Primary culture and live imaging of adult neural stem cells and their progeny

Methods in Molecular Biology

Volumn 1052, Issue , 2013, Pages 1-11

  Ortega, Felipe ^a   Berninger, Benedikt ^a   Costa, Marcos R ^b  

^a UNIVERSITY OF MUNICH   (Germany)

^b FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE   (Brazil)

Author keywords

Adult neural stem cell; Astroglia; Cell culture; Neurogenesis; Subependymal zone; Time lapse video microscopy

Indexed keywords

ADULT STEM CELL; ANIMAL CELL; ARTICLE; BRAIN; CELL CULTURE; CELL CYCLE; IMAGING; LIVE IMAGING; MACROGLIA; METHODOLOGY; MOUSE; NERVE CELL; NERVOUS SYSTEM DEVELOPMENT; NEURAL STEM CELL; NONHUMAN; OLFACTORY BULB; PRIORITY JOURNAL; SUBVENTRICULAR ZONE; TIME LAPSE IMAGING; VIDEORECORDING;

ADULT STEM CELLS; ANIMALS; ASTROCYTES; BRAIN; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CELL PROLIFERATION; CELLS, CULTURED; MICE; NEURAL STEM CELLS; NEUROGENESIS; NEURONS;

EID: 84883230332     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651-2013-22     Document Type: Article

References (19)

1. Zhao C, Deng W, Gage FH (2008) Mechanisms and functional implications of adult neurogenesis. Cell 132:645-660
2. Alonso M, Ortega-Perez I, Grubb MS et al(2008)Turning astrocytes from the rostralmigratory stream into neurons: A role for the olfactory sensory organ. J Neurosci 28:11089-11102
3. Merkle FT, Mirzadeh Z, Alvarez-Buylla A(2007) Mosaic organization of neural stem cells in the adult brain. Science 317:381-384
4. BrillMS, SnapyanM,WohlfromHet al (2008)A dlx2- and pax6-dependent transcriptional code for periglomerular neuron specification in the adult olfactory bulb. J Neurosci 28:6439-6452
5. Brill MS, Ninkovic J, Winpenny E et al (2009) Adult generation of glutamatergic olfactory bulb interneurons.NatNeurosci 12:1524-1533
6. Carleton A, Petreanu LT, Lansford R et al(2003) Becoming a new neuron in the adult olfactory bulb. Nat Neurosci 6:507-518
7. Hack MA, Saghatelyan A, de Chevigny A et al(2005)Neuronal fate determinants of adultolfactorybulbneurogenesis. NatNeurosci8:865-872
8. Lledo PM, Merkle FT, Alvarez-Buylla A (2008) Origin and function of olfactory bulb interneuron diversity. Trends Neurosci 31:392-400
9. Rietze RL, Reynolds BA (2006) Neural stem cell isolation and characterization. Methods Enzymol 419:3-23
10. Chojnacki A, Weiss S (2008) Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells. Nat Protoc 3:935-940
11. Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255:1707-1710
12. Reynolds BA, Rietze RL (2005) Neural stem cells and neurospheres- re-evaluating the relationship. Nat Methods 2:333-336
13. Doetsch F, Petreanu L, Caille I et al (2002) EGF converts transit-Amplifying neurogenic precursors in the adult brain into multipotent stem cells. Neuron 36:1021-1034
14. Golmohammadi MG, Blackmore DG, Large B et al (2008) Comparative analysis of the frequency and distribution of stem and progenitor cells in the adult mouse brain. Stem Cells 26:979-987
15. Luskin MB (1993) Restricted proliferation and migration of postnatally generated neurons derived from the forebrain subventricular zone. Neuron 11:173-189
16. Kuhn HG, Winkler J, Kempermann G et al(1997) Epidermal growth factor and fibroblast growth factor-2 have different effects on neural progenitors in the adult rat brain. J Neurosci 17:5820-5829
17. Lim DA, Alvarez-Buylla A (1999) Interaction between astrocytes and adult subventricular zone precursors stimulates neurogenesis. Proc Natl Acad Sci U S A 96:7526-7531
18. Scheffler B, Walton NM, Lin DD et al (2005) Phenotypic and functional characterization of adult brain neuropoiesis. Proc Natl Acad Sci U S A 102:9353-9358
19. Costa MR, Ortega F, Brill MS et al (2011) Continuous live imaging of adult neural stem cell division and lineage progression in vitro. Development 138:1057-1068