Biotechnological applications of supersonic cluster beam-deposited nanostructured thin films: Bottom-up engineering to optimize cell-protein-surface interactions

Journal of Biomedical Materials Research - Part A

Volumn 101, Issue 10, 2013, Pages 2994-3008

  Singh, Ajay Vikram ^a,b  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

AFM; cell surface interactions; high throughput; microarray; nanocluster

Indexed keywords

AFM; BIOTECHNOLOGICAL APPLICATIONS; CELL-SURFACE INTERACTIONS; HIGH THROUGHPUT; NANOSTRUCTURED COATINGS; NANOSTRUCTURED THIN FILM; PROTEIN-SURFACE INTERACTIONS; TECHNOLOGICAL INNOVATION;

BIOCOMPATIBILITY; BIOLOGICAL MATERIALS; BIOTECHNOLOGY; COATINGS; DEPOSITION; MAMMALS; MICROARRAYS; NANOCLUSTERS;

CELL ENGINEERING;

GOLD; NANOCOATING; NANOFILM; NANOMATERIAL; SILVER; TITANIUM DIOXIDE; ZIRCONIUM OXIDE;

ADSORPTION; ANTIGEN DETECTION; BACTERIAL COLONIZATION; BIOCOMPATIBILITY; BIOTECHNOLOGY; CANCER CELL CULTURE; CARCINOGENESIS; CELL INTERACTION; DYNAMICS; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX; FILM; FLUID FLOW; GENOTOXICITY; HIGH THROUGHPUT SCREENING; HUMAN; IMMUNOFLUORESCENCE; LANGMUIR BLODGETT FILM; NANOFABRICATION; NANOMEDICINE; NONHUMAN; OSTEOSARCOMA CELL; PHASE CONTRAST MICROSCOPY; PHEOCHROMOCYTOMA; PHYSICAL CHEMISTRY; POLARIZATION; POROSITY; PROTEIN IMMOBILIZATION; PROTEIN INTERACTION; PROTEIN MICROARRAY; PROTEOMICS; REVIEW; RNA INTERFERENCE; STEM CELL EXPANSION; TISSUE ENGINEERING; WESTERN BLOTTING; WETTABILITY;

AFM; CELL-SURFACE INTERACTIONS; HIGH THROUGHPUT; MICROARRAY; NANOCLUSTER;

ANIMALS; BIOTECHNOLOGY; CELLS; GASES; HUMANS; NANOSTRUCTURES; NANOTECHNOLOGY; PROTEINS;

EID: 84883220787     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.34601     Document Type: Review

References (116)

