Mineral oil-A biofriendly substitute for xylene in deparaffinization: A novel method

Journal of Contemporary Dental Practice

Volumn 14, Issue 2, 2013, Pages 281-286

  Premalatha, B R ^a   Patil, Shankargouda ^a   Rao, Roopa S ^a   Indu, M ^a  

^a M S RAMAIAH UNIVERSITY OF APPLIED SCIENCES   (India)

Author keywords

Deparaffinization; Hematoxylin and eosin; Refined mineral oil; Xylene free deparaffinization; Xylene substitutes

Indexed keywords

EID: 84883177106     PISSN: None     EISSN: 15263711     Source Type: Journal    
DOI: 10.5005/jp-journals-10024-1314     Document Type: Article

References (12)

1. Ankle MR, Joshi PS. A study to evaluate the efficacy of xylene free hematoxylin and eosin staining procedure as compared to conventional hematoxylin and eosin staining: An experimental study. JOMFP 2011;15(2):161-67.
2. Occupational Safety and Health Guideline for Xylene. US Department of Labor. Available from: http://www.osha.gov/ SLTC/healthguidelines/xylene/recognition.html.
3. Kandyala R, Raghavendra SP, Rajasekharan ST. Xylene: An overview of its health hazards and preventive measures. JOMFP 2010;14(1):1-5.
4. Xylene. Available from: http://www.dhs.ca.gov/ohb/HESIS/ xylene.html.
5. Kum S, Sandikci M, Eren U, Metin N. Effects of formaldehyde and xylene inhalations on fatty liver and kidney in adult and developing rats. J Anim Vet Adv 2010;9(2):396-401.
6. Buesa RJ. Mineral oil: The best xylene substitute for tissue processing yet?. J Histotechnol 2000;23(2):143-48.
7. Lin J, Kennedy SH, Svarovsky T, Rogers J, Kemnitz JW, Xu A, et al. High-quality genomic DNA extraction from formalinfixed and paraffin-embedded samples deparaffinized using mineral oil. Anal Biochem 2009;395():265-67.
8. Weber P, Dunlap HL. Solubility of paraffin wax in pure hydrocarbons. Ind Eng Chem 1928;20(4):383-84.
9. Faoláin Eö, Hunter MB, Byrne JM, et al. Raman spectroscopic evaluation of efficiency of current paraffin wax section dewaxing agents. J Histochem Cytochem 2005;53(1):121-29.
10. Falkeholm L, Grant CA, Magnusson A, Möller E, et al. Xylene free method for histological preparation: A multicentre evaluation. Lab Invest 2001;81():1213-21.
11. Chen CY, He T, Mao XL, Friis TE, Qin RH, Jian YT. A novel xylene substitute for histotechnology and histochemistry. Biotech Histochem 2010;85(4):231-40.
12. Buesa RJ, Peshkov MV. Histology without xylene. Ann Diagn Pathol 2009;13():246-56.