Tetramer staining for the detection of HLA-specific B cells

Methods in Molecular Biology

Volumn 1034, Issue , 2013, Pages 313-318

  Lucas, Donna P ^a   Leffell, Mary S ^a   Zachary, Andrea A ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

B lymphocytes; HLA antibodies; HLA sensitization; HLA specific B Lymphocytes; HLA tetramers

Indexed keywords

HLA ANTIGEN; TETRAMER;

ANTIGEN DETECTION; ARTICLE; B LYMPHOCYTE; CELL ISOLATION; HLA SYSTEM; HUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIZATION; STAINING;

EID: 84883216497     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-493-7_17     Document Type: Article

References (6)

1. Leffell M, Zachary A (2012) Antiallograft antibodies: Relevance, detection, and monitoring. Curr Opin Organ Transplant 15:2-7
2. Zachary A, Vega R, Lucas D et al (2010) HLA antibody detection and characterization by solid phase immunoassays-methods and pitfalls. In: Christiansen F, Tait DB (eds) Methods in molecular biology™. Springer, New York, pp 289-308
3. Zachary A, Kopchaliiska D, Montgomery R et al (2007) HLA-specific B cells: Method for their detection, quantification, and isolation using HLA tetramers. Transplantation 83: 982-988
4. Bjorkman P, Saper M, Samraoui B et al (1987) The foreign antigen binding site and T cell recognition regions of class I histocompatibility antigens. Nature 329:512-518
5. Altman J, Moss P, Goulder P et al (1996) Phenotypic analysis of antigen-specific T lymphocytes. Science 274:94-96
6. Zachary A, Kopchaliiska D, Montgomery R et al (2007) HLA-specific B cells: Application to transplantation. Transplantation 83:988-994