1. Santamaria A,. Historical overview of nanotechnology and nanotoxicology. Methods Mol Biol 2012; 926: 1-12.
2. Nano-express from New Delhi? Nat Mater 2005; 4 (8): 575.
3. Zhou J, Wu S, Yu T, Xie Z,. 2011 International Conference on Modified Pyrolytic Carbon Black from Scrap Tires and Its Reinforcement Performance in Natural Rubber. Computer Distributed Control and Intelligent Environmental Monitoring (CDCIEM); 2011. p 472-475.
4. 2 pigment technology: A review. Prog Org Coat 1992; 20 (2):105-138.
5. Ulrike D,. The surface science of titanium dioxide. Surf Sci Rep 2003; 48 (5-8): 53-229.
6. Eran B,. In:, Eitan S, Shimon U, editors. Combining Top-Down and Bottom-Up Segmentation; 2004. p 46.
7. Eaton MAW,. How do we develop nanopharmaceuticals under open innovation? Nanomed Nanotechnol Biol Med 2011; 7 (4): 371-375
8. Neher E,. Molecular biology meets microelectronics. Nat Biotechnol 2001; 19 (2): 114
9. Singh AV, Ferri M, Tamplenizza M, Borghi F, Divitini G, Ducati C, Lenardi C, Piazzoni C, Merlini M, Podestà A, Milani P,. Bottom-up engineering of the surface roughness of nanostructured cubic zirconia to control cell adhesion. Nanotechnology 2012; 23: 475101.
10. Perry JL, Herlihy KP, Napier ME, DeSimone JM,. PRINT: A novel platform toward shape and size specific nanoparticle theranostics. Acc Chem Res 2011; 44 (10): 990-998
11. Mihail CR,. Nanotechnology: Convergence with modern biology and medicine. Curr Opin Biotechnol 2003; 14 (3): 337-346
12. Wilson CJ, Clegg RE, Leavesley DI, Pearcy MJ,. Mediation of biomaterial-cell interactions by adsorbed proteins: A review. Tissue Eng 2005; 11 (1-2): 1-18.
13. Yarmush ML, King KR,. Living-cell microarrays. Annu Rev Biomed Eng 2009; 11 (1): 235-257
14. Galli C, Collaud Coen M, Hauert R, Katanaev VL, Wymann MP, Gröning P, Schlapbach L. Protein adsorption on topographically nanostructured titanium. Surf Sci 2001; 474 (1-3): L180-L184.
15. Singh AV, Vyas V, Montani E, Cartelli D, Parazzoli D, Oldani A, Zeri G, Orioli E, Gemmati D, Zamboni P,. Investigation of in vitro cytotoxicity of the redox state of ionic iron in neuroblastoma cells. J Neurosci Rural Pract 2012; 3 (3): 301-310
16. Li J,. Nanostructured Biomaterials. New York, NY: Springer; 2010. p 222.
17. Singh AV, Khare M, Gade WN, Zamboni P,. Theranostic implications of nanotechnology in multiple sclerosis: A future perspective. Autoimmune Dis 2012; 2012: 1-12.
18. Vendra VK, Lin Wu, Krishnan S,. Polymer Thin Films for Biomedical Applications. Weinheim, Germany: Wiley-VCH Verlag; 2010. p 54.
19. Boccaccini AR, Keim S, Ma R, Li Y, Zhitomirsky I,. Electrophoretic deposition of biomaterials. J R Soc Interface 2010; 7 (Suppl 5): S581-S613.
20. Ariga K, Nakanishi T, Michinobu T,. Immobilization of biomaterials to nano-assembled films (self-assembled monolayers, Langmuir-Blodgett films, and layer-by-layer assemblies) and their related functions. J Nanosci Nanotechnol 2006; 6 (8): 2278-2301
21. Singh AV, Patil R, Kasture MB, Gade WN, Prasad BL,. Synthesis of Ag-Pt alloy nanoparticles in aqueous bovine serum albumin foam and their cytocompatibility against human gingival fibroblasts. Colloids Surf B: Biointerfaces 2009; 69 (2): 239-245
22. Singh AV, Bandgar BM, Kasture M, Prasad BLV, Sastry M,. Synthesis of gold, silver and their alloy nanoparticles using bovine serum albumin as foaming and stabilizing. J Mater Chem 2005; 15 (48): 5115-5121
23. Stark WJ, Pratsinis SE,. Aerosol flame reactors for manufacture of nanoparticles. Powder Technol 2002; 126 (2): 103-108
24. Wegner K, Pratsinis SE,. Scale-up of nanoparticle synthesis in diffusion flame reactors. Chem Eng Sci 2003; 58 (20): 4581-4589
25. Kammler HK, Mädler L, Pratsinis SE,. Flame synthesis of nanoparticles. Chem Eng Technol 2001; 24 (6): 583-596
26. Singh Y, Javier RN, Ehrman SH, Magnusson MH, Deppert K,. Approaches to increasing yield in evaporation/condensation nanoparticle generation. J Aerosol Sci 2002; 33 (9): 1309-1325
