Nuclear patch-clamp electrophysiology of Ca2+ channels

Cold Spring Harbor Protocols

Volumn 2013, Issue 9, 2013, Pages 885-891

  Mak, Don On Daniel ^a   Vais, Horia ^a   Cheung, King Ho ^b   Foskett, J Kevin ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM CHANNEL;

AMPLIFIER; ARTICLE; CELL ISOLATION; CELL MEMBRANE; CELL NUCLEUS MEMBRANE; CYTOPLASM; ELECTROPHYSIOLOGY; MICROMANIPULATOR; MICROPIPETTE; MICROSCOPY; PATCH CLAMP; PRESSURE TRANSDUCER;

CALCIUM; CALCIUM CHANNELS; CATIONS, DIVALENT; CELLS, CULTURED; CYTOLOGICAL TECHNIQUES; NUCLEAR ENVELOPE; PATCH-CLAMP TECHNIQUES;

EID: 84883476869     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot073064     Document Type: Article

References (7)

1. Bers D. M, Patton CW, Nuccitelli R. 2010. A practical guide to the preparation of Ca2+ buffers. Methods Cell Biol 99: 1-26.
2. Butler J.N. 1968. The thermodynamic activity of calcium ion in sodium chloride-calcium chloride electrolytes. Biophys J 8: 1426-1433.
3. Mak D.-OD, Vais H, Cheung K-H, Foskett JK. 2013a. Isolating nuclei from cultured cells for patch-clamp electrophysiology of intracellular Ca2+ channels. Cold Spring Harb Protoc doi: 10.1101/pdb.prot073056.
4. Mak D.-OD, Vais H, Cheung K-H, Foskett JK. 2013b. Patch-clamp electrophysiology of intracellular Ca2+ channels. Cold Spring Harb Protoc doi: 10.1101/pdb.top066217.
5. Penner R. 1995. A practical guide to patch-clamping. In Single-Channel Recording (ed. Sakmann B, Neher E), pp. 3-30. Plenum, New York.
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7. Vais H., Foskett JK, Mak D-OD. 2010. Unitary Ca2+ current through recombinant type 3 InsP3 receptor channels under physiological ionic conditions. J Gen Physiol 136: 687-700.