. Manual of standard operating procedures. TBC India website. Available: http://www.tbcindia.nic.in/pdfs/standard%20operating%20procedures%20for%20C&DST%20labs.pdf. Accessed 2013 July 15.
10. Albert H, Bwanga F, Mukkada S, Nyesiga B, Julius PA, et al. (2010) Rapid screening of MDR-TB using molecular line probe assay is feasible in Uganda. BMC Infect Dis 10: 41.
11. Anek-vorapong R, Sinthuwattanawibool C, Podewills LJ, McCarthy K, Ngamlert K, et al. (2010) Validation of the GenoType® MTBDRplus assay for detection of MDR-TB in a public health laboratory in Thailand. BMC Infect Dis 10: 123.
12. Ling DI, Zwerling AA, Pai M, (2008) GenoType MTBDR assays for the diagnosis of multidrug-resistant tuberculosis: a meta-analysis. Eur Respir J 32: 1165-1174.
13. Raveendran R, Wattal C, Oberoi JK, Goel N, Datta S, et al. (2012) Utility of GenoType MTBDRplus assay in rapid diagnosis of multidrug resistant tuberculosis at a tertiary care centre in India. Indian J Med Microbiol 30: 58-63.
14. Huang WL, Chen HY, Kuo YM, Jou R, (2009) Performance indicator assessment of the GenoType MTBDRplus test and DNA sequencing in detection of multidrug-resistant Mycobacterium tuberculosis. J Clin Microbiol. 47: 2520-2524.
15. Boehme CC, Naveta P, Hillemann D, Nicol MP, Shenai S, et al. (2010) Rapid molecular detection of tuberculosis and rifampin resistance. N Eng J Med 363: 1005-1015.
16. World Health Organization (2010) Treatment of tuberculosis. Guidelines. Geneva (2010). WHO/HTM/TB/2009.420. WHO website. Available: http://whqlibdoc.who.int/publications/2010/9789241547833_eng.pdf. Accessed 2013 July 15.
17. Silva PE, Palomino JC, (2011) Molecular basis and mechanisms of drug resistance in Mycobacterium tuberculosis. Classical and new drugs. J Antimicrob Chemother 66: 1417-1430.
18. Mani C, Selvakumar N, Narayanan S, Narayanan PR, (2001) Mutations in rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from India. J Clin Microbiol 39: 2987-2990.
19. Cavusoglu C, Turhan A, Akinci P, Soyler I, (2006) Evaluation of the GenoType MTBDR Assay for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis isolates. J Clin Microbiol 44: 2238-2242.
20. Yue J, Shi W, Xie J, Li Y, Zeng E, et al. (2003) Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from China. J Clin Microbiol 41: 2209-2212.
21. Miotto P, Piana F, Penati V, Canducci F, Migliori GB, et al. (2006) Use of GenotypeMTBDR assay for molecular detection of rifampicin resistance in Mycobacterium tuberculosis clinical strains isolated in Italy. J Clin Microbiol 44: 2485-2491.
22. Tukvadze N, Kempker RR, Kalandadze L, Kurbatova E, Leonard MK, et al. (2012) Use of molecular diagnostic test in AFB smear positive tuberculosis suspects greatly reduces time to detection of multidrug-resistant tuberculosis. PLoS One 7(2): e31563.
23. Barnard M, Albert H, Coetzee G, O'Brien R, Bosman ME, (2008) Rapid molecular screening for multidrug-resistant tuberculosis in high-volume public health laboratory in South Africa. Am J Respir Crit Care Med 177: 787-792.
24. Kiepiela P, Bishop KS, Smith AN, Roux L, York DF, (2000) Genomic mutations in the katG, inhA and aphC genes are useful for the prediction of isoniazid resistance in Mycobacterium tuberculosis isolates from Kwazulu Natal, South Africa. Tuber Lung Dis 80: 47-56.
25. Tolani MP, Dsouza DT, Mistry NF, (2012) Drug resistance mutations and heteroresistance detected using the GenoTypeMTBDRplus assay and their implication for treatment outcomes in patients from Mumbai, India. BMC Infect Dis 12: 9.
26. Jacobson KR, Theron D, Victor TC, Streicher EM, Warren RM, et al. (2011) Treatment outcomes of Isoniazid resistant tuberculosis patients, western cap province, South Africa. Clin Infect Dis 53 (4): 369-372.
27. Causse M, Ruiz P, Zerolo J, Casal M, (2008) Evaluation of new GenoTypeMTBDRplus for detection of resistance in cultures and direct specimens of Mycobacterium tuberculosis.Int J Tuberc Lung Dis. 12: 1456-1460.
28. Dorman SE, Chihota VN, Lewis JJ, Meulen MVD, Mathema B, et al. (2009) GenoType MTBDRplus for direct detection of Mycobacterium tuberculosis and drug resistance in strains from gold miners in south Africa. J Clin Microbiol 50: 1189-1194.