27. Wegner K, Pratsinis SE,. Nozzle-quenching process for controlled flame synthesis of titania nanoparticles. AIChE J 2003; 49 (7) 1667-1675
28. Milani P, Barborini E, Piseri P, Bottani CE, Ferrari AC, Li Bassi A,. Nanostructured carbon films from supersonic cluster beam deposition: Structure and morphology. Eur Phy J D 1999; 9 (1): 63-68
29. Milani P, Piseri P, Barborini E, Podesta A, Lenardi C,. Cluster beam synthesis of nanostructured thin films. J Vac Sci Technol 2001; 19 (4): 2025-2033
30. Mazza T, Barborini E, Kholmanov IN, Piseri P, Bongiorno G, Vinati S, Milani P, Ducati C, Cattaneo D, Li Bassi A, Bottani CE, Taurino AM, Siciliano P,. Libraries of cluster-assembled titania films for chemical sensing. Appl Phys Lett 2005; 87: 103108.
31. Piseri P, Podesta A, Barborini E, Milani P,. Production and characterization of highly intense and collimated cluster beams by inertial focusing in supersonic expansions. Rev Sci Instrum 2001; 72 (5): 2261-2267
32. Tafreshi HV, Piseri P, Benedek G, Milani P,. The role of gas dynamics in operation conditions of a pulsed microplasma cluster source for nanostructured thin films deposition. J Nanosci Nanotechnol 2006; 6: 1140-1149.
33. Mazza T, Barborini E, Kholmanov IN, Piseri P, Bongiorno G, Vinati S, MIlani P, Ducati C, Li Bassi A, Bottani CE, Taurino AM, Siciliano P. Libraries of cluster-assembled titania films for chemical sensing. Appl Phys Lett 2005; 87 (10).
34. Wegner K, Piseri P, Tafreshi V, Milani P,. Cluster beam deposition: A tool for nanoscale science and technology. J Phys D: Appl Phys 2006; 39 (22): R439-R459.
35. Chiappini C, Piseri P, Vinati S, Milani P,. Supersonic cluster beam deposition of nanostructured thin films with uniform thickness via continuously graded exposure control. Rev Sci Instrum 2007; 78 (6).
36. Barborini E, Piseri P, Milani P,. A pulsed microplasma source of high intensity supersonic carbon cluster beams. J Phys D: Appl Phys 1999; 32: L105.
37. Barborini E, Piseri P, Milani P,. A pulsed microplasma source of high intensity supersonic carbon cluster beams. J Phys D: Appl Phys 1999; 32: L105-L109.
38. Andersen N,. In:, Scoles G, editor. Atomic and Molecular Beam Methods, Vol. 1. New York, NY and Oxford, UK: Oxford University Press; 1988. p 206-207.
39. Barborini E, Piseri P, Li Bassi A, Ferrari AC, Bottani CE, Milani P,. Synthesis of carbon films with controlled nanostructure by separation of neutral clusters in supersonic beams. Chem Phys Lett 1999; 300 (5): 633-638
40. Tafreshi HV, Benedek G, Piseri P, Vinati S, Barborini E, Milani P,. A simple nozzle configuration for the production of low divergence supersonic cluster beam by aerodynamic focusing. Aerosol Sci Technol 2002; 36 (5): 593-606
41. Milani P, Piseri P, Barborini E, Podesta A, Lenardi C,. Cluster beam synthesis of nanostructured thin films. J Vac Sci Technol A: Vac Surf Films 2001; 19: 2025.
42. Barborini E, Piseri P, Podesta A, Milani P,. Cluster beam microfabrication of patterns of three-dimensional nanostructured objects. Appl Phys Lett 2000; 77 (7): 1059-1061
43. Piseri P, Tafreshi HV, Milani P,. Manipulation of nanoparticles in supersonic beams for the production of nanostructured materials. Curr Opin Solid State Mater Sci 8: 195-202.
44. Singh AV, Vyas V, Patil R, Sharma V, Scopelliti PE, Bongiorno G, Podestà A, Lenardi C, Gade WN, Milani P. Quantitative characterization of the influence of the nanoscale morphology of nanostructured surfaces on bacterial adhesion and biofilm formation. PLoS One 2011; 6 (9): e25029.
45. Singh AV, Salve TS, Cortelli D, Dellasega D, Podestã A, Milani P, Gade WN,. Biofilm formation on nanostructured titanium oxide surfaces and a micro/nanofabrication-based preventive strategy using colloidal lithography. Biofabrication 2012; 4 (3).
