Are mouse lens epithelial cells more sensitive to γ-irradiation than lymphocytes?

Radiation and Environmental Biophysics

Volumn 52, Issue 2, 2013, Pages 279-286

  Bannik, Kristina ^a,d   Rössler, Ute ^b   Faus Kessler, Theresa ^a   Gomolka, Maria ^b   Hornhardt, Sabine ^b   Dalke, Claudia ^a   Klymenko, Olena ^a   Rosemann, Michael ^a   Trott, Klaus Rüdiger ^c   Atkinson, Michael ^a   Kulka, Ulrike ^b   Graw, Jochen ^a  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b BUNDESAMT FÜR STRAHLENSCHUTZ   (Germany)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

^d UNIVERSITY HOSPITAL ESSEN   (Germany)

Author keywords

Comet assay; Gamma H2AX assay; Gamma irradiation; Lens epithelial cells; Lymphocytes; Radiation sensitivity

Indexed keywords

ALKALINE COMET ASSAY; COMET ASSAYS; DNA DAMAGE AND REPAIR; DOUBLE-STRAND BREAKS; GAMMA IRRADIATION; LENS EPITHELIAL CELLS; MOLECULAR MECHANISM; RADIATION SENSITIVITY;

CONTACT LENSES; DNA; EXPERIMENTS; LYMPHOCYTES; MAMMALS;

ELECTROCHEMICAL CELLS;

MUS;

GAMMA H2AX PROTEIN, MOUSE; GAMMA-H2AX PROTEIN, MOUSE; HISTONE;

ANIMAL; ARTICLE; C57BL MOUSE; COMET ASSAY; COMPARATIVE STUDY; CYTOLOGY; DNA DAMAGE; EPITHELIUM CELL; GAMMA RADIATION; LENS; LYMPHOCYTE; MALE; METABOLISM; MOUSE; RADIATION EXPOSURE;

ANIMALS; COMET ASSAY; DNA DAMAGE; EPITHELIAL CELLS; GAMMA RAYS; HISTONES; LENS, CRYSTALLINE; LYMPHOCYTES; MALE; MICE; MICE, INBRED C57BL;

EID: 84883055415     PISSN: 0301634X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00411-012-0451-8     Document Type: Article

References (20)

1. Ainsbury EA, Bouffler SD, Dörr W, Graw J, Muirhead C, Edwards AA, Cooper J (2009) Radiation cataractogenesis - a review of recent studies. Radiat Res 172:1-9
2. Bassnett S (2009) On the mechanism of organelle degradation in the vertebrate lens. Exp Eye Res 88:133-139
3. Dalke C, Hölzlwimmer G, Calzada-Wack J, Quintanilla-Martinez L, Atkinson MJ, Rosemann M (2012) Differences in the susceptibility to Iodine 131-induced thyroid tumours amongst inbred mouse strains. J Radiat Res 53:343-353
4. Gajewski AK, Majewska K, Słowikowska MG, Chomiczewski K, Kulig A (1977) Types and rate of cataract development in mice irradiated at different ages. Radiat Res 71:471-480
5. Gomolka M, Rössler U, Hornhardt S, Walsh L, Panzer W, Schmid E (2005) Measurement of the initial levels of DNA damage in human lymphocytes induced by 29 kV X-rays (mammography X-rays) relative to 220 kV X-rays and gamma rays. Radiat Res 163:510-519
6. Graw J, Welzl G, Ahmad N, Klopp N, Heier M, Wulff A, Heinrich J, Döring A, Karrasch S, Nowak D, Schulz H, Rathmann W, Illig T, Peters A, Holle R, Meisinger C, Wichmann HE (2011) The KORA Eye Study: a population-based study on eye diseases in Southern Germany (KORA F4). Invest Ophthalmol Vis Sci 52:7778-7786
7. Jacob S, Boveda S, Bar O, Brézin A, Maccia C, Laurier D, Bernier MO (2012) Interventional cardiologists and risk of radiation-induced cataract: results of a French multicenter observational study. Int J Cardiol (in press)
8. Löbrich M, Shibata A, Beucher A, Fisher A, Ensminger M, Goodarzi AA, Barton O, Jeggo PA (2010) γH2AX foci analysis for monitoring DNA double-strand break repair. Cell Cycle 9:662-669
9. Lukas J, Lukas C, Bartek J (2011) More than just a focus: the chromatin response to DNA damage and its role in genome integrity maintenance. Nat Cell Biol 13:1161-1169
10. Muggleton-Harris AL, Higbee N (1987) Factors modulating mouse lens epithelial cell morphology with differentiation and development of a lentoid structure in vitro. Development 99:25-32
11. Pendergrass W, Zitnik G, Tsai R, Wolf N (2010) X-ray induced cataract is preceded by LEC loss, and coincident with accumulation of cortical DNA, and ROS; similarities with age-related cataracts. Mol Vis 16:1496-1513
12. Pinheiro JC, Bates DM (2000) Mixed-effects models in S and S-plus. Springer, New York
13. Pinheiro J, Bates D, DebRoy S, Sarkar D, The R Core Team (2012) nlme: linear and nonlinear mixed effects models. R Packag Version 3:1-103
14. Rössler U, Hornhardt S, Seidl C, Müller-Laue E, Walsh L, Panzer W, Schmid E, Senekowitsch-Schmidtke R, Gomolka M (2006) The sensitivity of the alkaline comet assay in detecting DNA lesions induced by X rays, gamma rays and alpha particles. Radiat Prot Dosim 122:154-159
15. Shiels A, Bennett TM, Knopf HL, Maraini G, Li A, Jiao X, Hejtmancik JF (2008) The EPHA2 gene is associated with cataracts linked to chromosome 1p. Mol Vis 14:2042-2055
16. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191
17. R Development Core Team (2011) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria; ISBN: 3-900051-07-0. http://www.R-project.org
18. UNSCEAR Report (2006) Effects of ionizing radiation, vol II; annex D - effects of ionizing radiation on the immune system. United Nations, New York
19. Wolf N, Pendergrass W, Singh N, Swisshelm K, Schwartz J (2008) Radiation cataracts: mechanisms involved in their long delayed occurrence but then rapid progression. Mol Vis 14:274-285
20. Worgul BV, Smilenov L, Brenner DJ, Vazquez M, Hall EJ (2005) Mice heterozygous for the ATM gene are more sensitive to both X-ray and heavy ion exposure than are wildtypes. Adv Space Res 35:254-259