Brief report: Impaired cell reprogramming in nonhomologous end joining deficient cells

Stem Cells

Volumn 31, Issue 8, 2013, Pages 1726-1730

  Molina Estevez, F Javier ^a   Lozano, M Luz ^a   Navarro, Susana ^a   Torres, Yaima ^b   Grabundzija, Ivana ^c   Ivics, Zoltan ^d   Samper, Enrique ^b   Bueren, Juan A ^a   Guenechea, Guillermo ^a  

^a CIEMAT   (Spain)

^b CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES CNIC   (Spain)

^c MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^d PAUL EHRLICH INSTITUT   (Germany)

Author keywords

DNA dependent protein kinase catalytic subunit; Immunodeficiency; Immunodeficient mouse; Induced pluripotent stem cells; Nonhomologous end joining; Reprogramming; Severe combined

Indexed keywords

DNA DEPENDENT PROTEIN KINASE; LENTIVIRUS VECTOR;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; DNA END JOINING REPAIR; EMBRYO; FIBROBLAST; MOUSE; NONHUMAN; NUCLEAR REPROGRAMMING; PLURIPOTENT STEM CELL;

DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT; IMMUNODEFICIENT MOUSE; INDUCED PLURIPOTENT STEM CELLS; NONHOMOLOGOUS END JOINING; REPROGRAMMING; SEVERE COMBINED IMMUNODEFICIENCY;

ANIMALS; DNA END-JOINING REPAIR; INDUCED PLURIPOTENT STEM CELLS; MICE; MICE, INBRED BALB C; MICE, SCID; NUCLEAR REPROGRAMMING; TRANSFECTION;

EID: 84883442169     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.1406     Document Type: Article

References (24)

1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663-676.
2. Li H, Collado M, Villasante A et al. The Ink4/Arf locus is a barrier for iPS cell reprogramming. Nature 2009;460:1136-1139.
3. Marion RM, Strati K, Li H et al. A p53-mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity. Nature 2009;460:1149-1153.
4. Batista LF, Pech MF, Zhong FL et al. Telomere shortening and loss of self-renewal in dyskeratosis congenita induced pluripotent stem cells. Nature 2011;474:399-402.
5. Fong YW, Inouye C, Yamaguchi T et al. A DNA repair complex functions as an Oct4/Sox2 coactivator in embryonic stem cells. Cell 2011;147:120-131.
6. Raya A, Rodriguez-Piza I, Guenechea G et al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature 2009;460:53-59.
7. Muller LU, Milsom MD, Harris CE et al. Overcoming reprogramming resistance of Fanconi anemia cells. Blood 2012;119:5449-5457.
8. Kanaar R, Hoeijmakers JH, van Gent DC. Molecular mechanisms of DNA double strand break repair. Trends Cell Biol 1998;8:483-489.
9. Uematsu N, Weterings E, Yano K et al. Autophosphorylation of DNA-PKCS regulates its dynamics at DNA double-strand breaks. J Cell Biol 2007;177:219-229.
10. Kurimasa A, Kumano S, Boubnov NV et al. Requirement for the kinase activity of human DNA-dependent protein kinase catalytic subunit in DNA strand break rejoining. Mol Cell Biol 1999;19:3877-3884.
11. Allen C, Kurimasa A, Brenneman MA et al. DNA-dependent protein kinase suppresses double-strand break-induced and spontaneous homologous recombination. Proc Natl Acad Sci USA 2002;99: 3758-3763.
12. Somers A, Jean JC, Sommer CA et al. Generation of transgene-free lung disease-specific human induced pluripotent stem cells using a single excisable lentiviral stem cell cassette. Stem Cells 2010;28: 1728-1740.
13. Sommer CA, Stadtfeld M, Murphy GJ et al. Induced pluripotent stem cell generation using a single lentiviral stem cell cassette. Stem Cells 2009;27:543-549.
14. Mates L, Chuah MK, Belay E et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates. Nat Genet 2009;41:753-761.
15. Kues WA, Herrmann D, Barg-Kues B et al. Derivation and characterization of sleeping beauty transposon-mediated porcine induced pluripotent stem cells. Stem Cells Dev 2012;22:124-135.
16. Gilley D, Tanaka H, Hande MP et al. DNA-PKcs is critical for telomere capping. Proc Natl Acad Sci USA 2001;98:15084-15088.
17. Goytisolo FA, Samper E, Edmonson S et al. The absence of the dnadependent protein kinase catalytic subunit in mice results in anaphase bridges and in increased telomeric fusions with normal telomere length and G-strand overhang. Mol Cell Biol 2001;21:3642-3651.
18. Hande P, Slijepcevic P, Silver A et al. Elongated telomeres in scid mice. Genomics 1999;56:221-223.
19. Baekelandt V, Claeys A, Cherepanov P et al. DNA-dependent protein kinase is not required for efficient lentivirus integration. J Virol 2000; 74:11278-11285.
20. Daniel R, Greger JG, Katz RA et al. Evidence that stable retroviral transduction and cell survival following DNA integration depend on components of the nonhomologous end joining repair pathway. J Virol 2004;78:8573-8581.
21. Ivics Z, Hackett PB, Plasterk RH et al. Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell 1997;91:501-510.
22. Fulop GM, Phillips RA. The scid mutation in mice causes a general defect in DNA repair. Nature 1990;347:479-482.
23. Biedermann KA, Sun JR, Giaccia AJ et al. scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair. Proc Natl Acad Sci USA 1991;88: 1394-1397.
24. Araki R, Fujimori A, Hamatani K et al. Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice. Proc Natl Acad Sci USA 1997;94: 2438-2443