Structure-function relationship in the CYP74 family: Conversion of divinyl ether synthases into allene oxide synthases by site-directed mutagenesis

FEBS Letters

Volumn 587, Issue 16, 2013, Pages 2552-2558

  Toporkova, Yana Y ^a   Ermilova, Valeria S ^a   Gorina, Svetlana S ^a   Mukhtarova, Lucia S ^a   Osipova, Elena V ^a   Gogolev, Yuri V ^a   Grechkin, Alexander N ^a  

^a KAZAN INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Russian Federation)

Author keywords

Allene oxide synthase; CYP74 family; Cytochrome P450; Divinyl ether synthase; Site directed mutagenesis

Indexed keywords

12 OXO 10,15 PHYTODIENOIC ACID; ALLENE OXIDE SYNTHASE; ALPHA KETOL DERIVATIVE; CHEMICAL COMPOUND; CYTOCHROME P450; DIVINYL ETHER SYNTHASE; GLUTAMIC ACID; GLYCINE; MUTANT PROTEIN; PHENYLALANINE; PROTEIN CYP74; PROTEIN CYP74D3; SYNTHETASE; UNCLASSIFIED DRUG; VALINE; VEGETABLE PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MODIFICATION; ENZYME STRUCTURE; FLAX; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN FAMILY; SITE DIRECTED MUTAGENESIS; TOBACCO; WILD TYPE;

(9S,10E,12Z)-9-HYDROPEROXY-10,12-OCTADECADIENOIC ACID; (9Z,11E,13S,15Z)-13-HYDROPEROXY-9,11,15-OCTADECATRIENOIC ACID; 13-HPOT; 9-HPOD; ALLENE OXIDE SYNTHASE; AOS; CYP74 FAMILY; CYTOCHROME P450; DES; DIVINYL ETHER SYNTHASE; HPL; HYDROPEROXIDE LYASE; I-HELIX CENTRAL DOMAIN; IHCD; IMAC; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LINUM USITATISSIMUM DES (CYP74B16); LUDES; ME; METHYL; NICOTIANA TABACUM DES (CYP74D3); NTDES; SITE-DIRECTED MUTAGENESIS; SRS; SUBSTRATE RECOGNITION SITE; TMS; TRIMETHYLSILYL; WILD TYPE; WT;

AMINO ACID SEQUENCE; COMPUTATIONAL BIOLOGY; CYTOCHROME P-450 ENZYME SYSTEM; FLAX; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; INTRAMOLECULAR OXIDOREDUCTASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLANT PROTEINS; POINT MUTATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP; TOBACCO;

INVERTEBRATA; LINUM USITATISSIMUM; NICOTIANA TABACUM;

EID: 84881481705     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2013.06.030     Document Type: Article

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