46. Fioretti F, Mendoza-, Palomares C, Helms M, Al, Alam D, Richert L, Arntz Y, Rinckenbach S, Garnier F, Haïkel Y, Gangloff SC, Benkirane-Jessel N. Nanostructured assemblies for dental application. ACS Nano 2010; 4 (6): 3277-3287
47. Barborini E, Vinati S, Leccardi M, Repetto P, Bertolini G, Rorato O, Lorenzelli L, Decarli M, Guarnieri V, Ducati C, Milani P,. Batch fabrication of metal oxide sensors on micro-hotplates. J Micromech Microeng 2008; 18: 7.
48. Cassina V, Gerosa L, Podestà A, Ferrari G, Sampietro M, Fiorentini F, Mazza T, Lenardi C, Milani P. Nanoscale electrical properties of cluster-assembled palladium oxide thin films. Phys Rev B 2009; 79 (11): 115422
49. 2 films produced by supersonic cluster beam deposition. J Nanopart Res 2009; 11 (6): 1339-1348
50. Carbone R, De, Marni M, Zanardi A, Vinati S, Barborini E, Fornasari L, Milani P. Characterization of cluster-assembled nanostructured titanium oxide coatings as substrates for protein arrays. Anal Biochem 2009; 394 (1): 7-12
51. 2 with primary and cancer cells. Biomaterials 2006; 27 (17) 3221-3329
52. 2 Films with 2 eV optical gap. Adv Mater 2005; 17 (15): 1842-1846
53. Vyas V, Podestà A, Milani P,. Probing nanoscale interactions on biocompatible cluster-assembled titanium oxide surfaces by atomic force microscopy. J Nanosci Nanotechnol 2011; 11 (6): 4739-4748
54. 2 grown by cluster assembling. Surf Sci 2007; 601 (13): 2688-2691
55. Vandamme N, Verschoren G, Depuydt A, Cannaerts M, Bouwen W, Lievens P, Silverans CE, Van Haesendonck C. Deposition of gold clusters on atomically flat gold films. Appl Phys A: Mater Sci Proc 2001; 72 (8): S177-S180.
56. Barabasi A, Stanley H,. Fractal Concepts in Surface Growth-Cambridge Books Online. New York: Cambridge University Press; 1995.
57. 2. J Phys Chem B 2006; 110 (20): 9995-10001
58. Giorgetti L, Bongiorno G, Podestà A, Berlanda G, Scopelliti PE, Carbone R, Milani P. Adsorption and stability of streptavidin on cluster-assembled nanostructured TiOx films. Langmuir 2008; 24 (20): 11637-11644
59. Fabre RM, Talham DR,. Stable supported lipid bilayers on zirconium phosphonate surfaces. Langmuir 2009; 25 (21): 12644-12652
60. Denry I, Kelly JR,. State of the art of zirconia for dental applications. Dent Mater 2008; 24 (3): 299-307
61. Ratner B,. The biocompatibility manifesto: biocompatibility for the twenty-first century. J CardiovasTransl Res 2011: 1-5.
62. Oliver RG,. In:, Brantley WA, Eliades T, editors. Orthodontic Materials. Scientific and Clinical Aspects. Stuttgart, Germany: Thieme; 2001. p 310.
63. Singh AV, Lenardi C, Gailite L, Gianfelice A, Milani P,. A simple lift-off-based patterning method for micro- and nanostructuring of functional substrates for cell culture. J Micromech Microeng 2009; 19 (11): 115028
64. Singh AV, Gailite L, Vyas V, Lenardi C, Forti S, Matteoli M, Milani P. Rapid prototyping of nano- and micro-patterned substrates for the control of cell neuritogenesis by topographic and chemical cues. Mater Sci Eng C 2011; 31 (5): 892-899
65. Schmidt SR, Waldeck H, Kao WJ,. Protein Adsorption to Biomaterials. New York, NY: Springer; 2009. p 1-18.
66. Koegler P, Clayton A, Thissen H, Santos GN, Kingshott P,. The influence of nanostructured materials on biointerfacial interactions. Adv Drug Deliv Rev 2012; 64 (15): 1820-1839
67. Scopelliti PE, Borgonovo A, Indrieri M, Giorgetti L, Bongiorno G, Carbone R, Podestà A, Milani P. The effect of surface nanometre-scale morphology on protein adsorption. PLoS One 2010; 5 (7): e11862.
68. 2. Biomaterials 2010; 31 (20): 5385-5396
69. Scopelliti PE, Giorgetti L, Bongiorno G, Podestá A, Berlanda G, Carbone R, Milani P. Adsorption and stability of streptavidin on cluster-assembled nanostructured TiOx films. Biophys J 2009; 96 (3, Suppl 1): 50a-a.
70. Giorgetti L, Zanardi A, Venturini S, Carbone R,. Immunocell-array: A novel technology for pathway discovery and cell profiling. Expert Rev Proteomics 2007; 4 (5): 609-616
