Use of bronchoalveolar lavage to assess the respiratory microbiome: Signal in the noise

The Lancet Respiratory Medicine

Volumn 1, Issue 5, 2013, Pages 354-356

  Twigg III, Homer L ^a   Morris, Alison ^b   Ghedin, Elodie ^b   Curtis, Jeffrey L ^c,d   Huffnagle, Gary B ^c   Crothers, Kristina ^e   Campbell, Thomas B ^f   Flores, Sonia C ^f   Fontenot, Andrew P ^f   Beck, James M ^d   Huang, Laurence ^g   Lynch, Susan ^g   Knox, Kenneth S ^h   Weinstock, George ^e  

^a INDIANA UNIVERSITY MEDICAL CENTER   (United States)

^b UNIVERSITY OF PITTSBURGH   (United States)

^c UNIVERSITY OF MICHIGAN   (United States)

^d VETERANS AFFAIRS MEDICAL CENTER   (United States)

^e UNIVERSITY OF WASHINGTON   (United States)

^f UNIVERSITY OF COLORADO   (United States)

^g UNIVERSITY OF CALIFORNIA   (United States)

^h UNIVERSITY OF ARIZONA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ASTHMA; BACTERIAL TRANSLOCATION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; LOWER RESPIRATORY TRACT; LUNG FLUID; MICROBIAL COLONIZATION; MICROBIAL COMMUNITY; MICROBIAL CONTAMINATION; MICROBIOME; NONHUMAN; NOTE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEOBACTERIA; RNA SEQUENCE; TROPHERYMA WHIPPELII; UPPER RESPIRATORY TRACT; LUNG; LUNG LAVAGE; MICROBIOLOGY; MICROFLORA; PROCEDURES;

BRONCHOALVEOLAR LAVAGE; BRONCHOALVEOLAR LAVAGE FLUID; HUMANS; LUNG; MICROBIOTA;

EID: 84881333761     PISSN: 22132600     EISSN: None     Source Type: Journal    
DOI: 10.1016/S2213-2600(13)70117-6     Document Type: Letter

References (15)

1. Hamady M, Knight R. Microbial community profiling for human microbiome projects: tools, techniques, and challenges. Genome Res 2009; 19: 1141-52.
2. Dewhirst FE, Chen T, Izard J, et al. The human oral microbiome. J Bacteriol 2010; 192: 5002-17.
3. Charlson ES, Bittinger K, Haas AR, et al. Topographical continuity of bacterial populations in the healthy human respiratory tract. Am J Respir Crit Care Med 2011; 184: 957-63.
4. Gleeson K, Eggli DF, Maxwell SL. Quantitative aspiration during sleep in normal subjects. Chest 1997; 111: 1266-72.
5. Lozupone C, Cota-Gomez A, Palmer BE, et al. Widespread colonization of the lung by Tropheryma whipplei in HIV infection. Am J Respir Crit Care Med 2013; 187: 1110-17.
6. Morris A, Beck JM, Schloss PD, et al. Comparison of the respiratory microbiome in healthy nonsmokers and smokers. Am J Respir Crit Care Med 2013; 187: 1067-75.
7. Sloan WT, Lunn M, Woodcock S, Head IM, Nee S, Curtis TP. Quantifying the roles of immigration and chance in shaping prokaryote community structure. Environ Microbiol 2006; 8: 732-40.
8. Charlson ES, Bittinger K, Chen J, et al. Assessing bacterial populations in the lung by replicate analysis of samples from the upper and lower respiratory tracts. PLoS One 2012; 7: e42786.
9. Sze MA, Dimitriu PA, Hayashi S, et al. The lung tissue microbiome in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 2012; 185: 1073-80.
10. Erb-Downward JR, Thompson DL, Han MK, et al. Analysis of the lung microbiome in the "healthy" smoker and in COPD. PLoS One 2011; 6: e16384.
11. Huang YJ, Nelson CE, Brodie EL, et al. Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma. J Allergy Clin Immunol 2011; 127: 372-81.
12. Miller M Twigg HL III, Petrache I. The role of Gram negative bacteria in the susceptibility of mice to an asthmatic phenotype. Am J Respir Crit Care Med 2011; 183: A6207.
13. Shen M, Preston AM, Gillilland MG, et al. Characterization of the lung microbiome of specifi c pathogen-free mice with and without exposure to cigarette smoke in vivo. Am J Respir Crit Care Med 2011; 183: A6210.
14. Fujimura KE, Slusher NA, Cabana MD, Lynch SV. Role of the gut microbiota in defi ning human health. Expert Rev Anti Infect Ther 2010; 8: 435-54.
15. Hilty M, Burke C, Pedro H, et al. Disordered microbial communities in asthmatic airways. PLoS One 2010; 5: e8578.