Comparison of Pathogen DNA Isolation Methods from Large Volumes of Whole Blood to Improve Molecular Diagnosis of Bloodstream Infections

PLoS ONE

Volumn 8, Issue 8, 2013, Pages

  Loonen, Anne J M ^a,b   Bos, Martine P ^c   van Meerbergen, Bart ^d   Neerken, Sigi ^e   Catsburg, Arnold ^c   Dobbelaer, Irene ^e   Penterman, Roel ^d   Maertens, Geert ^d   van de Wiel, Paul ^e   Savelkoul, Paul ^c   van den Brule, Adriaan J C ^a,b  

^a JEROEN BOSCH HOSPITAL   (Netherlands)

^b FONTYS UNIVERSITY OF APPLIED SCIENCES   (Netherlands)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^d Biocartis SA   (Belgium)

^e PHILIPS RESEARCH LABORATORIES   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM DETECTION; BLOOD VOLUME; BLOODSTREAM INFECTION; CANDIDA ALBICANS; CONTROLLED STUDY; DNA ISOLATION; HUMAN; INTERMETHOD COMPARISON; LYSIS; MOLECULAR DIAGNOSIS; MOLYSIS METHOD; PATHOGENICITY; POLARIS METHOD; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; TRITON TRIS EDTA EASYMAG METHOD; BACTEREMIA; BLOOD; CANDIDIASIS; CHEMISTRY; COMPARATIVE STUDY; DIAGNOSTIC KIT; FUNGEMIA; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR TYPING; PROCEDURES; PSEUDOMONAS INFECTION; STAPHYLOCOCCUS INFECTION;

BACTERIAL DNA; DIAGNOSTIC KIT; FUNGAL DNA;

BACTEREMIA; CANDIDA ALBICANS; CANDIDIASIS; DNA, BACTERIAL; DNA, FUNGAL; FUNGEMIA; HUMANS; MOLECULAR TYPING; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 84881584996     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0072349     Document Type: Article

References (21)

1. van Gestel A, Bakker J, Veraart CP, van Hout BA, (2004) Prevalence and incidence of severe sepsis in Dutch intensive care units. Crit Care 8: R153-162.
2. Vincent JL, Abraham E, (2006) The last 100 years of sepsis. Am J Respir Crit Care Med 173: 256-263.
3. Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, et al. (2004) Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis 39: 309-317.
4. Kumar A, Ellis P, Arabi Y, Roberts D, Light B, et al. (2009) Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock. Chest 136: 1237-1248.
5. Valles J, Rello J, Ochagavia A, Garnacho J, Alcala MA, (2003) Community-acquired bloodstream infection in critically ill adult patients: impact of shock and inappropriate antibiotic therapy on survival. Chest 123: 1615-1624.
6. Sachse S, Straube E, Lehmann M, Bauer M, Russwurm S, et al. (2009) Truncated human cytidylate-phosphate-deoxyguanylate-binding protein for improved nucleic acid amplification technique-based detection of bacterial species in human samples. J Clin Microbiol 47: 1050-1057.
7. Dierkes C, Ehrenstein B, Siebig S, Linde HJ, Reischl U, et al. (2009) Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis 9: 126.
8. Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, et al. (2008) A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 197: 313-324.
9. Tsalik EL, Jones D, Nicholson B, Waring L, Liesenfeld O, et al. (2010) Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. J Clin Microbiol 48: 26-33.
10. Wallet F, Nseir S, Baumann L, Herwegh S, Sendid B, et al. (2010) Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect 16: 774-779.
11. Wellinghausen N, Kochem AJ, Disque C, Muhl H, Gebert S, et al. (2009) Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis. J Clin Microbiol 47: 2759-2765.
12. Kuhn C, Disque C, Muhl H, Orszag P, Stiesch M, et al. (2011) Evaluation of commercial universal rRNA gene PCR plus sequencing tests for identification of bacteria and fungi associated with infectious endocarditis. J Clin Microbiol 49: 2919-2923.
13. Fitting C, Parlato M, Adib-Conquy M, Memain N, Philippart F, et al. (2012) DNAemia Detection by Multiplex PCR and Biomarkers for Infection in Systemic Inflammatory Response Syndrome Patients. PLoS One 7: e38916.
14. Hansen WL, Bruggeman CA, Wolffs PF, (2009) Evaluation of new preanalysis sample treatment tools and DNA isolation protocols to improve bacterial pathogen detection in whole blood. J Clin Microbiol 47: 2629-2631.
15. Horz HP, Scheer S, Huenger F, Vianna ME, Conrads G, (2008) Selective isolation of bacterial DNA from human clinical specimens. J Microbiol Methods 72: 98-102.
16. Peters RP, van Agtmael MA, Gierveld S, Danner SA, Groeneveld AB, et al. (2007) Quantitative detection of Staphylococcus aureus and Enterococcus faecalis DNA in blood to diagnose bacteremia in patients in the intensive care unit. J Clin Microbiol 45: 3641-3646.
17. Loonen AJ, Jansz AR, Kreeftenberg H, Bruggeman CA, Wolffs PF, et al. (2011) Acceleration of the direct identification of Staphylococcus aureus versus coagulase-negative staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods. Eur J Clin Microbiol Infect Dis 30: 337-342.
18. Schneegurt MA, Dore SY, Kulpa CF Jr, (2003) Direct extraction of DNA from soils for studies in microbial ecology. Curr Issues Mol Biol 5: 1-8.
19. Lan J, Walboomers JM, Roosendaal R, van Doornum GJ, MacLaren DM, et al. (1993) Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol 31: 1060-1065.
20. Yanagihara K, Kitagawa Y, Tomonaga M, Tsukasaki K, Kohno S, et al. (2010) Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit. Crit Care 14: R159.
21. Bloos F, Hinder F, Becker K, Sachse S, Mekontso Dessap A, et al. (2010) A multicenter trial to compare blood culture with polymerase chain reaction in severe human sepsis. Intensive Care Med 36: 241-247.