71. Wheeler DB, Carpenter AE, Sabatini DM,. Cell microarrays and RNA interference chip away at gene function. Nat Genet 2005; 37 (6 Suppl): S25-S30.
72. Bailey SN, Ali SM, Carpenter AE, Higgins CO, Sabatini DM,. Microarrays of lentiviruses for gene function screens in immortalized and primary cells. Nat Methods 2006; 3 (2): 117-122
73. Wu RZ, Bailey SN, Sabatini DM,. Cell-biological applications of transfected-cell microarrays. Trends Cell Biol 2002; 12 (10): 485-488
74. Wheeler DB, Bailey SN, Guertin DA, Carpenter AE, Higgins CO, Sabatini DM,. RNAi living-cell microarrays for loss-of-function screens in Drosophila melanogaster cells. Nat Methods 2004; 1 (2): 127-132
75. Ziauddin J, Sabatini DM,. Microarrays of cells expressing defined cDNAs. Nature 2001; 411 (6833): 107-110
76. Hubbell JA,. Materials as morphogenetic guides in tissue engineering. Curr Opin Biotechnol 2003; 14 (5): 551-558
77. Stevens MM, George JH,. Exploring and engineering the cell surface interface. Science 2005; 310 (5751): 1135-1138
78. Haouzi D, Baghdiguian S, Granier G, Travo P, Mangeat P, Hibner U,. Three-dimensional polarization sensitizes hapatocytes to Fas/CD95 apoptotic signalling. J Cell Sci 2005; 118 (12): 2763-2773
79. Hook AL, Thissen H, Voelcker NH,. Surface manipulation of biomolecules for cell microarray applications. Trends Biotechnol 2006; 24 (10): 471-477
80. Falconnet D, Csucs G, Michelle Grandin H, Textor M,. Surface engineering approaches to micropattern surfaces for cell-based assays. Biomaterials 2006; 27 (16): 3044-3063
81. Echeverri CJ, Perrimon N,. High-throughput RNAi screening in cultured cells: A user's guide. Nat Rev Genet 2006; 7 (5): 373-384
82. Jeffrey AH,. Materials as morphogenetic guides in tissue engineering. Curr Opin Biotechnol 2003; 14 (5): 551-558
83. Haouzi D, Baghdiguian S, Granier G, Travo P, Mangeat P, Hibner U,. Three-dimensional polarization sensitizes hepatocytes to Fas/CD95 apoptotic signalling. J Cell Sci 2005; 118 (12): 2763-2773
84. 2 for functional genomics and drug discovery. Biomaterials 2007; 28 (13): 2244-2253
85. Legrain P, Aebersold R, Archakov A, Bairoch A, Bala K, Beretta L, Bergeron J, Borchers CH, Corthals GL, Costello CE, Deutsch EW, Domon B, Hancock W, He F, Hochstrasser D, Marko-Varga G, Salekdeh GH, Sechi S, Snyder M, Srivastava S, Uhlén M, Wu CH, Yamamoto T, Paik YK, Omenn GS. The human proteome project: current state and future direction. Mol Cell Proteomics 2011; 10 (7).
86. Hanash SM, Pitteri SJ, Faca VM,. Mining the plasma proteome for cancer biomarkers. Nature 2008; 452 (7187): 571-579
87. Harrison PM, Kumar A, Lang N, Snyder M, Gerstein M,. A question of size: The eukaryotic proteome and the problems in defining it. Nucleic Acids Res 2002; 30 (5): 1083-1090
88. Bertone P, Snyder M,. Advances in functional protein microarray technology. FEBS J 2005; 272 (21): 5400-5411
89. Saerens D, Ghassabeh GH, Muyldermans S,. Antibody technology in proteomics. Brief Funct Genomics Proteomics 2008; 7 (4): 275-282
90. Ling MM, Ricks C, Lea P,. Multiplexing molecular diagnostics and immunoassays using emerging microarray technologies. Expert Rev Mol Diagn 2007; 7 (1): 87-98
91. Saerens D, Ghassabeh GH, Muyldermans S,. Antibody technology in proteomics. Brief Funct Genomics Proteomics 2008; 7 (4): 275-282
92. Kasemo B, Gold J,. Implant surfaces and interface processes. Adv Dent Res 1999; 13 (1): 8-20
93. Zanardi A, Giorgetti L, Botrugno OA, Minucci S, Milani P, Pelicci PG, Carbone R,. Immunocell-array for molecular dissection of multiple signaling pathways in mammalian cells. Mol Cell Proteomics 2007; 6 (5): 939-947
94. Sevecka M, Wolf-, Yadlin A, MacBeath G,. Lysate microarrays enable high-throughput, quantitative investigations of cellular signaling. Mol Cell Proteomics 2011; 10 (4): M110.005363.
