Biolistics for high-throughput transformation and RNA interference in Drosophila melanogaster

Fly

Volumn 2, Issue 5, 2008, Pages 247-254

  Yuen, Jenna L ^a   Read, Scott A ^a   Brubacher, John L ^a,b   Singh, Aditi D ^a   Whyard, Steven ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

^b Canadian Mennonite University   (Canada)

Author keywords

Biolistics; Drosophila melanogaster; Genetic transformation; P element; PiggyBac; RNA interference

Indexed keywords

DROSOPHILA MELANOGASTER; HEXAPODA;

EID: 84883407467     PISSN: 19336934     EISSN: 19336942     Source Type: Journal    
DOI: 10.4161/fly.6985     Document Type: Article

References (48)

1. Sanford JC, Klein TM, Wolf ED, Allen N. Delivery of substances into cells and tissues using particle bombardment. Part Sci Technol 1987; 5:27-37.
2. Klein TM, Wolf ED, Wu R, Sanford JC. High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 1987; 327:70-3.
3. Serrano L, Rochange F, Semblat JP, Marque C, Teulieres C, Boudet AM. Genetic transformation of Eucalyptus globulus through biolistics: Complementary development of procedures for organogenesis from zygotic embryos and stable transformation of corresponding proliferating tissue. J Exp Botany 1996; 47:285-90.
4. Taylor N, Fauquet CM. Microparticle bombardment as a tool in plant science and agricultural biotechnology. DNA Cell Biol 2002; 21:963-77.
5. Lapidot M, Weil G, Cohen L, Segev L, Gaba V. Biolistic inoculation of plants with tomato yellow leaf curl virus DNA. J Virol Methods 2007; 144:143-8.
6. Rech EL, Vianna GR, Aragao FJ. High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants. Nat Protoc 2008; 3:410-18.
7. Jackstadt P, Wilm T, Zahner H, Hobom G. Transformation of nematodes via ballistic DNA transfer. Mol Biochem Parasitol 1999; 103:261-6.
8. Wilm T, Demel P, Koop H, Schnabel H, Schnabel R. Ballistic transformation of Caenorhabditis elegans. Gene 1999; 229:31-5.
9. Berezikov E, Bargmann CI, Plasterk RHA. Homologous gene targeting in Caenorhabditis elegans by biolistic transformation. Nucleic Acids Res 2004; 32:40.
10. Praitis V. Creation of transgenic lines using microparticle bombardment methods. Methods Mol Biol 2006; 351:93-107.
11. Gendreau S, Lardans V, Cadoret JP, Mialhe E. Transient expression of luciferase reporter gene after introduction into Artemia franciscana (Crustacea) embryos. Aquaculture 1995; 133:199-205.
12. Koster JG, Eizema K, Peterson-Maduro LJ, Stegeman BI, Destrée OH. Analysis of Wnt/ Engrailed signaling in Xenopus embryos using biolistics. Dev Biol 1996; 173:348-52.
13. Zelenin AV, Alimov AA, Zelenina IA, Semenova ML, Rodova MA, Chernov BK, Koleskinov VA. Transfer of foreign DNA into the cells of developing mouse embryos by microprojectile bombardment. FEBS Lett 1993; 315:29-32.
14. Haynes JR. Particle-mediated DNA vaccine delivery to the skin. Expert Opin Biol Ther 2004; 4:889-900.
15. O'Brien J, Lummis SC. Biolistic and diolistic transfection: using the gene gun to deliver DNA and lipophilic dyes into mammalian cells. Methods 2004; 33:121-5.
16. Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell 2005; 122:473-83.
17. O'Brien JA, Lummis SC. Biolistic transfection of neuronal cultures using a hand-held gene gun. Nat Protoc 2006; 1:977-81.
18. Maihle E, Miller L. Biolistic techniques for transfection of mosquito embryos (Anopheles gambiae). BioTechniques 1994; 16:924-31.
19. Thomas JL, Bardou J, L'hoste S, Mauchamp B, Chavancy G. A helium burst biolistic device adapted to penetrate fragile insect tissues. J Insect Sci 2001; 1:9.
20. Baldarelli M, Lengyel JA. Transient expression of DNA after ballistic introduction into Drosophila embryos. Nucleic Acids Res 1990; 18:5903-4.
21. Ryder E, Russell S. Transposable elements as tools for genomics and genetics in Drosophila. Brief Funct Genomic Proteomic 2003; 2:57-71.
22. Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA. Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and ciselement evolution. Genome Res 2005; 15:1-18.
