Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye

Nature Protocols

Volumn 8, Issue 9, 2013, Pages 1759-1764

  De La Rica, Roberto ^a   Stevens, Molly M ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CATALASE; GOLD; GOLD NANOPARTICLE; HYDROGEN PEROXIDE;

ANALYSIS; ANALYTE; ARTICLE; BIOCATALYSIS; CHEMICAL PHENOMENA; COLOR; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPERIMENTAL DESIGN; INTERMETHOD COMPARISON; PLASMONIC ELISA; PRIORITY JOURNAL; ULTRALOW CONCENTRATION;

CATALASE; ENZYME-LINKED IMMUNOSORBENT ASSAY; GOLD; HIV CORE PROTEIN P24; HYDROGEN PEROXIDE; METAL NANOPARTICLES; PROSTATE-SPECIFIC ANTIGEN; STREPTAVIDIN;

EID: 84883427366     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.085     Document Type: Article

References (15)

1. Clark, M.F., Lister, R.M. & Barjoseph, M. ELISA techniques. Method Enzymol. 118, 742-766 (1986).
2. Pierangeli, S.S. & Harris, E.N. A protocol for determination of anticardiolipin antibodies by ELISA. Nat. Protoc. 3, 840-848 (2008).
3. Ambrosi, A., Airo, F. & Merkoci, A. Enhanced gold nanoparticle based ELISA for a breast cancer biomarker. Anal. Chem. 82, 1151-1156 (2010).
4. Brena, B.M. et al. ELISA as an affordable methodology for monitoring groundwater contamination by pesticides in low-income countries. Environ. Sci. Technol. 39, 3896-3903 (2005).
5. Kang, X.P. et al. Development of an ELISA-array for simultaneous detection of five encephalitis viruses. Virol. J. 9, 56 (2012).
6. Neely, E. et al. Development of a capture/enrichment sandwich ELISA for the rapid detection of enteropathogenic and enterohaemorrhagic Escherichia coli O26 strains. J. Appl. Microbiol. 97, 1161-1165 (2004).
7. Asensio, L., Gonzalez, I., Garcia, T. & Martin, R. Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA). Food Control 19, 1-8 (2008).
8. Cuendet, M., Mesecar, A.D., DeWitt, D.L. & Pezzuto, J.M. An ELISA method to measure inhibition of the COX enzymes. Nat. Protoc. 1, 1915-1921 (2006).
9. Qu, W., Liu, Y., Liu, D., Wang, Z. & Jiang, X. Copper-mediated amplification allows readout of immunoassays by the naked eye. Angew. Chem. Int. Ed. 50, 3442-3445 (2011).
10. de la Rica, R. & Stevens, M.M. Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye. Nat. Nanotechnol. 7, 821-824 (2012).
11. Mei, Z.L. et al. Ultrasensitive one-step rapid visual detection of bisphenol A in water samples by label-free aptasensor. Biosens Bioelectron. 39, 26-30 (2013).
12. Zhou, Q.Q. et al. Development of gold nanoparticle-based rapid detection kit for melamine in milk products. J. Agric. Food Chem. 59, 12006-12011 (2011).
13. Yang, H. et al. A novel quantum dots-based point of care test for syphilis. Nanoscale Res. Lett. 5, 875-881 (2010).
14. Rodriguez-Lorenzo, L., de la Rica, R., Alvarez-Puebla, R.A., Liz-Marzan, L.M. & Stevens, M.M. Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth. Nat. Mater. 11, 604-607 (2012).
15. Engelbrekt, C. et al. Green synthesis of gold nanoparticles with starch-glucose and application in bioelectrochemistry. J. Mater. Chem. 19, 7839-7847 (2009).