Generation of a Tn5 transposon library in Haemophilus parasuis and analysis by transposon-directed insertion-site sequencing (TraDIS)

Veterinary Microbiology

Volumn 166, Issue 3-4, 2013, Pages 558-566

  Luan, Shi Lu ^a   Chaudhuri, Roy R ^a,f   Peters, Sarah E ^a   Mayho, Matthew ^b   Weinert, Lucy A ^a   Crowther, Sarah A ^a   Wang, Jinhong ^a   Langford, Paul R ^c   Rycroft, Andrew ^d   Wren, Brendan W ^e   Tucker, Alexander W ^a   Maskell, Duncan J ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d ROYAL VETERINARY COLLEGE   (United Kingdom)

^e LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^f UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Haemophilus parasuis; Tn5 mutagenesis; TraDIS

Indexed keywords

CHLORAMPHENICOL; DNA; KANAMYCIN; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CONTROLLED STUDY; DNA LIBRARY; DNA MODIFICATION; ELECTROPORATION; ELECTROTRANSFORMATION; GENE CONSTRUCT; GENE DISRUPTION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC MANIPULATION; HAEMOPHILUS PARASUIS; MOLECULAR PATHOLOGY; MUTANT; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PROMOTER REGION; SEROTYPE; SHUTTLE VECTOR; SITE DIRECTED MUTAGENESIS; TRANSPOSON; TRANSPOSON DIRECTED INSERTION SITE SEQUENCING;

BACTERIA (MICROORGANISMS); HAEMOPHILUS PARASUIS; SUIDAE;

HAEMOPHILUS PARASUIS; TN5 MUTAGENESIS; TRADIS;

ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; ELECTROPORATION; GENE LIBRARY; HAEMOPHILUS PARASUIS; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL;

EID: 84883303276     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2013.07.008     Document Type: Article

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