95. Liotta LA, Kohn EC, Petricoin EF,. Clinical Proteomics. JAMA 2001; 286 (18): 2211-2214
96. Petricoin EF, 3rd, Bichsel VE, Calvert VS, Espina V, Winters M, Young L, Belluco C, Trock BJ, Lippman M, Fishman DA, Sgroi DC, Munson PJ, Esserman LJ, Liotta LA. Mapping molecular networks using proteomics: A vision for patient-tailored combination therapy. J Clin Oncol 2005; 23 (15): 3614-3621
97. Spurrier B, Ramalingam S, Nishizuka S,. Reverse-phase protein lysate microarrays for cell signaling analysis. Nat Protoc 2008; 3 (11): 1796-1808
98. Chan SM, Ermann J, Su L, Fathman CG, Utz PJ,. Protein microarrays for multiplex analysis of signal transduction pathways. Nat Med 2004; 10 (12): 1390-1396
99. Cadigan KM, Liu YI,. Wnt signaling: Complexity at the surface. J Cell Sci 2006; 119 (3): 395-402
100. Tomilin NV, Solovjeva LV, Svetlova MP, Pleskach NM, Zalenskaya IA, Yau PM, Bradbury EM. Visualization of focal nuclear sites of DNA repair synthesis induced by bleomycin in human cells. Radiat Res 2001; 156 (4): 347-354
101. Giorgetti L, Zanardi A, Venturini S, Carbone R,. Immunocell-array: A novel technology for pathways discovery and cell profiling 2007; 4 (5): 609-616
102. Ronzoni S, Faretta M, Ballarini M, Pelicci P, Minucci S,. New method to detect histone acetylation levels by flow cytometry. Cytometry Part A 2005; 66A (1): 52-61.
103. Perlman ZE, Slack MD, Feng Y, Mitchison TJ, Wu LF, Altschuler SJ,. Multidimensional drug profiling by automated microscopy. Science 2004; 306 (5699): 1194-1198
104. Francavilla C, Cattaneo P, Berezin V, Bock E, Ami D, de, Marco A, Christofori G, Cavallaro U. The binding of NCAM to FGFR1 induces a specific cellular response mediated by receptor trafficking. J Cell Biol 2009; 187 (7): 1101-1116
105. Guilleaume B, Buneß A, Schmidt C, Klimek F, Moldenhauer G, Huber W, Arlt D, Korf U, Wiemann S, Poustka A. Systematic comparison of surface coatings for protein microarrays. Proteomics 2005; 5 (18): 4705-4712
106. 2-treated slides. Methods 2012; 56: 317-325.
107. Bengt K,. Biological surface science. Curr Opin Solid State Mater Sci 1998; 3 (5): 451-459
108. Lipski AM, Jaquiery C, Choi H, Eberli D, Stevens M, Martin I, Chen IW, Sastry PV,. Nanoscale engineering of biomaterial surfaces. Adv Mater 2007; 19 (4): 553-557
109. Rocker C, Potzl M, Zhang F, Parak WJ, Nienhaus GU,. A quantitative fluorescence study of protein monolayer formation on colloidal nanoparticles. Nat Nano 2009; 4 (9): 577-580
110. Nel AE, Mädler L, Velegol D, Xia T, Hoek EMV, Somasundaran P, Klaessig F, Castranova V, Thompson M. Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater 2009; 8 (7): 543-557
111. Roach P, Farrar D, Perry CC,. Interpretation of protein adsorption: Surface-induced conformational changes. J Am Chem Soc 2005; 127 (22): 8168-8173
112. Roach P, Farrar D, Perry CC,. Surface tailoring for controlled protein adsorption: Effect of topography at the nanometer scale and chemistry. J Am Chem Soc 2006; 128 (12): 3939-3945
113. Chayen NE, Saridakis E, Sear RP,. Experiment and theory for heterogeneous nucleation of protein crystals in a porous medium. Proc Natl Acad Sci USA 2006; 103 (3): 597-601
114. Zanardi A, Bandiera B, Bertolini F, Corsini CA, Gregato G, Milani P, Barborini E, Carbone R,. Miniaturized FISH for screening of onco-hematological malignancies. Biotechniques 2010; 49: 497-504.
115. Lenardi C, Perego C, Cassina V, Podestà A, D', Amico A, Gualandris D, Vinati S, Fiorentini F, Bongiorno G, Piserj P, Vellea Sacchi F, Milani P. Adhesion and proliferation of fibroblasts on cluster-assembled nanostructured carbon films: The role of surface morphology. J Nanosci Nanotechnol 2006; 6 (12): 3718-3730
116. Singh AV, Galuzzi M, Borghi F, Vyas V, Podesta A, Indrieri M, Gade WN,. Interaction of bacterial cells with cluster-assembled nanostructured titania surfaces: An atomic force microscopy study. J Nanosci Nanotechnol 2013; 13: 77-85.