On the detection of anti-HLA antibodies using single antigen bead luminex assay: Lot-to-lot variations in MFI

Transplantation

Volumn 96, Issue 4, 2013, Pages

  Friedlander, Rex ^a   Putheti, Prabhakar ^a,b   Diaz, Elder ^a   Menon, Arvind ^a   Ponce, Blanca ^a   Muthukumar, Thangamani ^b   Sharma, Vijay K ^a,b   Dadhania, Darshana ^a,b   Suthanthiran, Manikkam ^a,b  

^a ROGOSIN INSTITUTE   (United States)

^b WEILL CORNELL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; HLA ANTIBODY; IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY W6 32; UNCLASSIFIED DRUG;

ANTIBODY DETECTION; ANTIGEN BINDING; COMPARATIVE STUDY; CORRELATION COEFFICIENT; HUMAN; IMMUNOASSAY; KIDNEY TRANSPLANTATION; LETTER; MEAN FLUORESCENCE INTENSITY; MEASUREMENT PRECISION; PHYSICAL PARAMETERS; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; GRAFT REJECTION; HLA ANTIGENS; HUMANS; IMMUNOASSAY; ISOANTIBODIES; KIDNEY TRANSPLANTATION; REFERENCE STANDARDS; TISSUE DONORS;

EID: 84883397940     PISSN: 00411337     EISSN: None     Source Type: Journal    
DOI: 10.1097/TP.0b013e31829c2481     Document Type: Letter

References (7)

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2. Lachmann N, Todorova K, Schulze H, et al. Systemic comparison of four cell- and Luminex-based methods for assessment of complement-activating HLA antibodies. Transplantation 2013; 95: 1.
3. Tait B, Caner S, Gebel H, et al. Consensus guidelines on the testing and clinical management issues associated with HLA and non-HLA antibodies in transplantation. Transplantation 2013; 95: 19.
4. Rao P, Zhang Z, Gebel H, et al. Validation of the Luminex platform to detect and quantify HLA antibodies: Effect of standardizing operation procedures on test variability byCTOT core laboratories. Hum Immunol 2012; 73: 25.
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6. Gandhi MJ, Carrick DM, Jenkins S, et al. Lot-to-lot variability in HLA antibody screening using a multiplexed bead-based assay. Transfusion 2013; [epub ahead of print doi: 10.1111/trf.12064].
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