23. Drosophila 12 Genomes Consortium, 2007 Evolution of genes and genomes on the Drosophila phylogeny. Nature 450:203-18.
24. Stark A, Lin MF, Kheradpour P, Pedersen JS, Parts L, Carlson JW, Crosby MA, Rasmussen MD, Roy S, Deoras AN, Ruby JG, Brennecke J; Harvard FlyBase curators; Berkeley Drosophila Genome Project, Hodges E, Hinrichs AS, Caspi A, Paten B, Park SW, Han MV, Maeder ML, Polansky BJ, Robson BE, Aerts S, van Helden J, Hassan B, Gilbert DG, Eastman DA, Rice M, Weir M, Hahn MW, Park Y, Dewey CN, Pachter L, Kent WJ, Haussler D, Lai EC, Bartel DP, Hannon GJ, Kaufman TC, Eisen MB, Clark AG, Smith D, Celniker SE, Gelbart WM, Kellis M. Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures. Nature 2007; 450:219-32.
25. Berezikov E, Bargmann CI, Plasterk RH. Homologous gene targeting in Caenorhabditis elegans by biolistic transformation. Nucleic Acids Res 2004; 32:40.
26. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 1967; 26:365-9.
27. Fraser MJ. The TTAA-specific family of transposable elements. In: James AA, Handler AH, eds. Insect Transgenesis: Methods and Applications. Orlando; CRC Press 2000; 249-68.
28. Hannon GJ. RNA interference. Nature 2002; 418:244-51.
29. Dubreuil RR, Wang P. Genetic analysis of the requirements for α-actinin function. J Muscle Res Cell Motility 2000; 21:705-13.
30. Chen Q, Ma E, Behar KL, Xu T, Haddad GG. Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster. J Biol Chem 2002; 277:32749.
31. Handler AM. Use of the piggyBac transposon for germ-line transformation of insects. Insect Biochem Mol Biol 2002; 32:1211-20.
32. Sijen T, Fleenor J, Simmer F, Thijssen KL, Parrish S, Timmons L, Plasterk RH, Fire A. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell 2001; 107:465-76.
33. Schiebel W, Pélissier T, Riedel L, Thalmeir S, Schiebel R, Kempe D, Lottspeich F, Sänger HL, Wassenegger M. Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato. Plant Cell 1998; 10:2087-101.
34. Dalmay T, Hamilton A, Rudd S, Angell S, Baulcombe DC. An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell 2000; 101:543-53.
35. Horiuchi H, Udagawa T, Koga R, Moriyama H, Fukuhara T. RNA-dependent RNA polymerase activity associated with endogenous double-stranded RNA in rice. Plant Cell Physiol 2001; 42:197-203.
36. Yu D, Fan B, MacFarlane SA, Chen Z. Analysis of the involvement of an inducible Arabidopsis RNA-dependent RNA polymerase in antiviral defense. Mol Plant Microbe Interact 2003; 16:206-16.
37. Lipardi C, Wei Q, Paterson BM. RNAi as random degradative PCR: siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs. Cell 2001; 107:297-307.
38. FlyBase Consortium. The FlyBase database of the Drosophila genome projects and community literature. Nucleic Acids Res 2003; 31:172-5.
39. Spradling AC, Rubin GM. Transposition of cloned P elements into Drosophila germ-line chromosomes. Science 1982; 218:341-7.
40. Steller H, Pirrotta V. A transposable P vector that confers selectable G418 resistance to Drosophila larvae. EMBO J 1985; 4:167-71.
41. Horn C, Wimmer EA. A versatile vector set for animal transgenesis. Dev Genes Evol 2000; 210:630-7.
42. Jowett T. Preparation of nucleic acids In: Roberts DB, ed. Drosophila: A Practical Approach. Oxford; Oxford University Press 1998; 347-71.
43. Rubin GM, Spradling AC. Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res 1983; 11:6341-51.
44. Horn C, Offen N, Nystedt S, Häcker U, Wimmer EA. piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. Genetics 2003; 163:647-61.
45. Gallagher SR. Quantification of GUS activity by fluorometry. In: Gallagher SR, ed. GUS protocols: using the GUS gene as a reporter of gene expression. San Diego; Academic Press 1992; 47-60.
46. Carthew RW. RNAi applications in Drosophila melanogaster. In: Hannon GJ, ed. RNAi-A Guide to Gene Silencing. New York, NY: Cold Spring Harbor Laboratory Press 2003; 361-400.
47. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29:45.
48. Schulze U, Larsen ME, Villadsen J. Determination of intracellular trehalose and glycogen in Saccharomyces cerevisiae. Anal Biochem 1995; 228:143-9.